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Method for overcoming vitrification in shoot tip culture of carnations by using high-molecular water-absorbent resin

A polymer water-absorbing, carnation technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of insignificant effect and ineffective effect of carnation red varieties, and achieve the effect of reducing humidity in the bottle

Active Publication Date: 2016-02-17
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it has little effect on carnation red varieties, and it has no obvious effect on other color varieties, and has no effect on the vitrification phenomenon of carnation virus-free seedlings.

Method used

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  • Method for overcoming vitrification in shoot tip culture of carnations by using high-molecular water-absorbent resin
  • Method for overcoming vitrification in shoot tip culture of carnations by using high-molecular water-absorbent resin

Examples

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Effect test

Embodiment 1

[0028] 1) Select "Master" carnation plants with strong growth, good quality and no pests and diseases, select strong lateral buds at the base, peel off the unfolded leaves, leave 2-3 pairs of leaves unexpanded, and cut off the 2cm stem section with the top , placed in a clean beaker for later use;

[0029] 2) Sterilize on an ultra-clean workbench, pour 0.1% mercury liter solution, and shake gently to make the plant material contact with the sterilized liquid evenly. After sterilizing for 8 minutes, rinse it with sterile water for 3-5 times, and set aside ;

[0030] 3) Under the dissecting microscope, 0.5mm of carnation stem tip is stripped and inserted into a 100ml bottle with 40ml / bottle of culture medium. L+NAA1g / L+0-24g / L high molecular weight water-absorbing resin, after receiving 4 plants in each bottle, cultivate them at 26℃±0.5℃, light intensity 1000lx-4000lx, from August 10, 2013 to August 30 day, statistical results;

[0031] Specifically, a total of 4 treatments w...

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Abstract

The invention belongs to the field of plant regeneration, and provides a method for overcoming vitrification in shoot tip culture of carnations by using high-molecular water-absorbent resin. The method comprises the following steps: 1) selecting healthily growing carnation plants, and cutting stem segments of 2cm with tips; 2) washing for 3-5 times with sterile water for standby; and 3) peeling carnation shoot tips of 0.5mm and inoculating the shoot tips on a medium; and the medium comprises the following components: ferric salt double-MS, 30g / L of saccharose, 6g / L of agar, 1g / L of TDZ, 1g / L of NAA and 8g / L of high-molecular water-absorbent resin; the shoot tips are cultivated at 20-30 DEG C. Tests are carried out for determining medium additives, and after test comparison, concentrations of additives are determined. Test results show that high-molecular water-absorbent resin with the appropriate concentration can be used for reducing the vitrification occurrence rate of virus free red carnation shoot tips to about 17%, and subsequent vitrification generating cases of the plants without vitrification are also greatly reduced.

Description

technical field [0001] The invention belongs to the field of plant regeneration, and in particular relates to a tissue culture method for overcoming the vitrification of shoot tip culture. Background technique [0002] Vitrification, another common problem in tissue culture, is a physiological disorder of tissue culture plantlets. When plant materials are continuously propagated in vitro, the tender stems and leaves of some cultures tend to be translucent and water-soaked. This phenomenon is usually called vitrification. It will cause the growth of tissue cultured seedlings to be slow or deformed, the reproduction coefficient will be reduced, and the seedlings cannot be transplanted to survive. It occurs in both herbaceous and woody plants, and it has been reported that more than 70 plants have glass seedlings. The leaves of glass seedlings are crystal transparent or translucent, water-soaked, the plants are short and swollen, the color is green, the leaves are shrunken an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘春彭佳佳杨哲李佩愉明军袁素霞
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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