Polyethylene glycol/glucan aqueous two-phase system emulsion stabilizer and preparation method thereof

Abstract

The invention discloses a polyethylene glycol / glucan aqueous two-phase system emulsion stabilizer and a preparation method thereof. The preparation method comprises the steps: adding mPEG-CHO (methoxy polyethylene glycol aldehyde) to a protein aqueous solution, and fully stirring until being dissolved, wherein the molar ratio of a protein to mPEG-CHO is 1:10-1:13; then adding a certain amount of dilute hydrochloric acid into the aqueous solution to adjust the pH value to be slightly higher than a protein isoelectric point, carrying out full reaction for 20 h at a constant temperature of 0-4 DEG C, then precipitating, and drying to obtain the target product. The method is simple and convenient, mild in preparation conditions and easy to control, and has fewer side reactions. Because the surface of the product has moderate wettability with two phases of both polyethylene glycol and glucan, the product is adsorbed on a two-phase interface, enables the stable aqueous two-phase emulsion effect to be achieved by changing the interfacial tension, can retain the biological activity of the protein to a larger extent, can achieve aqueous two-phase emulsion loading of bioactive substances, and has good application prospects in the fields of foods, cosmetics and drug controllable release.

Description

technical field [0001] The invention relates to the field of fine chemical industry, especially the preparation of two-phase emulsion stabilizer. Background technique [0002] The traditional emulsion preparation process needs to add a large amount of surfactants and organic solvents, so when the emulsion product functions, a large amount of surfactants and organic solvents will be discharged into the environment, causing environmental pollution. Many industries have begun to call for the emergence of new emulsions with short dissolution time, convenient use, and environmental protection. The emergence of water-in-water emulsions solves the above problems. The preparation process of water-in-water emulsions does not need to add any organic solvents, and has good solubility and dispersibility. , easy to use, green and environmentally friendly, and high biocompatibility. However, water-in-water emulsions have disadvantages such as difficult milk formation and poor stability, ...

AI Technical Summary

Problems solved by technology

However, the protein particles used to stabilize aqueous two-phase emulsions are not natural proteins, but prepared by heating protein solutions, and the protein particles have been denatured and lost their biological activity; triblock copolymers used to stabilize aqueous two-phase emulsions It is produced by the copolymerization of three different polymers. The production cost of the tri-block copolymer is high, the preparation process is relatively complicated, and the block does not contain biologically active substances

Since the research in this field is in its infancy, there are too few methods for stabilizing two-phase emulsions, narrow application ranges, and products that can impart biological activity to two-phase emulsion stabilizers have not been reported, so the market is in urgent need of a new type of two-phase emulsion. Phase emulsion emulsifier, which can not only stabilize water-in-water emulsion, but also expand the application of water-in-water emulsion in loading biological active substances