Multifunctional high performance counter current chromatograph

Abstract

The invention relates to a multifunctional highly efficient counter current chromatograph, which consists of a disc type counter current chromatographic separation column, a counterweight body, a planet carrier, a cylinder rotating axis, a planet wheel, a center wheel, a central revolution axis, an electrical motor, a transmission device, a speed control device and an insulated chassis. The disc type counter current chromatographic separation column comprises at least a or a plurality of discs provided with designing slots, clapboards at two sides of each disc, the cylinder rotating axis, an upper flange and a lower flange; the cylinder rotating axis is hollow and passes through the center of the upper flange, the lower flange, the discs and the clapboards; the inner edge and the outer edge of the upper flange, the lower flange, the discs and the clapboards are provided with a plurality of bolt pieces that fix the upper flange, the lower flange, the discs and the clapboards together. The advantages of the invention lie in that: the fixed phase retention value of a two-phase system comprising an organic phase is further promoted and highly efficient and fast separation of micromolecular substances is realized in high flowing speed. Meanwhile, the retention rate of an aqueous two-phase system is remarkably prompted, thus being applicable for the separation of biomacromolecule.

Description

Technical field [0001] The invention relates to the technical field of countercurrent chromatographic separation, and is a multifunctional high-efficiency countercurrent chromatograph. Background technique [0002] Countercurrent chromatography (countercurrent chromatography, CCC) is a continuous liquid-liquid partition chromatographic separation technology, developed into high-speed countercurrent chromatography (High-speed, HSCCC) in the 1980s. In recent years, natural biological active ingredients, especially It shows many unique advantages in the separation and purification of small molecule components. Since it does not require a solid support, the separation of substances is realized based on the difference of their distribution coefficients in two immiscible liquids, thus avoiding the problems of irreversible adsorption, deactivation and denaturation of the separated substances. Moreover, since the material to be separated and the liquid stationary phase can be fully conta...

AI Technical Summary

Problems solved by technology

However, its separation speed and separation efficiency need to be further improved.

[0004] For the high-viscosity, low interfacial tension two-phase solvent system necessary for the separation of biomacromolecules, such as the two-phase aqueous polymer system, due to its high viscosity, small diffusion coefficient, small difference in density between the two phases, and long delamination time, in There is a tendency of emulsification in the column, so that the retention value of the stationary phase of the commonly used polymer system on the HSCCC instrument is lower than 10%, thus limiting the application of high-speed countercurrent chromatography in the separation of biological macromolecules

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