Method for detecting influence of microbes to plant roots and special device for implementing same

Abstract

The invention discloses a method for detecting the influence of microbes to plant roots and a special device for implementing the same. The method includes the following steps: (I) adding a microbe to be detected and culture solution to a box body (2); (II), putting a germination plate (1) on the top ends of partition plates inside the box body, and putting a piece of wet germination paper on the germination plate; (III), putting plant breeding materials on the germination plate, wherein each plant breeding material corresponds to one through hole of the germination plate; and (IV), covering the plant reproduction materials with a culture matrix, culturing the plant reproduction materials until the plant reproduction materials root, and observing and detecting the growth of plant roots. The method has a very broad application prospect in screening resistance varieties of plants and evaluating the prevention and control effect of bactericides on disease of plant roots.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for detecting the influence of microorganisms on plant root systems and a special device thereof, and the application of the method in screening plant resistant and susceptible varieties and evaluating the control effect of fungicides on plant root system diseases. Background technique [0002] Traditionally, the pot method is used to detect pathogenic microorganisms of plant root diseases and to screen plant resistant and susceptible varieties. The potting method generally uses soil as the medium, and it is difficult to realize the visual observation and evaluation of the growth changes of plant roots and the corresponding stress of plants and microorganisms, and it takes a long time. Generally, it takes about 20 days from inoculation to the symptoms of the aboveground parts; The degree of damage caused by strains of pathogenic bacteria to plant roots is signific...

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Problems solved by technology

The potting method generally uses soil as the medium, and it is difficult to realize the visual observation and evaluation of the growth changes of plant roots and the corresponding stress of plants and microorganisms, and it takes a long time. Generally, it takes about 20 days from inoculation to the symptoms of the aboveground parts; The degree of damage caused by strains of pathogenic bacteria to plant roots is significantly different, while the aboveground parts of plants in the potted method have no obvious difference in a short period of time, which makes it difficult to distinguish the strength of pathogenic microorganisms; at the same time, the potted method generally uses soil as the medium, which cannot Guarantees complete contact between plant roots and pathogenic microorganisms, even under the same inoculation conditions, the reproducibility and stability of test results are low

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