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Preparation technology of liver hydrolytic peptide injection liquid

A technology for injection preparation and hepatic hydrolysis of peptides, which is applied to hydrolyzed protein components, medical preparations containing active ingredients, and the digestive system. Purity, the effect of ensuring drug safety

Inactive Publication Date: 2006-08-30
长春白求恩制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention discloses a preparation process of hepatic hydrolyzed peptide injection, which overcomes the disadvantage that the mold temperature of the existing preparation process cannot be too high, and the drug and the drug preparation are easy to "grow bacteria"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] After removing the connective tissue from the quarantined cattle liver, mix it with water for injection at a weight ratio of 0.80:1, homogenize it with a high-speed tissue masher, heat it to 51°C, and adjust the pH to 7.2 with CaOH. Add pancreatin at 4g / liter, hydrolyze at 50~51℃ for 1 hour, add AT3942 enzyme 1g / liter, continue hydrolysis for 4 hours, adjust the pH of the hydrolysate with phosphoric acid to 5.8~6.0, add 1g / liter activated carbon, heat and boil , Keep for 30 minutes, centrifuge at 3000rpm for 30 minutes at low temperature. Take the supernatant and add 2g / L chitosan oligosaccharide at room temperature to form a precipitate, filter to remove the precipitate, then adjust the pH to 7.2 with NaOH, filter with a 0.22um microporous filter membrane, 10,000 molecular weight ultrafiltration membrane The hepatic hydrolyzed peptide solution is filtered. After the peptide content is determined, 0.1g / L of cystine is added as a protective agent, and a certain amount (calcu...

Embodiment 2

[0012] After removing the connective tissue from the liver of a pig that has passed the quarantine, it is mixed with water for injection at a weight ratio of 0.82:1, homogenized with a tissue masher, heated to 51°C, adjusted to pH 7.0 with CaOH, and press 4g / L add pancreatin, hydrolyze at 50~51℃ for 1 hour, add AT3942 enzyme, continue hydrolysis for 5 hours, adjust the pH of the hydrolyzate to 5.8~6.0 with phosphoric acid, add 1g / L activated carbon, heat and boil, keep for 30 minutes , Centrifuge at 3000 rpm for 30 minutes at low temperature. Take the supernatant and add 2g / L chitosan oligosaccharide at room temperature to form a precipitate, filter to remove the precipitate, adjust the pH to 7.2 with NaOH, filter with a 0.22um microporous filter membrane, ultrafiltration with a 10,000 molecular weight ultrafiltration membrane Obtain the liver hydrolyzed peptide solution. After determining the peptide content, add 0.1g / L of protective agent cystine, dilute with a certain amount of...

Embodiment 3

[0014] After removing the connective tissue from the quarantined cattle liver, mix it with water for injection at a weight of 0.81:1, homogenize it with a high-speed tissue masher, heat it to 50~51℃, and adjust the PH value with CaOH 7.0~7.5, add pancreatin at 4g / liter, hydrolyze at 50~51℃ for 1.5 hours, add AT3942 enzyme at 1g / liter, continue hydrolysis for 4 hours, adjust the pH of the hydrolysate to 6.0 with phosphoric acid, add 1g / liter activated carbon , Heat and boil, keep for 30 minutes, centrifuge at 3000 rpm for 30 minutes at low temperature. Take the supernatant and add 2g / L chitosan oligosaccharide at room temperature to form a precipitate, filter to remove the precipitate to improve the purity of the product, then adjust the pH to 7.2 with NaOH, filter with a 0.22um microporous filter membrane, and a molecular weight of 10,000 Ultrafiltration membrane ultrafiltration to obtain liver hydrolyzed peptide solution. After determining the peptide content, add 0.1g / L of cysti...

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PUM

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Abstract

A process for preparing the injection of hepatic hydrolyzed peptide features that the chitosan and oligose are used for adsorbing the modified protein and fat to improve the purity of product, and the cystine is used as the protector for increasing the sterilizing temp and time to 121 deg.C and 30 min, so having no bacterial pollution.

Description

Technical field [0001] The invention relates to a pharmaceutical preparation for treating hepatitis and liver cirrhosis, and discloses a preparation process of a liver hydrolyzed peptide injection, which belongs to the technical field of biochemical pharmacy. Background technique: [0002] Hepatic hydrolyzed peptide injection is a sterile aqueous solution containing polypeptides, nucleic acids, and amino acids, which is prepared by enzymatic hydrolysis and extraction from the livers of healthy cattle and pigs. Hepatic hydrolyzed peptides can promote protein synthesis, reduce protein decomposition, promote the proliferation and regeneration of normal liver cells, have a better protective effect on carbon tetrachloride-induced liver cell damage, reduce alanine aminotransferase, and promote the recovery of diseased tissues. Hepatic hydrolyzed peptide injection is used for the auxiliary treatment of chronic hepatitis, cirrhosis and other diseases. [0003] However, because hepatic hy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K35/407A61K9/08A61P1/16A61P31/14C12P21/00
Inventor 王巍
Owner 长春白求恩制药有限公司
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