Preparation method of peony callus extract with high paeoniflorin content
A technology of callus and extract, applied in the field of preparation of peony callus extract, can solve the problems of low content of active substances, easy browning of callus, low induction differentiation rate and yield of peony callus, etc. Achieve the effect of reducing the growth of bacteria and increasing the induction rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0026] A preparation method of a peony callus extract with high paeoniflorin content, comprising the following steps:
[0027] S1, preparation of peony explants:
[0028] a. Select peony seeds, place them at the bottom of a beaker, soak them in pure water at 35-45°C, add pure water to half the height of the seeds, then cover them with gauze, and replace them every 24 hours until cracks appear on the hard shell on the surface of the seeds, forming a germination state The seeds are A product. This step is to promote the seeds to enter the germination state, and the appearance of cracks means that the seeds are ready to germinate.
[0029] b. Take product A with a better germination state, wash it thoroughly, put it in a beaker, cover it with gauze, and rinse it with running water for a long time. After washing, fully soak product A with a concentration of 2-10% NaClO solution, and then put it in Disinfect by rotating and shaking on a multifunctional rotary shaker for 20-40 min...
Embodiment 1
[0037] A preparation method of a peony callus extract with high paeoniflorin content, comprising the following steps:
[0038] S1, preparation of peony explants:
[0039] a. Select peony seeds, place them at the bottom of a beaker, soak them in pure water at 40°C, add pure water to half the height of the seeds, then cover them with gauze, and replace them every 24 hours until cracks appear on the hard shell on the surface of the seeds, forming germinated seeds , for A product. This step is to promote the seeds to enter the germination state, and the appearance of cracks means that the seeds are ready to germinate.
[0040] b. Take product A with a good germination state, wash it thoroughly, put it in a beaker, cover it with gauze, and rinse it with running water for a long time. After washing, fully soak product A in a 5% NaClO solution, and then put it in Rotate and vibrate on a rotary shaker for 30 minutes, wash it with sterile water, and perform the first disinfection and...
Embodiment 2
[0046] A preparation method of a peony callus extract with high paeoniflorin content, comprising the following steps:
[0047] S1, preparation of peony explants:
[0048] a. Select peony seeds, place them at the bottom of a beaker, soak them in pure water at 40°C, add pure water to half the height of the seeds, then cover them with gauze, and replace them every 24 hours until cracks appear on the hard shell on the surface of the seeds, forming germinated seeds , for A product. This step is to promote the seeds to enter the germination state, and the appearance of cracks means that the seeds are ready to germinate.
[0049] b. Take product A with a good germination state, wash it thoroughly, put it in a beaker, cover it with gauze, and rinse it with running water for a long time. After washing, fully soak product A in a 5% NaClO solution, and then put it in Rotate and vibrate on a rotary shaker for 30 minutes, wash it with sterile water, and perform the first disinfection and...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com