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A method for raising seedlings of subgenus Azalea by using immature embryos

A technology of azalea and immature embryos, which is applied in botany equipment and methods, planting substrates, applications, etc., can solve the problems of long seedling raising cycle, achieve the effects of low cost, simple operation, and shortened seedling time

Active Publication Date: 2022-06-21
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the sowing and seedling raising of plants of the subgenus Azalea is to collect mature capsules that have cracked, and take mature seeds for sowing and raising seedlings. The plants bloom from March to May, and the fruits are not mature until November. The fruit maturity period is too long, and due to factors such as seed dormancy, the Seeds can only germinate in the second spring (April-May). It takes about 5 months for the seeds to mature and sow. The breeding and seedling cycle is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 The method of azalea's natural pollination of 5-month-old young embryos for breeding and raising seedlings

[0032] 1. Peeling of young embryos: Take the 5-month-old green capsules of Yingshanhong after 5 months of natural pollination, rinse them under running water for 5 minutes, and dry them with scalpels and tweezers. removal of immature embryos;

[0033] 2. Pretreatment of immature embryos: Immerse and disinfect immature embryos in 75% ethanol for 1 min, blot dry with absorbent paper, and place them in a centrifuge tube. Add 800 mg / l GA that is currently prepared and stored in the dark. 3 solution, centrifuged and stirred evenly, and then soaked in the dark for 24 hr.

[0034] 3. Preparation and sowing of seedling trays: Under greenhouse conditions, prepare a seedling tray with a depth of 75 mm and a bottom covered with drainage holes, containing 40 - 50 mm thick fiber length 0 - 10 mm, pH value 5.5, EC value 20 mS / m of peat specially used for seedli...

Embodiment 2

[0037] Embodiment 2 The method of azalea naturally pollinating 6-month-old young embryos for breeding and raising seedlings

[0038] 1. Peeling of young embryos: Take the 6-month-old green capsules of Yingshanhong after 6 months of natural pollination, rinse them under running water for 5 minutes, and dry them with a scalpel and tweezers. removal of immature embryos;

[0039] 2. Pretreatment of immature embryos: Immerse and disinfect immature embryos in 75% ethanol for 1 min, dry them with absorbent paper and place them in a centrifuge tube, add 600 mg / l GA that is currently prepared and stored in the dark 3 solution, centrifuged and stirred evenly, and then soaked in the dark for 24 hr.

[0040] Items 3 and 4 are the same as in Example 1.

[0041] 5. Management and transplanting at the seedling stage: after 25 days, the film is removed, and the ratio of nitrogen, phosphorus and potassium is 30: 10: 20 for 60 days, and the weight ratio is water: compound fertilizer = 1000: 1...

Embodiment 3

[0042] Example 3 Method for raising seedlings of young ovules after 5 months of artificial hybridization and pollination of azalea subgenus

[0043] 1. Peeling of young embryos: Take the full-grown green capsules of the subgenus Yingshanhong after 5 months of artificial cross-pollination, rinse them under running water for 5 minutes, and dry them with a scalpel and tweezers. immature embryo;

[0044] Items 2 and 3 are the same as in Embodiment 1;

[0045] 4. Management at the emergence stage: The seedling tray is covered with a transparent plastic film, and cultivated in a greenhouse under normal light and a temperature of 20 - 25 °C;

[0046] 5. Management and transplanting at the seedling stage: after 35 days, the film is removed, and the ratio of nitrogen, phosphorus and potassium is 30: 10: 20 for 60 days, and the weight ratio is water: compound fertilizer = 1000: 1-2 compound fertilizer once, greenhouse Under normal temperature maintenance and management; seedlings with...

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Abstract

The invention discloses a method for raising seedlings of plants of the subgenus Azalea by using immature embryos. In the method, immature embryos aged 5 months (and above) after 5 months of pollination are taken, and subjected to GA 3 After pretreatment, the seedlings are sown in the greenhouse, and the specific steps include: (1) Stripping of young embryos, (2) Pretreatment of young embryos, (3) Sowing, (4) Emergence management, (5) Seedling management and transplanting . The invention shortens the time from pollination to seedling formation of subgenus azalea, thereby effectively shortening the breeding and growth cycle of subgenus azalea, and provides a simple and easy new way for breeding and seedlings of subgenus azalea, which is simple to operate, low in cost, and can be It is popularized and applied in the introduction and domestication of plants of the subgenus Azalea, hybrid breeding, and seedling production.

Description

technical field [0001] The invention relates to a method for raising seedlings of azalea subgenus by utilizing immature embryos, and belongs to the technical field of plant propagation and seedling raising. Background technique [0002] Azalea (Subgen. Tsutsusi ) belongs to the genus Rhododendron (Ericaceae) Rhododendron ), erect shrub, this subgenus has about 90 wild species and more than 20,000 horticultural species. It is rich in species, various colors, beautiful in appearance, and has high ornamental value. [0003] The wild resources of the subgenus Azalea are mainly distributed in the middle and lower reaches of the Yangtze River and the south of China. It is reported that there are 72 species and 6 varieties in my country, accounting for 80% of the subgenus. The role of population and ecological maintenance cannot be underestimated, and the landscape is magnificent in the flowering season, which has a high landscape value. The subgenus of azalea is rich in resour...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G17/00A01C1/00A01C1/08A01G24/28A01G24/10A01G24/30C05G3/00C05G5/23
CPCA01G31/00A01G17/005A01C1/00A01C1/08A01G24/28A01G24/10A01G24/30C05C5/02C05G3/00
Inventor 李畅苏家乐刘晓青何丽斯肖政周惠民孙晓波
Owner JIANGSU ACAD OF AGRI SCI
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