Method for rapidly detecting contents of lemon yellow and sunset yellow in food

A tartrazine and sunset yellow technology, used in measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult to purify samples, secondary pollution of solvents, low enrichment efficiency, etc., and achieve short separation time and short phase separation time. , the effect of good liquidity

Inactive Publication Date: 2013-09-18
JIANGSU MARINE RESOURCES DEV RES INST LIAN YUNGANG +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Volatile organic compounds are often used in the pretreatment process of traditional analytical samples. At the same time, there are problems such as low enrichment...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, a kind of method for quickly detecting tartrazine and sunset yellow content in food, the steps of this method are as follows:

[0019] (1) Preparation of working curve: Prepare a series of tartrazine and sunset yellow standard solutions with a concentration of 500 mg / mL and a solution concentration in the range of 0.05 μg / mL to 50.0 μg / mL by stepwise dilution method Yellow standard solution; use high performance liquid chromatography to measure the peak areas of the above various concentrations of standard solutions, draw a standard curve, and obtain a linear regression equation. The results of the measurement are shown in Table 1.

[0020] Table 1 Method parameters

[0021] Analyte Extraction rate linear regression equation Linear range (μg / mL) Detection limit (μg / mL) The relative standard deviation sunset yellow 98.91% Y=707.74x(μg / mL)+56.03(480nm) 0.05-50.0 0.011 0.80 lemon yellow 99.24% Y=264.46x(μg / m...

Embodiment 2

[0027] Embodiment 2, a kind of method for fast detection tartrazine content in large yellow croaker, the steps of this method are as follows:

[0028] 1. Making the working curve: the method is the same as the method in Step 1 of Example 1.

[0029] 2. Sample processing:

[0030] Wash the large yellow croaker body dyed with lemon yellow, add distilled water for ultrasonic extraction once, the extraction time is 10-30 minutes, and the extraction temperature is 30°C; after the extraction is completed, it is filtered through a 0.45 μm microporous membrane and directly injected into the sampling bottle. The test solution of large yellow croaker was obtained;

[0031] Take 3.00ml large yellow croaker test solution in a 10mL stoppered centrifuge tube, add 0.3mL 1-butyl-3-methylimidazolium bromide, dilute to 4mL with distilled water, add 3.5g K 2 HPO 4 , shake until completely dissolved, centrifuge at 2000-3000r / min for 2-3min to form a two-water phase, take 20 μL of the ionic l...

Embodiment 3

[0032] Embodiment 3, a kind of method for quickly detecting tartrazine and sunset yellow content in orange-flavored carbonated drinks, the steps of this method are as follows:

[0033] 1. Making the working curve: the method is the same as the method in Step 1 of Example 1.

[0034] 2. Sample processing

[0035] Take 3.00mL of a certain brand of orange-flavored beverage in a 10mL stoppered centrifuge tube, and heat it ultrasonically for 10min to remove CO 2 , add 0.3mL, 1-butyl-3-methylimidazolium bromide, dilute to 4mL mark with distilled water, add 3.5g K 2 HPO 4 , shake until completely dissolved, centrifuge at 2000-3000r / min for 2-3min to form a two-water phase, take 20 μL of the ionic liquid phase, and directly perform chromatographic analysis. According to the working curve obtained in Table 1, the The contents of tartrazine and sunset yellow were 1.46μg / g and 17.68μg / g, respectively.

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Abstract

The invention discloses a method for rapidly detecting contents of lemon yellow and sunset yellow in a food. The method comprises the following steps of: preparing a standard solution of the lemon yellow and the sunset yellow; measuring the peak area of the standard solution of the pigments by using high performance liquid chromatography, and drawing a standard curve to obtain an equation of linear regression; filtering a liquid food sample or solid food sample solution, extracting by using an ionic liquid aqueous two-phase system composed of 1-butyl-3-methylimidzaole bromine salt and K2HPO4, and directly carrying out high performance liquid chromatography analysis on an ionic liquid after phase splitting; and respectively calculating the contents of the lemon yellow and the sunset yellow in the food according to the equation of linear regression. The method disclosed by the invention is simple in operation, free of a great deal of volatile solvents, short in separation time, good in ionic liquid phase mobility and capable of inter-dissolving with water or methanol and directly injecting for chromatography analysis. By using the method, the rapid, simple, convenient, environment-friendly, economic, qualitative and quantitative measurement can be provided for quality control departments and production enterprises.

Description

technical field [0001] The invention relates to a method for determining content by high-performance liquid chromatography, in particular to a method for quickly detecting the content of tartrazine and sunset yellow in food, and belongs to the technical field of food safety additive detection. Background technique [0002] Due to the wanton discharge of domestic wastewater rich in elements such as carbon, nitrogen, and phosphorus into natural water bodies, these elements can eventually lead to eutrophication of water bodies through enrichment effects. At present, there have been a lot of researches on the treatment of domestic and municipal wastewater. Among them, the research on the removal of phosphorus started relatively late, and it was not started until the 1980s and 1990s. Lemon yellow, a water-soluble synthetic pigment, bright bright yellow. Sunset yellow is also known as sunset yellow, sunset yellow, orange, and edible yellow No. 3. These two pigments can be safel...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 沙鸥马卫兴陈丽丁军楠马晓冬赵旭兰
Owner JIANGSU MARINE RESOURCES DEV RES INST LIAN YUNGANG
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