227results about How to "No risk of contamination" patented technology

A method of making a lithographic printing plate comprising the steps of: a) providing a lithographic printing plate precursor comprising (i) a support having a hydrophilic surface or which is provided with a hydrophilic layer, (ii) a photopolymerizable coating on said support, b) image-wise exposing said coating in a plate setter, c) developing the precursor, thereby removing the non-exposed areas of the coating from the support, whereby the developing step is carried out off-press in a gumming unit by treating the coating of the precursor with a gum solution.

A method of making a lithographic printing plate includes the steps of a) providing a lithographic printing plate precursor including (i) a support having a hydrophilic surface or which is provided with a hydrophilic layer, (ii) a coating on the support including a photopolymerizable layer, and, optionally, an intermediate layer between the photopolymerizable layer and the support, b) image-wise exposing the coating in a plate setter, c) optionally, heating the precursor in a preheating unit, and d) developing the precursor off-press in a gumming unit by treating the coating of the precursor with a gum solution, thereby removing the non-exposed areas of the coating from the support, wherein the coating further includes a compound capable of interacting with the support, the compound being present in the photopolymerizable layer and / or in the intermediate layer.

A vial for storing multiple doses of a substance to be dispensed into one or more syringes or other delivery devices. The vial has a body, a variable-volume storage chamber within the body for storing multiple doses of the substance therein, and a one-way valve connectable in fluid communication with a syringe or other delivery device. The one-way valve is moveable relative to the body between first and second positions (i) one of which permits the valve to open so that substance from the variable-volume storage chamber can flow therethrough and into the syringe or other delivery device connected in fluid communication therewith, and (ii) one of which prevents the valve from opening.

The invention relates to a device for sampling tissue from an animal, comprising sampling means provided with at least one cutting element (35) for cutting and collecting a sample of tissue from the animal, and means (31) for storing the sample, into which the cutting element is inserted once the sample has been collected. According to the invention, the storage means (31) have at least two openings, a first opening (32) for receiving the cutting element and the sample, and a second opening (33) cooperating with the detachable closing means (34).

The invention relates to a method for simultaneously detecting gene mutations of acetaldehyde dehydrogenase 2 and alcohol dehydrogenase 2. Concretely, a probe effective for detection of genetic polymorphism of the ALDH2 gene rs671 and the ADH2 gene rs1229984, a method for simultaneously detecting gene mutations and a kit for the purpose are provided. Thus the invention provides a probe for polymorphism detection, namely a probe for detection of at at least one type of genetic polymorphism of the ALDH2 gene rs671 and the ADH2 gene rs1229984, characterized in that: the probe comprises at least 1 type of fluorescently labeled oligonucleotide selected from (P1) to (P3') defined in the description.

Provided is a silicone gel composition including (A) an organopolysiloxane containing at least one alkenyl group bonded to a silicon atom within each molecule, represented by an average composition formula: Ra R1b SiO(4-a-b) / 2 (wherein, each R group represents, independently, an alkenyl group, each R1 group represents, independently, an unsubstituted or substituted monovalent hydrocarbon group that contains no aliphatic unsaturated bonds, a represents a positive number from 0.0001 to 0.2, b represents a positive number from 1.7 to 2.2, and a+b represents a number within a range from 1.9 to 2.4), (B) an organohydrogenpolysiloxane containing at least two hydrogen atoms bonded to silicon atoms within each molecule, and (C) a platinum-based catalyst. Also provided is the silicone gel composition stated above, in which a penetration value of a cured product of this composition, as defined in JIS K2220, is within a range from 20 to 200. The silicone gel composition generates a silicone gel cured product which displays a low elastic modulus and low stress, and suffers no oil bleeding over time.

A liquid medicament dispenser includes a housing and a pump carried by the housing, a reservoir applied to the housing and containing a pre-filled sterile liquid medicament, a sterile tubing set routed through the pump, a sterile administration line carried outside of the housing and coupled aseptically to the sterile tubing set in fluid communication, and an aseptic connector coupled to the reservoir and the sterile tubing set and changeable from a storage state to a use state. In the storage state, the aseptic connector prevents the liquid medicament in the reservoir from moving to the sterile tubing set. In the use state of the aseptic connector, the aseptic connector defines a sterile passageway for the flow of the liquid medicament from the reservoir to the sterile tubing set.

The invention relates to a method for storing integrity of nucleic acids (DNA and RNA) in a sputum sample in the field of biology. The invention provides phlegm paper, which can be used for conveniently storing nucleic acids in the sputum sample at room temperature; nucleic acid protecting liquid is infiltrated on the phlegm paper, and can be used for inactivating nuclease in the sputum sample; DNA and RNA in the sputum are effectively prevented from being degraded, so as to store and transport the sputum sample. At room temperature (25-35 DEG C), the nucleic acids in the sputum sample can be stably kept for 7-14 days. Meanwhile, the invention also provides a corresponding nucleic acid extraction method.

The invention relates to a printing apparatus for printing bottles or similar containers, having several printing positions on a transport element, which can be driven in rotation, by which the printing positions and / or the containers are moved on a closed path of movement between at least one container take-up and at least one container drop, having rotating print heads for applying at least one print image, preferably multi-color, onto a region to be printed on the external container surface of the containers, in the event of relative movement of the external container surface and at least one print head, wherein at least one enclosure is present, into which each of the containers, which are provided at a print position, is housed during the printing process with at least the region to be printed thereof.

The invention discloses a method for separating and culturing primary chicken hepatocytes. According to the method, EGTA (Ethylene Glycol Tetraacetic Acid) with low hepatotoxicity is added into a perfusate A to loosen the junction between hepatocytes, so that the dispersion degree of the to-be-separated hepatocytes is increased; double resistance is added into the perfusate A, a perfusate B and D-Hanks flushing fluid, so that the pollution is further prevented; and circulating perfusion digestion is adopted during digestion perfusion, so that the perfusion cost is greatly reduced. A serum-free cultural method of the chicken hepatocytes is established; compared with a traditional serum cultural method, the method disclosed by the invention has the same effect on the aspects of cell quantity, activity, function and the like, so that the problems caused by the existence of serum are solved and a foundation is laid for the substance metabolism study and biological product preparation on the basis of the chicken hepatocyte culture.

The invention discloses a preparation method and application of a classical swine fever virus recombinant subunit vaccine with the amino acid sequence shown as SEQ ID No.1. The preparation method of the classical swine fever virus recombinant subunit vaccine typically includes the following steps: classical swine fever E2 truncated protein (TE2) coding gene is cloned into baculovirus vector pFastBacTM1, and is then transfected into Sf9 insect cells to obtain recombinant baculovirus capable of expressing protein TE2. The high five insect cells in logarithmic growth phase are infected by the recombinant baculovirus, so that a large amount of the protein TE2 can be expressed in a cell culture supernatant. Finally, the cell culture supernatant is recovered and purified to obtain a large amount of the recombinant protein TE2 with the purity more than 90%. According to the method, the target protein can be harvested from the cell culture supernatant, the time of protein purification is reduced, consumption of a large amount of time can be avoided, and the vaccine production process can be simplified. Under the premise of simplification of the vaccine production process, the recombinant protein TE2 has the advantages of strong immunogenicity and high safety, and the animal experiments prove that the recombinant protein can effectively stimulate the body to produce a highly effective humoral immune response.

The invention discloses a method for preparing a precursor of a positive material of a nickel manganese cobalt multi-element lithium ion battery. The method comprises the following steps of: firstly, dissolving nickel, cobalt and manganese with deionized water, and preparing a multi-element metal salt solution with the total metal ionic molar concentration between 0.8 and 2.5mol / L, wherein the molar ratio of nickel to cobalt to manganese elements in the multi-element metal salt solution is (0-1):(0-1):(0-1); secondly, preparing alkali solution with concentration between 2 and 4mol / L; thirdly, reacting the salt solution with the alkali solution, wherein the temperature is controlled to be between 40 and 70 DEG C and the stirring speed is controlled to be between 100 and 600 revolutions / min, and the adding speed of salt and alkali solutions is controlled, so that the pH value of the reaction system is controlled to be between 8 and 11; and continuously stirring for 30 minutes to 2 hours after the reaction is completed, standing for 2 to 4 hours, filtering to obtain solid matters, and washing with deionized water until the content of Na<+> is lower than 0.03 percent, and drying to obtain the required precursor of the positive material of the nickel manganese cobalt multi-element lithium ion battery. According to the invention, an ammonia complexing agent is not used, and the method has the advantages of no ammonia or nitrogen pollution risk, good product sphericity, uniform granules, narrow granularity distribution and the like.

The invention discloses preparation methods and application of recombinant swine fever E2 protein and a subunit vaccine of the recombinant swine fever E2 protein. The preparation method of the recombinant swine fever E2 protein comprises the following steps that (1) a swine fever E2 protein coding gene is cloned into an eukaryotic expression vector to obtain recombinant plasmid containing the swine fever E2 protein coding gene; (2) then, the recombinant plasmid containing the swine fever E2 protein coding gene is transfected into a CHO cell strain; (3) the CHO cell strain obtained in the step(2) is cultured, screened and domesticated; and (4) the cell strain in the step (3) is fermented and cultured; and the recombinant swine fever E2 protein is obtained after purification. The methods provided by the invention have the advantages that the target protein can be obtained from cell culture supernatant; the yield reaches up to 1g / L; the protein purification time is shortened; the vaccineproduction steps are simplified; and the vaccine production cost is also greatly reduced.

The invention discloses a bar-shaped cigarette. The bar-shaped cigarette sequentially comprises a cigarette core material section (1), a cooling section (2) and a filter tip section (4), wherein the cooling section (2) is of a cylindrical bar-shaped structure, grooves (21) extending in the axial direction are formed in the outer wall of the cooling section (2), and openings of the grooves (21) face away from the axial center of the cooling section (2). The invention further discloses a preparation method of the bar-shaped cigarette. The cooling section (2) of the bar-shaped cigarette can effectively reduce the temperature of smoke gas and can further increase the amount of smoke to a certain extent at the same time; the used base materials and liquid additives can be quickly decomposed inthe nature without the risk of environmental pollution; and the loss in the manufacturing process is low, the preparation method is simple, and the manufacturing cost is reduced by more than 30%. In addition, the cooling section (2) can further be used in traditional cigarette filter tips to realize the addition of water-based essence and flavor filter tips.

The invention relates to a full-closed multi-index nucleic acid detection device. The device comprises a reagent cavity shell, an upper cover, a communicating vessel and a piston cylinder; a reagent cavity is formed in the reagent cavity shell, an upper cover is arranged on the reagent cavity shell, a sample adding hole is formed in the upper cover, the piston cylinder is arranged in the reagent cavity shell, a communicating hole is formed in a base plate of the piston cylinder, the communicating vessel is arranged below the piston cylinder, a communicating groove and a detection hole are formed in the communicating vessel, and an inner cavity of the piston cylinder can selectively communicate with a reagent cavity bottom hole by rotating the communicating hole; the reagent cavity comprises a sample pretreatment cavity, a lysis solution cavity, a magnetic bead cavity, a washing solution cavity and an eluate cavity, all the cavities are sealed cavities and can communicate with the innercavity of the piston cylinder through the communication hole and the communication groove, and the sample pretreatment cavity corresponds to the sample adding hole in position; and the detection holeonly communicates with the inner cavity of the piston cylinder and the reagent cavity through the communication hole. The device realizes full-sealing detection in the nucleic acid detection process,is convenient to operate and can realize simultaneous detection of multiple indexes of a single sample.

The system and method of the present invention includes an umbilical cord sampling device comprising a needle assembly, a base and at least one sampling needle operatively linked to at least one sampling reservoir. A removable cassette can be used to transfer a fluid to an analyzer to provide for the measurement of fluid characteristics.

The invention relates to a new process for controlling side wall inclination angle during manufacturing of array type high-voltage LED chips. The new process includes forming large-angle inclination on a side wall of a silicon dioxide mask by means of wet etching, then copying the large-angle inclination to an LED epitaxial layer, and accordingly forming an inclination side wall structure of micro-units of high-voltage LED chips.

The invention discloses an environment-friendly modular dredger. The dredger comprises a buoyant box module, a positioning pile system module, a bridge system module, a digging device module, a portal module, a supporting platform module, a power system module, a propelling system module, a living and control system module and a manipulation module. The environment-friendly modular dredger is convenient to transport by land and assemble and disassemble with the adoption of the standard functional modules and can operate after being transported to a designated lake and reservoir areas and assembled, and the standard modules can be used for series of vessels as required.

The invention belongs to the technical field of graphene preparation, and in particular relates to a graphene material based on a solid state mechanochemical reactor and a preparation method of the graphene material. According to the technical scheme of the invention, a three-dimensional strong shearing structure of the solid state mechanochemical reactor is utilized, a grinding aid is introduced in the graphite grinding process, the grinding aid and the graphite can be subjected to intense mutual friction caused by three-dimensional shearing force initialized by the three-dimensional shearing structure, then graphite can be peeled, and thus single-layer or small-layer graphene can be prepared. The preparation method comprises the following steps of: respectively drying graphite and the grinding aid, adding into a mixing machine, uniformly mixing, transferring into the solid state mechanochemical reactor for grinding, removing the grinding aid and impurity ions, and drying at 60-90 DEG C, thereby obtaining the high-performance graphene material of which the layer number is smaller than 8 and substrate defects are slightly caused. The novel preparation method of the graphene material provided by the invention has the characteristics of simplicity, high efficiency, cleanliness and low cost, the prepared graphene material has small substrate defects, and surface functionalization of graphene can be achieved at one step.

A device for treating a dental tissue comprising (a) an ozone generator for generating an ozone containing gas, (b) a first pump in fluid flow communication with the ozone generator for generating a flow of ozone containing gas, (c) an applicator for multiple teeth having a surface suitable for forming with the hard and soft dental tissue of multiple teeth to be treated (d) a treatment chamber having an inlet orifice in fluid flow communication with the ozone generator and the first pump and having an outlet orifice, and (e) a second pump in fluid flow communication with the outlet orifice of the applicator for removing gas from the treatment chamber. The second pump has a second throughput and the first pump has a first throughput whereby the ratio of the second throughput to the first throughput is at least 1.5:1.

The present invention relates to an apparatus for performing heat treatment of a bodily cavity in a mammal. The apparatus comprises a catheter intended for insertion into said bodily cavity, an inflatable balloon surrounding a distal end of said catheter in a liquid tight manner which balloon is adapted in use of said apparatus to be inflated and dilated in said bodily cavity, control means operatively connected to said balloon by at least one oscillation lumen and being provided with an oscillation assembly adapted to drive a heat transmitting fluid within said apparatus, and heating means adapted to heat said heat transmitting fluid. Said control means are adapted to control pressure and temperature of said heat transmitting fluid. The heating means have a heat emitting portion and a heat receiving portion which is use of the apparatus are positioned in thermal contact with each other, and which are separable from each other.

Process for distillatively separating a mixture containing a vinyl ether of the general formula (I)R1—O—CH═CH2  (I)and alcohol of the general formula (II)R2—OH  (II)in which R1 and R2 are each independently a saturated or unsaturated, aliphatic or cycloaliphatic radical having from 2 to 10 carbon atoms, and in which the alcohol (II) has a boiling point which is at least 1° C. higher, measured at or extrapolated to 0.1 MPa abs, than the vinyl ether (I), bya) passing the mixture into a first distillation column and withdrawing, as a top product, an azeotrope containing vinyl ether (I) and alcohol (II) and, as a bottom product, a stream enriched with the alcohol (II);b) passing the azeotrope containing vinyl ether (I) and alcohol (II) from the first distillation column into a second distillation column which is operated at a pressure which is from 0.01 to 3 MPa higher compared to the first distillation column, and withdrawing, as a bottom product or gaseous sidestream in the stripping section, the vinyl ether (I) and, as a top product, an azeotrope containing vinyl ether (I) and alcohol (II); andc) recycling the azeotrope containing vinyl ether (I) and alcohol (II) from the second distillation column into the first distillation column.

The invention provides an electroslag remelting device and an electroslag remelting method for refining metal scraps. The electroslag remelting device at least comprises a water-cooled crystallizer, an electrode, a bottom water tank, a power control cabinet and cables, wherein the water-cooled crystallizer is placed on the bottom water tank; a vibrating feeder for adding the metal scraps is arranged at the top of the water-cooled crystallizer; the electrode is a water-cooled electrode; the water-cooled electrode is mounted on a lifting cross arm; one end of the lifting cross arm is connected with an upright column, and can rise and fall along the upright column; the water-cooled electrode is inserted into the water-cooled crystallizer in a centering manner; the lifting cross arm and the bottom water tank are connected with the power control cabinet through the cables respectively. The electroslag remelting device is simple in structure and convenient to use, can effectively refine themetal scraps, does not bring refractory pollution, and does not cause an increase in the carbon content.

The system and method of the present invention includes an umbilical cord sampling device comprising a needle assembly, a base and at least one sampling needle operatively linked to a removable cassette containing at least one sampling reservoir.

The invention relates to a deep scrap iron catalytic ozonation wastewater treatment method. The deep scrap iron catalytic ozonation wastewater treatment method is characterized in that (1) a passivation layer is formed on surface of scrap iron through chemical modification, wherein main components of the passivation layer are Fe(II) and Fe(III) oxides, so that a heterocatalysis oxidation mechanism to O3 is formed; (2) a certain amount of ion-state Fe<2+> and Fe<3+> are formed by virtue of scrap iron not subjected to surface modification during oxidation of O3, so that an in-phase catalysis oxidation mechanism to O3 is formed; and (3) a certain amount of iron ions are also produced when the surface modified iron ions are used as a catalyst. The surface modified iron ions can be taken as a coagulate flocculating agent, and a coagulant aid is added to form a coagulating sedimentation or contact filtration mechanism, so that the iron ions are removed, and organic matters in the water can be further removed. During catalytic ozonation of the prepared catalyst, COD removal rate of secondary biochemical treated effluent of general industrial wastewater is 50-80%, and the new national discharge standard requirement can be completely met, so that application value is not influenced.

A fast recovery diode includes an n-doped base layer having a cathode side and an anode side opposite the cathode side. A p-doped anode layer is arranged on the anode side. The anode layer has a doping profile and includes at least two sublayers. A first one of the sublayers has a first maximum doping concentration, which is between 2*1016 cm−3 and 2*1017 cm−3 and which is higher than the maximum doping concentration of any other sublayer. A last one of the sublayers has a last sublayer depth, which is larger than any other sublayer depth. The last sublayer depth is between 90 to 120 μm. The doping profile of the anode layer declines such that a doping concentration in a range of 5*1014 cm−3 and 1*1015 cm−3 is reached between a first depth, which is at least 20 μm, and a second depth, which is at maximum 50 μm. Such a profile of the doping concentration is achieved by using aluminium diffused layers as the at least two sublayers.

The invention discloses a synthesis method of guanidine organic magnetic ionic liquid, in particular guanidyl cation-based organic magnetic ionic liquid. The synthesis method is technically characterized in that chloroalkane or bromoalkane, 1,1,3,3-tetramethyl guanidine, sodium hydroxide or potassium hydroxide, a 4-hydroxy-2,2,6,6-tetramethyl piperidine oxide and chlorosulfonic acid are used as raw materials to synthesize a series of paramagnetic organic magnetic ionic liquid, and the structural formula is as shown in formula (I) shown in the description. The compound is novel in structure and can form aqueous two phase with inorganic salt (potassium phosphate, dipotassium phosphate, potassium carbonate, sodium carbonate, ammonium bisulfate, sodium sulfate and the like); an aqueous two phase system of the magnetic ionic liquid is internationally developed for the first time. Compared with the traditional aqueous two phase system, the aqueous two phase system of the magnetic ionic liquid is capable of accelerating the separation of two phases through an applied magnetic field assisted manner, so as to improve the phase separation efficiency. The system has wide potential purposes, and has unique advantage in the fields of extraction, micro-extraction, separation and analysis.

The invention relates to a passive valve for multi-valve devices for sealing a container or a line. According to one aspect of the invention, the passive valve is free of bearing supports which protrude in a positive sense out of the passive valve. The invention also relates to a multi-valve device for contamination-free connection of two containers or two lines with partial valves that can be rotated about a single axis. According to this aspect of the invention, at least three partial valves, which are arranged essentially parallel to one another and can be put under tension so they can be sealed with respect to their surroundings, can be rotated about the single shared axis.