Full-closed multi-index nucleic acid detection device

Abstract

The invention relates to a full-closed multi-index nucleic acid detection device. The device comprises a reagent cavity shell, an upper cover, a communicating vessel and a piston cylinder; a reagent cavity is formed in the reagent cavity shell, an upper cover is arranged on the reagent cavity shell, a sample adding hole is formed in the upper cover, the piston cylinder is arranged in the reagent cavity shell, a communicating hole is formed in a base plate of the piston cylinder, the communicating vessel is arranged below the piston cylinder, a communicating groove and a detection hole are formed in the communicating vessel, and an inner cavity of the piston cylinder can selectively communicate with a reagent cavity bottom hole by rotating the communicating hole; the reagent cavity comprises a sample pretreatment cavity, a lysis solution cavity, a magnetic bead cavity, a washing solution cavity and an eluate cavity, all the cavities are sealed cavities and can communicate with the innercavity of the piston cylinder through the communication hole and the communication groove, and the sample pretreatment cavity corresponds to the sample adding hole in position; and the detection holeonly communicates with the inner cavity of the piston cylinder and the reagent cavity through the communication hole. The device realizes full-sealing detection in the nucleic acid detection process,is convenient to operate and can realize simultaneous detection of multiple indexes of a single sample.

Description

technical field [0001] The invention relates to a fully-sealed multi-indicator nucleic acid detection device, in particular to a detection device integrating sample pretreatment, nucleic acid extraction, nucleic acid amplification and detection. Background technique [0002] Nucleic acid detection requires the following steps: sample pretreatment, nucleic acid extraction, reagent preparation, amplification and detection. At present, nucleic acid detection has become a common detection method, but at present, the above steps of nucleic acid detection are mostly manual operations, or Part of the process is completed with the help of semi-automatic instruments. The entire process not only requires professional technicians, but also requires a professional laboratory, especially for samples with high hazards, which need to be carried out in a P2-level biosafety laboratory, which further limits nucleic acid. The promotion and application of detection technology. At present, ther...

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Problems solved by technology

[0002] Nucleic acid detection requires the following steps: sample pretreatment, nucleic acid extraction, reagent preparation, amplification and detection. At present, nucleic acid detection has become a common detection method, but at present, the above steps of nucleic acid detection are mostly manual operations, or Part of the process is completed with the help of semi-automatic instruments. The entire process not only requires professional technicians, but also requires a professional laboratory, especially for samples with high hazards, which need to be carried out in a P2-level biosafety laboratory, which further limits nucleic acid. Promotion and application of detection technology

At present, there are fully automatic detection instruments on the market, but there are still problems such as the need for sample pretreatment, complicated operation, high cost, limited detection indicators and the inability to realize multi-index screening, etc., which limit a wide range of applications.

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