Rhodiola rosea fermentation protoplasm cosmetic and preparation method thereof
A technology for fermented puree and cosmetics, which is applied to the field of Rhodiola fermented puree cosmetics and preparation thereof, can solve the problems of inability to fully exert the beauty effect of Rhodiola rosea, limited extract components, and limited extraction conditions, and achieves maintaining natural activity. , The effect of avoiding the loss of active ingredients and ensuring safety
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Embodiment 1
[0034] Embodiment 1, preparation and detection of Rhodiola rosea fermented puree cosmetics
[0035] The concentration of fermentation bacteria in the expansion culture medium was 10 5 -10 8 Inoculate 20mL of CFU / ml fermented bacteria liquid into 50 mesh 10g rhodiola root powder and 200g water, and ferment in a shaker at 28°C for 48 hours, then autoclave the obtained fermentation liquid at 115°C for 20min Autoclave to inactivate the bacteria, centrifuge the sterilized fermented liquid for 20 min at 12,000 r / min and a centrifugal radius of 9 cm, collect the supernatant, which is the rhodiola fermented puree cosmetic provided by the present invention.
[0036] The appearance of the Rhodiola rosea fermented puree cosmetic prepared in this example is viscous liquid, and the color is light pink to brownish red. The pH value is 5.2-6.8, the viscosity is 200-500cP, the soluble solid content is 1.5-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria a...
Embodiment 2
[0039] Embodiment 2, the antioxidation effect analysis of Rhodiola rosea fermented protoplasm
[0040] DPPH is an early synthesized organic free radical, which is often used to evaluate the hydrogen-donating ability of antioxidants. It is very stable in organic solvents, has a purple color, and has a characteristic absorption peak at , when it encounters a free radical scavenger , the lone pair of electrons of DPPH is paired to make it fade, that is, the light absorption value at the maximum absorption wavelength becomes smaller. Therefore, the scavenging effect of the sample on DPPH free radicals can be evaluated by measuring the change of the absorbance value.
[0041] The specific experimental steps of the DPPH free radical scavenging experiment are as follows:
[0042] (1) Take an equal volume (usually 3mL) of the solution to be tested and 2×10 -4 mol / L DPPH solution mixed (A 1 Tube);
[0043] (2) Take an equal volume of absolute ethanol (the solvent for the analyte) a...
Embodiment 3
[0049] Embodiment 3, the whitening efficacy analysis of Rhodiola rosea fermented protoplasm
[0050] Tyrosinase is the key enzyme of melanin production, it controls the formation process of melanin, and its activity plays a major role in the deposition of pigment. Many whitening and freckle-removing products currently on the market achieve whitening effects by inhibiting tyrosinase, so the strength of the inhibitory effect on tyrosinase is the main indicator for evaluating whitening cosmetics.
[0051] Evaluate the whitening function of the sample by measuring the impact of the sample on tyrosinase, the specific method is as follows:
[0052] Configure the solution according to Table 1:
[0053] Table 1, solution preparation list
[0054] Unit (mL)
C 1
C 2
T 1
T 2
L-tyrosine
2
2
2
2
sample
0
0
2
2
PBS
4
5
2
3
1
0
1
0
total capacity
7
...
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