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Microwave-assisted aqueous two-phase/reversed micelles extraction method of Sophora flavescens alkaloids

A microwave-assisted, reverse micelle technology is applied to medical preparations, pharmaceutical formulations, plant raw materials and other directions containing active ingredients, which can solve the problems of low purity, low extraction rate, and no extraction is investigated, and achieves good purification effect. The effect of simplified purification steps and no pollution equipment requirements

Inactive Publication Date: 2012-11-14
GUANGDONG PHARMA UNIV
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Problems solved by technology

However, this study only carried out extraction with AOT reverse micelles, and did not investigate and compare the extraction of Sophora flavescens alkaloids with different reverse micellar systems, and the extraction rate obtained is also low, and the purity is not high, which needs to be improved and improved

Method used

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  • Microwave-assisted aqueous two-phase/reversed micelles extraction method of Sophora flavescens alkaloids
  • Microwave-assisted aqueous two-phase/reversed micelles extraction method of Sophora flavescens alkaloids
  • Microwave-assisted aqueous two-phase/reversed micelles extraction method of Sophora flavescens alkaloids

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Embodiment Construction

[0038] The present invention will be further described below in conjunction with specific examples, but it is not limited thereto.

[0039] A method for separating Sophora flavescens alkaloids through microwave-assisted two-phase-reverse micellar extraction, comprising the following steps:

[0040] (1) Preparation of ethanol-ammonium sulfate two-phase extractant:

[0041] Weigh ammonium sulfate, add water and ultrasonically dissolve it, add a certain volume of absolute ethanol, stir well, let stand, and separate phases to obtain ethanol-ammonium sulfate two-phase extractant, wherein the mass fraction of ammonium sulfate is 18%, and the mass fraction of ethanol is The score is 28%.

[0042] (2) Preparation of SDBS-isooctane-n-octanol reverse micellar extractant:

[0043] Add SDBS to the solvent isooctane and co-solvent n-octanol (the volume ratio of isooctane to n-octanol is 4:1), ultrasonically dissolve it, add KCl aqueous solution with a concentration of 0.05 mol / L, and hea...

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Abstract

The invention discloses a microwave-assisted aqueous two-phase / reversed micelles extraction method of Sophora flavescens alkaloids. The method comprises the steps of extracting Radix Sophorae Flavescentis or Radix Sophorae Tonkinensis by use of microwave-assisted ethanol-ammonium sulfate aqueous two-phase system, in which Sophora flavescens alkaloids are partitioned between the two aqueous phases and extracted into the upper phase to complete primary purification; selectively extracting Sophora flavescens alkaloids by use of SDBS (sodium dodecyl benzene sulfonate) / isooctane / n-octanol reversed micelles system, to obtain Sophora flavescens alkaloids crude product with low impurity content; and performing separation and purification to obtain matrine, oxymatrine and sophoridine. The method combines reversed micelles extraction, microwave-assisted extraction and aqueous two-phase extraction technologies, to realize advantage complementation, simple method, high speed and efficiency, no pollution, low requirements for facilities, easy continuous production and scale-up. The extracted Sophora flavescens alkaloids have purity above 90.92% and yield up to 55.38 mg / g. The separated and purified matrine, oxymatrine and sophoridine have high purity above 94% and little organic solvent residue.

Description

technical field [0001] The invention relates to a method for extracting alkaloids, in particular to a method for separating alkaloids from Sophora flavescens by microwave-assisted two-phase-reverse micellar extraction. Background technique [0002] At present, there are few research reports on the use of aqueous two-phase system (ATPS) for the separation and purification of active ingredients of Chinese herbal medicines. Lin Qiang et al. used the new system of ethanol / dipotassium hydrogen phosphate to extract the effective components in licorice, investigated the solvent, the type of salt and other factors, and optimized the extraction conditions. Under the optimal extraction conditions, the concentration of glycyrrhizinate in the two phases The distribution coefficient can reach 12.8, the yield can reach as high as 98.3%, and the method is simple and easy. In addition, ATPS is also used in the extraction and separation of flavonoids. Zhang Chunxiu et al. used the PEG / phos...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/489
Inventor 范华均
Owner GUANGDONG PHARMA UNIV
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