Method for rapidly detecting contents of lemon yellow and sunset yellow in food
A tartrazine and sunset yellow technology, used in measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult to purify samples, secondary pollution of solvents, low enrichment efficiency, etc., and achieve short separation time and short phase separation time. , the effect of good liquidity
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Embodiment 1
[0018] Embodiment 1, a kind of method for quickly detecting tartrazine and sunset yellow content in food, the steps of this method are as follows:
[0019] (1) Preparation of working curve: Prepare a series of tartrazine and sunset yellow standard solutions with a concentration of 500 mg / mL and a solution concentration in the range of 0.05 μg / mL to 50.0 μg / mL by stepwise dilution method Yellow standard solution; use high performance liquid chromatography to measure the peak areas of the above various concentrations of standard solutions, draw a standard curve, and obtain a linear regression equation. The results of the measurement are shown in Table 1.
[0020] Table 1 Method parameters
[0021] Analyte Extraction rate linear regression equation Linear range (μg / mL) Detection limit (μg / mL) The relative standard deviation sunset yellow 98.91% Y=707.74x(μg / mL)+56.03(480nm) 0.05-50.0 0.011 0.80 lemon yellow 99.24% Y=264.46x(μg / mL)+21.24(410nm...
Embodiment 2
[0027] Embodiment 2, a kind of method for fast detection tartrazine content in large yellow croaker, the steps of this method are as follows:
[0028] 1. Making the working curve: the method is the same as the method in Step 1 of Example 1.
[0029] 2. Sample processing:
[0030] Wash the large yellow croaker body dyed with lemon yellow, add distilled water for ultrasonic extraction once, the extraction time is 10-30 minutes, and the extraction temperature is 30°C; after the extraction is completed, it is filtered through a 0.45 μm microporous membrane and directly injected into the sampling bottle. The test solution of large yellow croaker was obtained;
[0031] Take 3.00ml large yellow croaker test solution in a 10mL stoppered centrifuge tube, add 0.3mL 1-butyl-3-methylimidazolium bromide, dilute to 4mL with distilled water, add 3.5g K 2 HPO 4 , shake until completely dissolved, centrifuge at 2000-3000r / min for 2-3min to form a two-water phase, take 20 μL of the ionic liq...
Embodiment 3
[0032] Embodiment 3, a kind of method for quickly detecting tartrazine and sunset yellow content in orange-flavored carbonated drinks, the steps of this method are as follows:
[0033] 1. Making the working curve: the method is the same as the method in Step 1 of Example 1.
[0034] 2. Sample processing
[0035] Take 3.00mL of a certain brand of orange-flavored beverage in a 10mL stoppered centrifuge tube, and heat it ultrasonically for 10min to remove CO 2 , add 0.3mL, 1-butyl-3-methylimidazolium bromide, dilute to 4mL mark with distilled water, add 3.5g K 2 HPO 4 , shake until completely dissolved, centrifuge at 2000-3000r / min for 2-3min to form a two-water phase, take 20 μL of the ionic liquid phase, and directly perform chromatographic analysis. According to the working curve obtained in Table 1, the The contents of tartrazine and sunset yellow were 1.46μg / g and 17.68μg / g, respectively.
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