Method for culturing new variety of smilax riparia with tissue mutation of flower stalks

A cultivation method and technology for new varieties are applied in the field of cultivation of new varieties for inducing mutation of flower stalk tissue of oxtail, and can solve the problems of endangered wild resources of oxtail, low yield of artificial cultivation, and plant degradation, and achieve clear sources of single plant and single plant. The effect of high purity of genetic information and high mutation rate of strains

Active Publication Date: 2020-06-19
通化天妍生物技术有限公司 +1
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Problems solved by technology

[0005] In order to solve the problems that the above-mentioned wild resources of oxtail are on the verge of extinction, the yield of artificial cultivation is low, and it is prone to poor germination,...

Method used

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  • Method for culturing new variety of smilax riparia with tissue mutation of flower stalks
  • Method for culturing new variety of smilax riparia with tissue mutation of flower stalks
  • Method for culturing new variety of smilax riparia with tissue mutation of flower stalks

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Embodiment Construction

[0027] The technical solution in the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

[0028] Such as figure 1 and 2 Shown, a kind of new variety cultivation method of inducing oxtail cauliflower flower stalk tissue mutation, comprises the following steps:

[0029] 1 Materials and methods

[0030] 1.1 Material selection and pretreatment

[0031] In mid-to-late June, after the wild oxtail umbels are released, select the total flower stalk, cut and leave a length of 3.0 cm, rinse with 75% ethanol (volume percentage concentration) for 10 seconds, move to saturated sodium hypochlorite solution for 3 minutes, and then soak in sterile water. Rinse 8 times, blot the surface moisture with sterile filter paper for later use.

[0032] 1.2 Method

[0033] 1.2.1 Preparation of et...

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Abstract

The invention belongs to the technical field of planting reproduction and in particular relates to a method for culturing a new variety of smilax riparia with tissue mutation of flower stalks. According to the method for culturing the new variety of smilax riparia with tissue mutation of the flower stalks, smilax riparia flower stalks are adopted as a material which is soaked into ethylmethane sulfonate of an appropriate concentration for a certain time, and furthermore, by using a plant tissue culture technology, and methods of flower stalk tissue adventitious bud induction, adventitious budrooting and rejuvenation, and the like, a novel artificial cultivated variety of smilax riparia which has the characteristics of being good in single plant independence, high in single plant genetic information purity, clear in single plant source, and the like, can be obtained. The variety is stable in genetic property and can be cultivated as a strain to construct a smilax riparia mutant library.

Description

technical field [0001] The invention belongs to the technical field of new varieties of plants, and in particular relates to a method for cultivating new varieties by inducing mutations in the pedicel tissue of oxtail. Background technique [0002] Oxtail vegetable (Smilax riparia A.DC.) is a perennial herbaceous vine of the genus Smilax in the family Liliaceae. Alternative names: sarsaparilla, sarsaparilla, whipped vegetable, melaleuca pagoda, asparagus, stem length 1-2 meters, hollow, with a small amount of pith, sunken and grooved after drying. The leaves are thicker than the previous species, and the shape varies greatly; the petiole is 7-20 mm long, and usually has tendrils below the middle. Umbel peduncle slender, 3-5(-10) cm long. It is mainly distributed in Northeast China, North Korea and Japan, and grows in forests, shrubs, ravines or hillside grasses below 1600 meters above sea level. Oxtail is a wild resource plant that integrates edible, medicinal and industr...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/06
CPCA01H4/008A01H1/06
Inventor 顾地周李莹付智缘
Owner 通化天妍生物技术有限公司
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