13 results about "Aqueous two-phase system" patented technology

Provided herein are methods of isolating extracellular vesicles (EVs) and kits for use with the methods disclosed herein. The methods include, at a first step, subjecting the sample to one or more cycles of phase separation using an aqueous two-phase system (ATPS). The ATPS includes a first phase comprising a first-phase polymer and a second phase comprising a second-phase polymer. The EVs are enriched in the first phase. The methods also include, at a second step, treating the first phase with the enriched EVs with one or more enzymes that degrades the first-phase polymer.

The invention discloses a method for enriching phenolic acid and flavonoid compounds in perilla leaves. The method comprises the following steps: firstly, adding perilla leaf powder, NaH2PO4 and lauryl sodium sulfate into a [C6MIM] Br solution, adjusting the pH value to 7.4-7.6, carrying out vortex treatment at room temperature for 120-160s, and carrying out centrifugal treatment to form a stable aqueous two-phase system; taking an upper-layer enrichment phase of the aqueous two-phase system, diluting with methanol, carrying out centrifugal treatment, taking supernate, filling the supernate into a small liquid-phase bottle, and carrying out UHPLC (Ultra High Performance Liquid Chromatography) analysis to obtain a perilla leaf chromatogram; protocatechuic aldehyde, caffeic acid, luteoloside, rosmarinic acid and luteolinin are equivalently added into methanol, UHPLC analysis is carried out, and a standard chromatogram is obtained; and carrying out quantitative analysis on phenolic acid and flavonoid substances in the perilla leaves according to the chromatogram of the perilla leaves and the chromatogram of the standard substance. The method is suitable for one-time extraction and enrichment of phenolic acid and flavonoid compounds in a solid matrix, and consumption of samples and reagents is reduced.

Disclosed herein are methods of isolating and concentrating viruses from a sample using two aqueous two-phase systems (ATPSs). Also disclosed herein is a system comprising a first ATPS and a second ATPS.

The present disclosure relates to methods, compositions and kits for concentrating and purifying one or more target analytes from a large number of fluid samples. In some embodiments, the methods involve at least two first aqueous two-phase system (ATPS) compositions. Some embodiments provide kits comprising at least two ATPS compositions and a binding buffer. Other embodiments provide methods of treating bladder cancer in a patient in need thereof.

More specifically, the method can efficiently extract and concentrate regeneration factor-rich plasma (RRP) having a regeneration effect in a short time, and the RRP produced using the method promotes cell proliferation, making it suitable for regeneration therapy and skin beauty.

This invention discloses a method for preparing multifunctional sensing hollow gel fibers based on an aqueous two-phase system. The technical solution is based on an aqueous two-phase system formed by polyvinyl alcohol (PVA) and dextran in water. Using a coaxial spinning process, a PVA solution is injected into the shell channel of a coaxial spinning device, and a dextran solution is injected into the core channel. Relying on the ultra-low tension between the two aqueous phase interfaces, the PVA solution and dextran solution after extrusion are kept in a parallel laminar flow state. After coagulation treatment, a core-shell structure gel fiber is obtained. Subsequently, it undergoes solvent evaporation treatment, water washing treatment, and eutectic solvent replacement to finally prepare the hollow gel fiber. The gel fiber of this invention possesses stretchability, compression resilience, high conductivity, and a wide operating temperature range. It can be made into multidimensional sensors and humidity sensors to monitor strain, direction, pressure, and humidity, exhibiting considerable sensitivity and stability.

The invention discloses a method for driving liquid drops to move in an aqueous two-phase system and application of the method, and the method comprises the following steps: dissolving polyethylene glycol and glucan in deionized water, centrifuging and standing for layering to respectively obtain a PEG solution and a DEX solution; a DEX solution is dropwise added to different positions of the PEG solution containing SDBS, and two DEX liquid drops are formed; dropwise adding a DEX solution containing calcium chloride between the two DEX liquid drops to form DEX liquid drops containing calcium chloride; the two DEX liquid drops can move towards the DEX liquid drop containing calcium chloride and are finally gathered together; or dropwise adding a DEX solution containing calcium chloride to different positions of the PEG solution containing SDBS to form two DEX liquid drops containing calcium chloride; the two DEX liquid drops containing calcium chloride can move towards each other and finally gather together. The method can break through the limitation of low interfacial tension of an aqueous two-phase system, realizes controllable movement of liquid drops, has biocompatibility, and can be used for conveying biochemical reagents and initiating an enzymatic reaction.

Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.

The present invention relates to a cosmetic composition containing iris bulb-derived exosomes as an effective ingredient, and more particularly to a cosmetic composition containing iris bulb-derived exosomes purified by ultrahigh pressure pretreatment and a two-aqueous phase system as an effective ingredient, which has excellent stability and excellent skin moisturizing, skin wrinkle improving and skin whitening effects.

The invention discloses a preparation method of undaria pinnatifida protein with antioxidant activity, which comprises the following steps: firstly, effectively destroying the cell wall structure of undaria pinnatifida by using a compound enzyme to fully release protein in cells, and further obtaining undaria pinnatifida crude protein by using acid-base treatment and an isoelectric precipitation method; and separating and purifying the extracted crude protein through a PEG / (NH4) 2SO4 aqueous two-phase system. According to the method, a polyethylene glycol (PEG) / (NH4) 2SO4 aqueous two-phase system is adopted, so that polyphenol, pigment and other impurities in the undaria pinnatifida protein can be effectively removed, and the purity of the undaria pinnatifida protein is effectively improved; and step-by-step acid and alkali treatment is adopted, so that the influence of algin precipitation on the protein can be avoided, the extraction rate of the protein is effectively improved, the undaria pinnatifida protein with high purity and strong antioxidant activity is further obtained, and the undaria pinnatifida protein has relatively high commercial value. Meanwhile, the method has the advantages of simple steps, easiness in operation and the like, and is suitable for industrial large-scale production.

The present disclosure relates to methods, compositions and kits for isolating a target nucleic acid below a target size from a sample comprising nucleic acid components. In some embodiments, the methods involve one or more aqueous two phase system (ATPS) compositions, at least one solid phase medium, and at least one buffer. Some embodiments provide kits comprising one or more ATPS compositions, at least one solid phase medium, and at least one buffer. Other embodiments provide methods of diagnosing a disease or condition using the methods described herein.

In a hydrogel preparation process, if a biological substance sensitive to external reaction is added, physical or chemical stimulation applied in a traditional hydrogel preparation method not only needs a complex process, but also possibly affects the activity and structure of particles in the hydrogel. On the other hand, according to the hydrogel utilizing the aqueous two-phase system and the preparation method of the hydrogel, provided by the invention, the formation of the hydrogel and the encapsulation of the target nanoparticles in the hydrogel can be realized at the same time. The present invention successfully and simply concentrates target nanoparticles in a hydrogel in a high yield. Furthermore, the present invention relates to a hydrogel using an aqueous two-phase system and a method for preparing the same, in which concentration and hydrogel formation are performed at the same time, thereby shortening the preparation time of the hydrogel and significantly reducing the cost, by promoting the synchronization of concentration and hydrogel, and to a method for preparing the hydrogel using the aqueous two-phase system.

The invention relates to an exosome extraction and purification composition and a preparation method thereof, the exosome extraction and purification composition mainly comprises the following substances: polyethylene glycol PEG and glucan, the polyethylene glycol PEG and the glucan form an incompatible aqueous two-phase system in an aqueous solution, the exosome is selectively distributed to an upper phase containing the polyethylene glycol PEG, impurities are dissolved in a lower phase containing the glucan, and the exosome extraction and purification composition is obtained. Therefore, high recovery rate and high purity extraction of the exosome are realized. According to the exosome extraction and purification combination and the preparation method thereof, the cost is low, and the recovery rate of the peripheral blood exosome can be remarkably increased.