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Preparation technology of muscular amino acids and peptides and nucleoside injection liquid

A technology of carnosine glycoside injection and water for injection, which is applied in the field of biochemical pharmacy for the preparation of cardiovascular and cerebrovascular diseases, can solve the problems that the mold temperature cannot be too high, and the preparation is easy to "grow bacteria", so as to reduce economic losses and improve product quality. Purity, the effect of ensuring drug safety

Inactive Publication Date: 2009-09-23
长春白求恩制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for preparing sarcosaminoglycan glycoside injection, which overcomes the disadvantages that the mold temperature in the existing preparation process cannot be too high and the preparation is easy to "grow bacteria"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] After removing the connective tissue, the heart and muscle of rabbits that have passed the quarantine are mixed with water for injection at a ratio of 0.8:1, homogenized once with a high-speed tissue grinder, placed in a -20°C freezer, and thawed at room temperature after freezing for 30 hours. , repeated 3 times, the last melted tissue fluid was kept in a constant temperature water bath at a constant temperature of 55°C for 4 hours, the pH value was adjusted to 7.0, 3g / L activated carbon was added according to the weight of the tissue fluid, boiled for 20 minutes, and then centrifuged at 3000rpm at low temperature for 30 minutes. The pH value of the supernatant liquid is adjusted to 5.5, and 2g / liter of chitosan oligosaccharides is added at room temperature, then the pH value is adjusted to 7.2, filtered with a 0.22um microporous membrane, 10,000 molecular weight ultrafiltration membrane ultrafiltration to obtain muscle Aminopeptidoside solution. After measuring the co...

Embodiment 2

[0012] After removing the connective tissue from the heart and muscle of rabbits that have passed the quarantine, mix them with water for injection at a ratio of 0.78:1, homogenize once with a high-speed tissue masher, put them in a -20°C freezer, and freeze for 38 hours Melt at room temperature and repeat 3 times. The last thawed tissue fluid was kept in a constant temperature water bath at a constant temperature of 55°C for 3 hours. Adjust the pH value to 7.0. Add 1 g / L of activated carbon according to the weight of the tissue fluid. After boiling for 20 minutes, centrifuge at 3000 rpm for 30 minutes at low temperature. The supernatant liquid adjusts the pH value to 5.5, adds 1.5g / liter chitosan oligosaccharide in room temperature, then adjusts the pH value to 7.2, filters with 0.22um microporous membrane, 10,000 molecular weight ultrafiltration membrane ultrafiltration obtains muscle Aminopeptidoside solution. After measuring the content of polypeptide and hypoxanthine, add...

Embodiment 3

[0014] After removing the connective tissue, the heart and muscle of rabbits qualified for quarantine are mixed with water for injection at a ratio of 0.82:1, homogenized with a high-speed tissue masher, placed in a -20°C freezer, frozen for 50 hours, and then melted at room temperature , repeated 3 times, the last melted tissue fluid was kept in a constant temperature water bath at a constant temperature of 55°C for 4 hours, the pH value was adjusted to 7.0, and activated carbon was added according to the weight of the tissue fluid at 2g / liter, boiled for 20 minutes and then centrifuged at 3000rpm at low temperature for 30 minutes. Adjust the pH value of the supernatant liquid to 6.0, add 2g / liter chitosan oligosaccharide at room temperature, then adjust the pH value to 7.2, filter with a 0.22um microporous membrane, and obtain sarcosine by ultrafiltration with a 10,000 molecular weight ultrafiltration membrane Peptide solution. After measuring the content of polypeptide and ...

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PUM

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Abstract

A process for preparing the injection of carnosine features that the chitosan and oligose are used for adsorbing the modified protein and fat to improve the purity of product, and the 6-aminopurine phosphate is used as the protector for increasing the sterilizing temp and time to 121 deg.C and 30 min, so having no bacterial pollution.

Description

technical field [0001] The invention improves the existing production process of carnosine glycoside injection, provides a preparation method of carnosine glycoside injection, and belongs to the technical field of biochemical pharmacy for preparing and treating cardiovascular and cerebrovascular diseases. Background technique [0002] Carnosine glycoside injection is used for brain dysfunction; cerebral apoplexy, brain hypofunction caused by insufficient blood supply to the brain; peripheral nerve diseases, etc., with remarkable curative effect and minimal side effects. [0003] However, since carnosine injection is a mixture of biochemical substances, polypeptides, nucleosides and nucleotides are unstable at high temperatures and often precipitate, so the sterilization temperature of the preparation in the final sterilization stage generally cannot Above 105°C, the inactivation temperature of microbial spores cannot be reached. The early fluctuations in the production proc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K35/34A61P9/10A61P25/00
Inventor 王巍
Owner 长春白求恩制药有限公司
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