18results about How to "Short separation time" patented technology

The utility model discloses a full-automatic whole blood sample pretreatment device for separating plasma through immunomagnetic beads. The full-automatic whole blood sample pretreatment device comprises a plasma separation mechanism for extracting a plasma sample from a whole blood sample, a conveying mechanism for conveying a blood collection tube and a sample cup, and a feeding mechanism for providing the sample cup, the plasma separation mechanism comprises a sample loading and shaking module for uniformly mixing a whole blood sample in the blood collection tube, a puncture sampling module for collecting the whole blood sample or the plasma sample, and a magnetic separation module for separating the plasma sample; the magnetic separation module comprises an oscillation assembly, a magnetic attraction assembly and a reaction cup, and freeze-dried immunomagnetic beads and an antibody reagent are pre-stored in the reaction cup. The device provided by the utility model can be used for comprehensively and automatically pretreating a whole blood sample and improving the quality and the efficiency of plasma separation.

The invention discloses a carboxyl-terminated hyperbranched magnetic silver-based catalyst as well as a preparation method and application thereof, and relates to the technical field of wastewater treatment. Comprising the following steps: preparing magnetic Fe3O4 nanoparticles MNP by adopting a coprecipitation method; the preparation method comprises the following steps: sequentially coating the surface of MNP with SiO2 and gamma-aminopropyltrimethoxysilane, and synthesizing an aminated magnetic nanoparticle FSN; the preparation method comprises the following steps: carrying out blending reaction on FSN and a carboxyl-terminated hyperbranched polymer solution, washing, separating a product by a magnet, and drying to obtain carboxyl-terminated hyperbranched magnetic silicon shell particles FSNC; fSNC is dissolved in a silver nitrate solution for reaction, then sodium borohydride is added for continuous reaction, washing, product separation by a magnet and drying are performed, and the carboxyl-terminated hyperbranched magnetic silver-based catalyst Ag / FSNC is obtained. The carboxyl-terminated hyperbranched magnetic silver-based catalyst provided by the invention can effectively catalyze reduction of p-nitrophenol into p-aminophenol, has good magnetic responsiveness, is convenient for recovery and reuse of the catalyst, can continue a catalytic experiment without treatment, and realizes stable recycling of the catalyst.

The invention discloses a coal washing water efficient solid-water separating agent and method, and belongs to the technical field of coal washing water treatment. The solid-water separation agent comprises a solid-water separation agent A and a solid-water separation agent B. The solid-water separation agent A comprises 20% by mass of poly dimethyl diallyl ammonium chloride with the effective content of 40%; the mass fraction of polyaluminum chloride with the effective content of 10% is 20%; the effective content of the polyferric trichloride is 30%, and the mass fraction is 5%. The solid-water separating agent B comprises polyacrylamide, and the mass fraction of the polyacrylamide is 45%; magnesium chloride with a mass fraction of 25%; magnesium chloride with a mass fraction of 15%; sodium sulfate with a mass fraction of 10%; and isopropanol with a mass fraction of 5%. Rapid separation of coal washing water solid-water can be achieved, and the dosage of the coal washing water solid-water separating agent A is 7-20 g, and the dosage of the coal washing water solid-water separating agent B is 2-8 g. The efficient solid-water separating agent for coal washing water has the advantages of being small in dosage, fast in solid-water separation, short in separation time, high in separation efficiency, clear in upper-layer water after precipitation, good in water recycling effect and the like.

The present invention provides a kind of S-type and R-type flurbiprofen splitting method, comprising the steps: the flurbiprofen to be split is dissolved in an organic solvent, and a test solution is prepared; the test solution is split using supercritical fluid chromatography; wherein, the conditions of supercritical fluid chromatography include: mobile phase A is a supercritical fluid, and mobile phase B is an alcohol including carbon atoms of 1 to 3; the stationary phase of the chromatographic column is a silica gel surface coated with amylose tris (3,5-dimethylphenylcarbamate) stationary phase, a silica gel surface coated with amylose tris [(S)-a-methylbenzylcarbamate] stationary phase, a silica gel surface coated with cellulose tris (4-chloro-3-methylphenylcarbamate) stationary phase or a silica gel surface coated with cellulose tris (4-methylbenzoate) stationary phase. The splitting method has a short separation time, a good separation effect, and is more environmentally friendly.

The application discloses a blood separation device, which is suitable for synchronous centrifugal motion with a centrifugal driving device, and comprises a sample bin, a flow guide pipe, a sensing assembly, a valve assembly, a distribution bin and a controller. The sample bin is communicated with the inlet end of the valve assembly through the flow guide pipe. The valve assembly can be switched among three states of closing the inlet end, communicating the inlet end with the first outlet end and communicating the inlet end with the second outlet end. The distribution bin is provided with independent storage cavities communicated with the two outlet ends respectively. The controller controls the valve state switching according to the detection signal of the sensing assembly. The blood component export and component separation are completed by centrifugal force driving. The device can complete component separation without stopping centrifugation, and the pump body and rotary sealing joint are omitted, so that the structure is simplified. The secondary mixing of blood components caused by stopping is avoided, and the separation efficiency and purity are improved. The whole process is automatically operated, and the manual operation and pollution risk are reduced.

The invention discloses a method for separating fibroblasts from human heart tissue, and belongs to the technical field of cell culture. The method comprises the following steps: standing and digesting heart tissues by using digestive juice containing 0.25 mg / mL collagenase II and collagenase IV; and splitting by using a red blood cell lysis solution, resuspending, centrifuging, and discarding the supernatant to obtain the human heart fibroblasts. According to the invention, heart tissues can be fully dissociated, fibroblasts in the heart tissues can be released, and damage to the heart fibroblasts in the digestion process can be reduced to the greatest extent. According to the method, the problems of low cell activity, low cell yield and the like are solved, and the human heart fibroblasts with full cytoplasm and complete cell nucleuses can be obtained 2-4 days after separation. The method is convenient to operate, short in separation time, high in practicability and high in cell quality, and the human heart fibroblasts obtained through separation have wide application prospects in the aspects of cell culture, screening and testing of new drugs, providing of high-quality cell sources and the like.

The invention discloses a method for extracting plasma exosomes based on an ultracentrifugation method, which belongs to the technical field of biomedicine, and comprises the following main steps: firstly, carrying out differential centrifugation to remove visible components, cell debris and large vesicles in blood, ensuring no platelet residue, reducing the possibility that large particulate matters carry exosomes to settle, and extracting exosomes from the exosomes; the subsequent exosome recovery amount is increased; secondly, low-temperature operation is carried out in the whole process, so that the biological activity and the structure of the exosome can be better guaranteed, the degradation of exosome protein and RNA is effectively avoided, and the sample purity is high. According to the method for extracting the plasma exosomes based on the ultracentrifugation method, impurities such as cell debris and protein particles in the plasma are effectively removed by using a 0.22-micron filter for filtration, the purity of the exosomes is improved, and the method for extracting the plasma exosomes based on the ultracentrifugation method is short in separation time, high in repeatability, low in operation cost, large in sample treatment capacity, not prone to introducing personal errors and capable of being used for primary separation of a large number of samples.

The invention relates to a downhole oil-gas-water three-phase efficient separation device suitable for an oil well with super-high liquid yield, which comprises a combined shell and a multi-stage separation structure, the upper end of the combined shell is connected with an upper connector, the lower end of the combined shell is connected with a lower connector, and the lower end of the lower connector is connected with a bridge type channel; an oil discharge channel, an exhaust channel and a water discharge channel are arranged in the wall of the combined shell; the multi-stage separation structure is formed by connecting all stages of separation structures in series in the combined shell from top to bottom; each stage of separation structure is provided with a grading shell and a grading three-phase separator group, each grading three-phase separator group comprises a plurality of three-phase separators which are connected in parallel, an oil phase deposition plate, a degassing composition plate, a gas-liquid separation cylinder, a combined pore plate and an underflow cover, and the degassing composition plate is formed by arranging an upper part plate and a lower part plate on the outer edge of a central column. According to the invention, the multi-stage serial single-stage parallel cyclone separator is applied to an offshore oil field, and the three-stage separation structure works independently, so that the separation efficiency is greatly improved.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for separating related impurities in a dopurazide tablet and determining the content of each component. According to the method, octadecyl silane bonded silica gel is adopted as a filling agent, a potassium hexafluorophosphate solution is adopted as a mobile phase A, methyl alcohol is adopted as a mobile phase B, and benserazide hydrochloride, a benserazide hydrochloride impurity C and a benserazide hydrochloride impurity E are sequentially separated through gradient elution; and then the sample enters a detector with the detection wavelength of 210 nm to be detected. And calculating the content of each component by adopting an external standard method according to the measured chromatogram. The self-established high performance liquid chromatography is not interfered by other impurities or auxiliary materials, and separation and determination of the benserazide hydrochloride, the benserazide hydrochloride impurity C and the benserazide hydrochloride impurity E can be realized within 35 minutes. The method is high in specificity, high in accuracy and good in precision and durability.

This invention relates to a chip separation device for CNC machine tools and the CNC machine tool itself. The separation device includes a frame, a guide channel mounted on the frame, multiple magnetic suction components, a filter component, and a recovery device. The guide channel is inclined to convey a mixture of chips and coolant collected by the machine tool to the filter component. Multiple magnetic suction components are spaced apart along the extension direction of the guide channel. Each magnetic suction component can move independently into the guide channel to magnetically attract the chips in the mixture and can be removed from the guide channel to release the magnetic attraction. The filter component filters out the coolant from the mixture output from the guide channel and separately conveys the coolant and the filtered chips to the recovery device for recycling. This invention removes chips more thoroughly and efficiently. Furthermore, by initially separating the chips with magnetic suction components and then filtering them with the filter component, the processing load on the filter component is reduced, the equipment size is reduced, and the equipment is more conducive to miniaturization.

The application belongs to the technical field of chiral chromatographic separation, and discloses a chiral chromatographic separation and analysis method for homosalate enantiomers. The chiral chromatographic separation and analysis method is used for the first time for homosalate enantiomers, and the chiral chromatographic separation and analysis method selects a mixed solution composed of n-hexane and MTBE (methyl tert-butyl ether) as a mobile phase, and successfully realizes chiral separation of four enantiomers of homosalate on a polysaccharide derivative chiral stationary phase (bonded type straight-chain amylose-tris (3-chloro-5-methyl phenyl urea)). The chiral chromatographic separation and analysis method has high resolution, is simple and easy to operate and control, has low detection cost, and has good stability.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for separating and detecting an ethyl gatetate starting material SM1c and impurities thereof. The initial material SM1c of ethyl tetracate is 2, 4, 5-trifluoro-3-methoxybenzoyl chloride, and the initial material SM1c of ethyl tetracate is 2, 4, 5-trifluoro-3-methoxybenzoyl chloride. The impurities comprise one or more of an impurity SM1c10, an impurity SM1c3, an impurity SM1c13 and an impurity SM1c4. According to the method, a gas chromatographic method is adopted for analysis, a capillary column with (5%-phenyl)-methylpolysiloxane as a stationary liquid is adopted as a chromatographic column, 2, 4, 5-trifluoro-3-methoxybenzoyl chloride and related substances are separated by utilizing a heating procedure, and the 2, 4, 5-trifluoro-3-methoxybenzoyl chloride and the related substances enter a detector for detection; and calculating the content of each impurity by adopting a main component self-contrast method according to the chromatogram. The method has the characteristics of good separation degree, good durability, high sensitivity and good reproducibility.

The invention belongs to the technical field of separation and purification of organic compounds, and particularly relates to a method for separating and preparing cis-trans isomers in vitamin K1. The separation and preparation method of cis-trans isomers in vitamin K1 provided by the invention comprises the following steps: dissolving a vitamin K1 crude product in a first organic solvent to obtain a vitamin K1 solution; carrying out supercritical fluid chromatography separation on the vitamin K1 solution, and collecting corresponding eluents according to retention time in a spectrogram obtained by supercritical fluid chromatography separation to respectively obtain cis-isomer of vitamin K1 and trans-isomer of vitamin K1; a mobile phase for supercritical fluid chromatography separation comprises a mixture of supercritical carbon dioxide and a second organic solvent, and the second organic solvent is n-hexane or n-heptane; the filler in the chromatographic column for supercritical fluid chromatographic separation is silica gel. VK1 cis-trans isomers are well separated under the combination of a specific stationary phase and a mobile phase, and the purity of the obtained trans isomers is greater than 99%.

The invention discloses a method for efficiently preparing water-soluble regenerated fibroin based on solvent replacement, which comprises the following steps: dissolving degummed natural fibroin with a low-boiling-point and high-volatility organic solvent, adding water to stop reaction after complete dissolution, quickly separating the solvent from a regenerated fibroin aqueous solution through rotary evaporation or reduced pressure distillation, and recovering the solvent, thereby obtaining the water-soluble regenerated fibroin. After filtering, freeze-drying to obtain water-soluble regenerated fibroin which can be stored for a long time; the prepared regenerated fibroin is good in water solubility, the technical problems that in the preparation process of the water-soluble regenerated fibroin, the desalination difficulty is large, and the preparation efficiency is low are solved, 'salinization-free 'is achieved in the whole process, no wastewater is generated, the solvent can be recycled, the technological process is shorter, the production process is green and environmentally friendly, and the production efficiency is greatly improved.

The invention discloses an oligonucleotide drug impurity identification method based on central cutting two-dimensional liquid chromatography-mass spectrometry. Comprising the following steps: acquiring an oligonucleotide sample solution; preparing a mobile phase RA and a mobile phase RB of a one-dimensional liquid chromatographic column and a mobile phase LA and a mobile phase LB of a two-dimensional liquid chromatographic column; separating a target component from a to-be-identified sample solution by utilizing a one-dimensional liquid chromatographic column according to a center cutting method to obtain the target component; further separating the target component through a two-dimensional liquid chromatographic column, and desalting on line; and detecting the molecular weight and the sequence of the target product by using a mass spectrometer to obtain the molecular weight and the sequence of the target product. The method covers a wide range of oligonucleotide sequences, and is suitable for purity detection of oligonucleotides with various sequences and different lengths; the method can be combined with mass spectrometry to quickly analyze the purity, the molecular weight and the sequence of the sample.

The invention discloses a preparation method of a tobacco flavor capable of reducing harmful substance residues, which comprises the following steps: adding an aqueous solution or an ethanol solution with the mass concentration of 10-80% into a tobacco raw material, carrying out hot reflux extraction at 30-100 DEG C, and filtering to obtain a tobacco extracting solution. And carrying out vacuum concentration on the tobacco extracting solution at 30-100 DEG C until the density is 1.0-1.1 g / cm < 3 >, so as to obtain the tobacco concentrated solution. Standing the tobacco concentrated solution, and filtering to obtain an external water phase solution; the preparation method comprises the following steps: mixing a surfactant, a carrier and an oil phase, adding an internal water phase solution, and stirring in an emulsification container to prepare a stable W / O / W type emulsion; uniformly mixing an external water phase solution with the W / O / W emulsion, stirring, standing for 10-60 minutes, layering, separating an emulsion phase from a water phase, collecting the water phase, adding propylene glycol with the mass ratio of 10%-20%, and performing vacuum concentration at the temperature of 30-70 DEG C until the density is 1.1-1.3 g / cm < 3 >, so as to obtain the tobacco flavor capable of reducing harmful substance residues. The tobacco flavor obtained by the method disclosed by the invention can effectively reduce the content of harmful substances such as heavy metals, and is very suitable for industrial production.

The present invention utilizes the GC-MS / MS method to separate and extract samples, and can detect 48 free fatty acids in human serum in 25 minutes; the multi-reaction detection mode library is built to eliminate false positive interference, which is more accurate and highly sensitive.

The utility model provides a separate mobile compression box anti -dripping device relates to the field of compression box, this separate mobile compression box anti -dripping device, including stand, the top of stand is provided with drainage ditch, the top one side of stand is fixedly connected with left support framework, the top other side of stand is fixedly connected with right support framework, the top of left support framework is fixedly connected with left guide rail, the top of right support framework is fixedly connected with right guide rail, the inner side of left guide rail and right guide rail all are connected with gyro wheel axle subassembly of rolling, the side of gyro wheel axle subassembly is provided with axle, the inner side of axle is provided with bearing, the outside of bearing is provided with roller, the side of bearing is provided with baffle, this separate mobile compression box anti -dripping device can completely catch the residual drip percolate, and the percolate flows into drainage ditch through steel wire hose, and the concentrated processing does not need manual collection and cleaning.