Method for preparing gelsolin from Dendrorhynchus zhejiangensis
A technology of gelsolin and chitosan, which is applied in the field of preparing gelsolin, can solve rare problems and the like, achieves the effect of eliminating renaturation steps, maintaining natural activity, and being easy to popularize
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[0017] The method for preparing gelsolin from Nematodes zhejiang, comprises the following steps:
[0018]a. RNA extraction: Clean C. zhejiangensis to obtain C. zhejiangensis body wall, quickly freeze the C. zhejiangensis body wall in liquid nitrogen, grind into powder, and then extract total RNA with RNAiso plus RNA extraction kit, The specific method is operated according to the kit instructions: after the body wall tissue is ground into powder, weigh 5 grams, add 100ml of RNAiso lysate, homogenate, let stand at room temperature for 5 minutes, and centrifuge at 4°C for 5 minutes at 12,000 rpm / min. Take the first supernatant, add chloroform, the volume ratio of the first supernatant to chloroform is 5:1, after the solution is fully emulsified, let stand at room temperature for 5 minutes, centrifuge at 12000 rpm / min at 4°C for 15 minutes, and take Add isopropanol to the second supernatant, the volume ratio of the second supernatant to isopropanol is 1:1, let stand at room tempe...
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