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Preparation technology of liver hydrolytic peptide injection liquid

A liver hydrolyzed peptide and injection technology, which is applied in the field of biochemical pharmacy, can solve the problems of mold temperature not being too high, bacteria growth, etc., to reduce economic losses, improve product purity, and ensure drug safety.

Inactive Publication Date: 2009-09-23
长春白求恩制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention discloses a preparation method of hepatic hydrolyzed peptide injection, which overcomes the disadvantages that the mold temperature in the existing preparation process cannot be too high and the pharmaceutical preparation is easy to "grow bacteria"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] After removing the connective tissue, the bovine liver qualified for quarantine is mixed with water for injection at a weight ratio of 0.80:1, homogenized by a high-speed tissue grinder, heated to 51°C, and washed with Ca(OH) 2 Adjust the pH value to 7.2, add trypsin at 4g / liter, hydrolyze at 50-51°C for 1 hour, add A.T.3942 enzyme 1g / liter, continue hydrolysis for 4 hours, adjust the pH value of the hydrolyzate to 5.8-6.0 with phosphoric acid, add 1g / liter of activated carbon, heat to boil, keep warm for 30 minutes, and centrifuge at 3000rpm at low temperature for 30 minutes. Take the supernatant and add 2g / liter chitosan oligosaccharide at room temperature to form a precipitate, remove the precipitate by filtration, then adjust the pH value to 7.2 with NaOH, filter with a 0.22 μm microporous membrane, and filter with a 10,000 molecular weight ultrafiltration membrane. The hepatic hydrolyzed peptide solution was obtained by filtration, and after measuring the peptide...

Embodiment 2

[0012] After removing the connective tissue, the pig liver qualified for quarantine is mixed with water for injection at a weight ratio of 0.82:1, homogenized by a tissue masher, heated to 51°C, and washed with Ca(OH) 2 Adjust the pH value to 7.0, add trypsin at 4g / liter, hydrolyze at 50-51°C for 1 hour, add A.T.3942 enzyme, continue hydrolysis for 5 hours, adjust the pH value of the hydrolyzate to 5.8-6.0 with phosphoric acid, add 1g / liter activated carbon , heat to boil, keep warm for 30 minutes, and centrifuge at 3000 rpm for 30 minutes at low temperature. Take the supernatant, add 2g / L chitosan oligosaccharide at room temperature to form a precipitate, filter to remove the precipitate, adjust the pH value to 7.2 with NaOH, filter with a 0.22μm microporous membrane, and ultrafilter with a 10,000 molecular weight ultrafiltration membrane To obtain the liver hydrolyzed peptide solution, after measuring the polypeptide content, add 0.1g / liter of protective agent cystine, add a...

Embodiment 3

[0014] After removing the connective tissue, the bovine liver qualified for quarantine is mixed with water for injection at a weight ratio of 0.81:1, homogenized with a high-speed tissue grinder, heated to 50-51°C, and washed with Ca(OH) 2 Adjust the pH value to 7.0-7.5, add trypsin at 4g / liter, hydrolyze at 50-51°C for 1.5 hours, add A.T.3942 enzyme at 1g / liter, continue hydrolysis for 4 hours, adjust the pH value of the hydrolyzate to 6.0 with phosphoric acid, add 1g / L activated carbon, heat to boil, keep warm for 30 minutes, centrifuge at 3000rpm at low temperature for 30 minutes. Take the supernatant and add 2g / liter chitosan oligosaccharide at room temperature to form a precipitate, remove the precipitate by filtration to improve product purity, then adjust the pH value to 7.2 with NaOH, filter with a 0.22 μm microporous membrane, 10,000 molecular weight The liver hydrolyzed peptide solution is obtained by ultrafiltration with an ultrafiltration membrane. After measuring ...

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Abstract

A process for preparing the injection of hepatic hydrolyzed peptide features that the chitosan and oligose are used for adsorbing the modified protein and fat to improve the purity of product, and the cystine is used as the protector for increasing the sterilizing temp and time to 121 deg.C and 30 min, so having no bacterial pollution.

Description

technical field [0001] The invention relates to a pharmaceutical preparation for treating hepatitis and liver cirrhosis, in particular discloses a preparation method of hepatic hydrolyzed peptide injection, which belongs to the technical field of biochemical pharmacy. Background technique: [0002] Liver Hydrolyzed Peptide Injection is a sterile aqueous solution containing polypeptides, nucleic acids, and amino acids, which is extracted from the liver of healthy cattle and pigs by enzymatic hydrolysis. Hepatic hydrolyzed peptide can promote protein synthesis, reduce protein decomposition, promote the proliferation and regeneration of normal liver cells, have a good protective effect on liver cell damage induced by carbon tetrachloride, reduce alanine aminotransferase, and promote the recovery of diseased tissues. Hepatic hydrolyzed peptide injection is used for auxiliary treatment of chronic hepatitis, liver cirrhosis and other diseases. [0003] However, since liver hydrol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/01A61K35/407A61K9/08A61P1/16A61P31/14C12P21/00
Inventor 王巍
Owner 长春白求恩制药有限公司
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