Preparation method of peony callus extract with high paeoniflorin content

A technology of callus and extract, applied in the field of preparation of peony callus extract, can solve the problems of low content of active substances, easy browning of callus, low induction differentiation rate and yield of peony callus, etc. Achieve the effect of reducing the growth of bacteria and increasing the induction rate

Active Publication Date: 2022-01-14
ZHEJIANG YIGE ENTERPRISE MANAGEMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the traditional plant callus culture method is used to produce peony callus at present, which is easily disturbed by the external environment and polluted by bacteria, which affects its survival rate,

Method used

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  • Preparation method of peony callus extract with high paeoniflorin content

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preparation example Construction

[0026] A preparation method of a peony callus extract with high paeoniflorin content, comprising the following steps:

[0027] S1, preparation of peony explants:

[0028] a. Select peony seeds, place them at the bottom of a beaker, soak them in pure water at 35-45°C, add pure water to half the height of the seeds, then cover them with gauze, and replace them every 24 hours until cracks appear on the hard shell on the surface of the seeds, forming a germination state The seeds are A product. This step is to promote the seeds to enter the germination state, and the appearance of cracks means that the seeds are ready to germinate.

[0029] b. Take product A with a better germination state, wash it thoroughly, put it in a beaker, cover it with gauze, and rinse it with running water for a long time. After washing, fully soak product A with a concentration of 2-10% NaClO solution, and then put it in Disinfect by rotating and shaking on a multifunctional rotary shaker for 20-40 min...

Embodiment 1

[0037] A preparation method of a peony callus extract with high paeoniflorin content, comprising the following steps:

[0038] S1, preparation of peony explants:

[0039] a. Select peony seeds, place them at the bottom of a beaker, soak them in pure water at 40°C, add pure water to half the height of the seeds, then cover them with gauze, and replace them every 24 hours until cracks appear on the hard shell on the surface of the seeds, forming germinated seeds , for A product. This step is to promote the seeds to enter the germination state, and the appearance of cracks means that the seeds are ready to germinate.

[0040] b. Take product A with a good germination state, wash it thoroughly, put it in a beaker, cover it with gauze, and rinse it with running water for a long time. After washing, fully soak product A in a 5% NaClO solution, and then put it in Rotate and vibrate on a rotary shaker for 30 minutes, wash it with sterile water, and perform the first disinfection and...

Embodiment 2

[0046] A preparation method of a peony callus extract with high paeoniflorin content, comprising the following steps:

[0047] S1, preparation of peony explants:

[0048] a. Select peony seeds, place them at the bottom of a beaker, soak them in pure water at 40°C, add pure water to half the height of the seeds, then cover them with gauze, and replace them every 24 hours until cracks appear on the hard shell on the surface of the seeds, forming germinated seeds , for A product. This step is to promote the seeds to enter the germination state, and the appearance of cracks means that the seeds are ready to germinate.

[0049] b. Take product A with a good germination state, wash it thoroughly, put it in a beaker, cover it with gauze, and rinse it with running water for a long time. After washing, fully soak product A in a 5% NaClO solution, and then put it in Rotate and vibrate on a rotary shaker for 30 minutes, wash it with sterile water, and perform the first disinfection and...

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Abstract

The invention discloses a preparation method of a peony callus extract with high paeoniflorin content. The preparation method comprises the following steps: S1, preparing a peony explant: a, taking peony seeds, soaking the peony seeds with water, and covering the peony seeds with gauze until cracks appear on the surfaces of the seeds to obtain products A; b, washing the products A, then covering the products A with gauze, and disinfecting and degerming the products A twice after washing to obtain products B; c, transferring the products B into a germination culture medium for culturing until the seeds germinate seedlings to obtain products C; S2, induction of peony callus: putting the products C into an induction culture medium for callus induction culture to obtain products D; S3, culture of subculture peony callus: transferring the products D into a subculture medium for amplification culture, and carrying out subculture on schedule to obtain products E; and S4, preparation of the extract: preparing the products E into the peony callus extract to obtain a finished product. The preparation method has the characteristics of high inductivity, low browning and vitrification rate and high active substance content.

Description

technical field [0001] The invention relates to a preparation method of an extract, in particular to a preparation method of a peony callus extract with high paeoniflorin content. Background technique [0002] Peony (Paeonia lactiflora Pall.) is a perennial herbaceous plant belonging to the family Ranunculaceae and the genus Paeoniae. Its extract is rich in paeoniflorin, paeonol, paeoniflorin, oxidized paeoniflorin, benzoylpaeoniflorin and other components, among which paeoniflorin has the highest content. Paeoniflorin has excellent whitening, moisturizing, and antioxidant activities, and also has anti-inflammatory, anti-aging, anti-allergic, and skin-healthy effects, and is very suitable for research as a cosmetic raw material. At present, the main production method of paeoniflorin is to extract paeoniflorin roots that have been dried and processed for 2 to 4 years, and then separated to obtain paeoniflorin. The traditional production method of peony is mostly branch prop...

Claims

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Application Information

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IPC IPC(8): A01H4/00A61K8/9789A61Q19/00A61Q19/02A61Q19/08
CPCA01H4/005A01H4/002A61K8/9789A61Q19/005A61Q19/08A61Q19/02A61K2800/805
Inventor 张大勇毕永贤孔德承周浩淼李昊占小虎朱秀洁
Owner ZHEJIANG YIGE ENTERPRISE MANAGEMENT GRP CO LTD
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