66 results about "Irreversible adsorption" patented technology

The invention discloses an easily implemented lithium pre-insertion novel method for a cathode of a lithium ion super capacitor, wherein, a nonmetal lithium third electrode is led into a novel lithium ion super capacitor system which takes porous charcoal materials, conductive polymers or compounds of the conductive polymers as an anode, lithium insertable metal oxides or charcoal lithium insertion materials as the cathode and organic lithium salt solution as electrolyte, and lithium pre-insertion processing of the cathode with a depth of 5 to 60 percent is performed so as to prevent reduction of the ion concentration of the electrolyte during the charging and discharging process and non-reversible absorption of anions on the anode and improve the charging and discharging characteristics of the capacitor. The essential component of the nonmetal lithium third electrode is lithium-rich compounds with non-reversible lithium-free property; during the preparation process of the porous charcoal material anode or the conductive polymer anode, the lithium-rich compounds of the third electrode are mixed by mass percent of 3 to 50 and can form the lithium ion super capacitor together with the lithium insertable cathode after being prepared into the electrode; during the activation process of the capacitor, lithium pre-insertion of the cathode is realized.

The invention provides a method for separating cyanidin in nitraria sibirica pall through high-speed counter-current chromatography. With utilization of the high-speed counter-current chromatography and adopting of a reasonable solvent system, the high-purity cyanidin is obtained from nitraria sibirica pall; the method requires no use of a solid-phase carrier, has no irreversible adsorption, and separates and prepares cyanidin anthocyanin with no loss of samples, no pollution, high efficiency, speediness and low cost. The method has the advantages of simple process, convenient operation, safety, and good reproducibility, and can be used for industrialized production.

The invention discloses a method for preparing salvianolic acid B and rosmarinic acid by adopting a high-speed counter-current chromatography separation and purification process. The method comprises the following steps: grinding salvia miltiorrhiza, performing ethanol extraction, enriching through macroporous adsorption resin to obtain a crude extract, and performing high-speed counter-current chromatography separation on the crude extract by a high-speed counter-current solvent system consisting of solvents A, B, C and D to prepare salvianolic acid B and rosmarinic acid respectively, wherein the volume ratio of the solvent A to the solvent B to the solvent C to the solvent D is (1-5) to (5-10) to (1-5) to (1-10); the solvent A is methanol, ethanol, n-hexane or iso-hexane; the solvent B is ethyl acetate, propyl acetate or n-butyl acetate; the solvent C is methanol, ethanol or acetone; the solvent D is water; an upper phase is a stationary phase; a lower phase is a mobile phase. According to the method, the salvianolic acid B and the rosmarinic acid are prepared by adopting the high-speed counter-current chromatography process, inreversible adsorption is not existent, and the sample loss is avoided; the method has the characteristics of having a good separation effect and being low in solvent consumption, pollution-free, efficient and quick.

The invention relates to a preparation method of high-dietary-fiber oleogel. The preparation method comprises the steps of preparing cellulose water dispersion, mixing the cellulose water dispersion and hydrophilic colloid, dispersing oil phase in the mixed system to prepare oil-in-water emulsion, and treating the oil-in-water emulsion to obtain the oleogel, or adding food additives to promote the emulsion to form gel to obtain the oleogel, wherein the treatment method comprises a drying method and a freezing method. Cellulose particles service as emulsifiers, the hydrophilic colloid serves as a stabilizer and a thickening agent, the cellulose particles are irreversibly adsorbed on the oil water interface, the hydrophilic colloid intersperse the cellulose network structure to jointly form a three-dimensional network structure, liquid fat can be constrained efficiently, the prepared emulsion gel has high stability, and long-time storage and transportation are facilitated; the method is simple, convenient, high in dietary fiber content of the prepared high-dietary-fiber oleogel, economic and environmentally friendly; the oleogel is physical gel, does not change the chemical property of the oil phase, avoids generation of trans-fatty acid, and has good application prospect in the fields of food, medicines and cosmetics.

The invention relates to a method for extracting and preparing high-purity ginsenoside Re from herminium by high speed counter current chromatography, which is characterized by comprising the following steps: soaking fruit residues of the herminium in 70 percent ethanol solution through overnight; performing ultrasonic extraction for 30 minutes for three times repeatedly and concentrating extract liquid after filtration to obtain herminium coarse extract; and selecting ethyl acetate 0.5-2, butanol 4-7 and water 6-9 according to a volume ratio to form a solvent system and separating at a room temperature of from 16 to 28 by using a high speed counter current chromatographic instrument. The method for preparing and separating ginsenoside Re in large scale avoids the use of solid filler as well as irreversible adsorption, sample loss and pollution, and is high in efficiency, quick and low in cost. The obtained ginsenoside Re has a purity of more than 98 percent, and the method is suitable for separating and preparing the insenoside Re from herminium coarse extracts prepared under various process conditions.

The invention provides a method for separating petunidin in lycium ruthenicum murray through high-speed counter-current chromatography. With utilization of the high-speed counter-current chromatography and adopting of a reasonable solvent system, the high-purity petunidin is obtained from lycium ruthenicum murray; the method does requires no use of a solid-phase carrier, has no irreversible adsorption, and separates and prepares petunidin anthocyanin with no loss of samples, no pollution, high efficiency, speediness and low cost; the purity of the obtained petunidin can reach more than 97%. The method has the advantages of simple process, convenient operation, safety and good reproducibility, and can be used for industrialized production.

This invention relates to a method of preparing high-purity Schisandrin B. The invention adopts analysis mode high speed counter-current chromatography to proceed small scale preparation, adopt semipreparative type high speed counter-current chromatography to proceed large-scale preparation. The feature is that in solvent system the volume ratio of normal hexane, methanol, water and acetonitrile is 5 - 11 : 3 - 6 : 2 - 5 : 1, and the best volume ratio is 10:5:4:3. The invention does not need solid padding, has no irreversible adsorption and no pollution.The purity of gained Schisandrin B can reach 98%upwards.

This invention relates to a method of preparing high-purity gentiopicrin. The invention adopts small scale preparation by analysis mode high speed counter-current chromatography, and large-scale preparation by semipreparing type high speed counter-current chromatography; volume ratio of chloroform, methanol, normal butyl alcohol and water is 4-8:3-6:1-4:3-6; one time separation to gain gentiopicrin. No need solid filling and without irreversible adsorption. The acquired gentiopicrin purity reach 92%upwards, lend itself to prepare gentiopicrin from different kinds of rough gentian crude extract.

The present invention discloses a capillary electrochromatographic open tubular column with a mixed effect of hydrophilia and ion exchange and a preparation method of the same. The convergence layer of the open tubular column comprises methyl methacrylate ionic monomers, 2-methyl-3-butene cyanide polar monomers, initiators and cross-linking agents, wherein the ionic monomers account for 3.5-7.0 percent by weight, the polar monomers account for 8.0-27.0 percent by weight, the cross-linking agents account for 3.5-7.0 percent by weight, the initiators account for 0.3-1.0 percent by weight, the inert solvents account for 60.0-90.0 percent by weight. The bonded cyano structure in the open tubular column has a function of hydrophilic chromatography, carboxyl groups can be dissociated to generate a positive electroosmotic flow, ion exchange can occur between the carboxyl groups and basic amino positive charges, and the problems that polar alkaline materials are absorbed on a capillary column irreversibly and cannot be separated easily are solves under the combined effect of the carboxyl groups and the basic amino positive charges. The functional coating of the polar capillary open tubular column is modified via chemical bonding, and the open tubular column has a mixed mode of hydrophilic chromatography and ion exchange, has a good effect on separation of positive charge type alkaline drugs, has good reproducibility, and is suitable for capillary electrochromatographic separation and analysis of polar drugs.

The invention introduces a method for pre-insertion of lithium of a lithium ion supercapacitor negative electrode. A positive electrode containing 2-15% of lithium-rich compound by mass content, a negative electrode capable of pre-insertion of lithium and a separator are assembled to be a lithium ion supercapacitor, the lithium ion supercapacitor is put into a container, electrolyte is injected into the container to charge the lithium ion supercapacitor to achieve pre-insertion of the lithium with 30-90% by the mass of the theoretical pre-insertion of lithium for the negative electrode of thepre-insertion of the lithium. The negative electrode is subjected to pre-insertion of the lithium to avoid the reduction of the concentration of the ions of the body in the electrolyte and the irreversible absorption of the negative ions at the positive electrode so as to achieve the purpose of improving the charge-discharge performances of the lithium ion supercapacitor.

The invention provides a method for separating and purifying stilbene glucoside monomers, which adopts high-speed counter-current chromatography and uses a ternary solvent system composed of ethyl acetate, ethanol and water as a separation solvent system to separate and extract the stilbene glucoside monomers from a crude stilbene glucoside product. The separating and purifying method of the invention adopts a semi-preparative high-speed counter-current chromatograph to separate and purify the crude stilbene glucoside product, allows the crude stilbene glucoside product to go through the ternary solvent system, and utilizes the characteristics of high recovery rate and no irreversible adsorption of the high-speed counter-current chromatography to massively prepare the stilbene glucoside monomers, and the preparation amount can reach a gram magnitude; and the purity of the prepared stilbene glucoside monomers, which is high, reaches above 99%, and a standard sample of the stilbene glucoside monomers can be prepared with the high purity stilbene glucoside monomers as a raw material. The method of the invention is suitable for different crude stilbene glucoside products, and has the advantages of advanced technology, high separation efficiency, good recovery rate, high product purity, large preparation amount and the like.

The invention provides a microbial preparation method of dibutyl phthalate. The microbial preparation method of the dibutyl phthalate specifically comprises the following steps: performing strain liquid fermentation by using fungus Verticillium lecani or Beauveria tenella; extracting fermented liquid; and performing separation and purification by high-speed countercurrent chromatography to obtainthe dibutyl phthalate. The dibutyl phthalate is prepared by a microbial fermentation method; compared with a liquid-solid chromatography, the microbial preparation method of the dibutyl phthalate hasthe advantages that: sample preprocessing work is simplified, a solid supporting body is not used, irreversible adsorption, denaturation, inactivation, tailing and other phenomena of a sample component are avoided, the separation capability is strong, the separation efficiency is high and the separation time is short; and compared with a chemical synthesis method, the microbial preparation methodof the dibutyl phthalate is simple, low in cost and free of pollution in a production process.

The invention relates to a method for preparing eriocalyxin B with high purity from crude extract of lamiaceae plectranthus genus plant flower thinning isodon eriocalyx by applying high speed counter-current chromatography, which is characterized in that two solvent systems which are not mixed and dissolved with each other are applied for separating and purifying natural products in a support tube in a way of high-speed planetary motion, so that irreversible adsorption is not available, the method has the advantages of no loss of sample, no pollution, high efficiency, high speed and large amount of separation, the obtained eriocalyxin B has the purity of more than 98%, and method is applicable to separating and preparing eriocalyxin B monomer from flower thinning isodon eriocalyx crude product prepared under various technical conditions and is suitable for separating and preparing the eriocalyxin B monomer by using counter current chromatographs with various models. The preparation method has simple operation, less separating steps, high efficiency, low loss of solvent and high product purity, and is suitable for industrialized production.

The invention relates to a separation method of natural medicines, and specifically relates to a method for separation and preparation of spermidines from safflower by employing high-speed counter-current chromatography. The method comprises the following steps: (1) mixing chloroform, methanol and aqueous solution to form a mixed solvent, layering; (2) dissolving safflower spermidine crude extract in the lower layer of the mixed solvent obtained in the step (1); (3) layering the mixed solvent in the step (1) for obtain a solid phase and a mobile phase, injecting the solution in the step (2) into chromatographic column via an admission valve, continuously pumping the mobile phase after injection is finished; and (4) collecting isolates and concentrating to obtain first spermidine, a second spermidine, third spermidine and fourth spermidine. By employing the high-speed counter-current chromatography and the reasonable solvent system, high-purity spermidine compounds are extracted from safflower; on solid-phase carrier is needed, and no irreversible adsorption exists, spermidines are prepared efficiently rapidly with low cost and without sample loss and without pollution. The method is simple in technology, convenient to operate, safe, good in repeatability, and applicable to industrialized production.

The invention discloses a method for preparing harpagoside and sibirioside A by using high-speed counter-current chromatography through separation and purification. The method comprises the steps of crushing a radix scrophulariae medicinal material, extracting by ethanol, and enriching by using macro-porous adsorption resin to obtain a crude extract; performing high-speed counter-current chromatography separation on the crude extract; forming a high-speed counter-current solvent system by using a solvent A, a solvent B and a solvent C of which the volume ratio is (1-5): (5-10): (1-10), wherein the solvent A is methanol, ethanol, n-butyl alcohol or isobutanol; the solvent B is ethyl acetate, propyl acetate or n-butyl acetate; the solvent C is water; an upper phase is a stationary phase, and a lower phase is a mobile phase, so that harpagoside and sibirioside A are prepared respectively. The method disclosed by the invention is used for preparing harpagoside and sibirioside A by using the high-speed counter-current chromatography, does not have irreversible adsorption, avoids the loss of samples, and has the characteristics of good separation effect, small solvent dose, no pollution, high efficiency and high speed.

The invention provides a method for separating and purifying puerarin monomers, which adopts high-speed counter-current chromatography and uses a ternary solvent system composed of ethyl acetate, n-butanol and water as a separation solvent system to separate and extract the puerarin monomers from a crude puerarin product. The separating and purifying method of the invention adopts a semi-preparative high-speed counter-current chromatograph to separate and purify the crude puerarin product, allows the crude puerarin product to go through the ternary solvent system, and utilizes the characteristics of high recovery rate and no irreversible adsorption of the high-speed counter-current chromatography to massively prepare the puerarin monomers, and the preparation amount can reach a gram magnitude; and the purity of the prepared puerarin monomers, which is high, reaches above 99%, and a standard sample of the puerarin monomers can be prepared with the high purity puerarin monomers as a raw material. The method of the invention is suitable for different crude puerarin products, and has the advantages of advanced technology, high separation efficiency, good recovery rate, high product purity, large preparation amount and the like.

The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a method for separating and purifying amentoflavone, in particular, a method for enriching and purifying the amentoflavone through a high speed countercurrent chromatography method. The method is characterized in that: two mutually-immiscible solvent systems are utilized to perform the separation and purification in a mode of high-speed planetary motion in a support pipe; the method is applicable for the countercurrent chromatographies with various types to separate the amentoflavone, and has advantages of no irreversible adsorption, no loss of sample, no pollution, high efficiency and speed, high recovery rate, mild separating environment, high product purity and large quantity separation, and is applicable for industrial production.

The invention discloses a carbon composite material, mixed paste, a battery and a preparation method, and belongs to the technical field of lead-carbon battery negative electrode materials. The carboncomposite material takes high-conductivity graphite as an inner core, the surface is coated with activated carbon with a high specific surface, and a core-shell structure of which the graphite surface is coated with activated carbon is formed. Meanwhile, the problems of poor conductivity of activated carbon, irreversible adsorption of lignin, serious hydrogen evolution and the like are solved, the quick charging performance of the lead-carbon battery can be remarkably improved, the water loss of the battery is reduced, and the cycle life of the lead-carbon battery is prolonged.

The invention belongs to the technical field of materials, and discloses a stable Pickering emulsion based on black tea nano-aggregates and a preparation method and application of the stable Pickeringemulsion. The preparation method comprises the following steps that (1) a black tea water extract is extracted, and the black tea nano-aggregates are separated by ultrafiltration centrifugation; and(2) the black tea nano-aggregates are used as an emulsion stabilizer to cause irreversible adsorption at an oil-water interface to obtain the stable Pickering emulsion. According to the stable Pickering emulsion based on the black tea nano-aggregates and the preparation method and application of the stable Pickering emulsion, the nano-aggregates of different types of black tea are used as the Pickering emulsion stabilizer, the sources are natural, the nano-aggregates are green and healthy, and the Pickering emulsion can be effectively stabilized without modification; and in addition, substances such as theabrownins, tea polyphenols and tea polysaccharides in the black tea nano-aggregates have anti-oxidation, lipid-lowering and weight-reducing effects, the efficacy of bioactive components of the black tea nano-aggregates can be imparted to the Pickering emulsion, the application fields of the Pickering emulsion are broadened, and the application value of the Pickering emulsion is increased.

The invention discloses a method for simultaneously preparing high-purity podophyllotoxin and deoxidized podophyllotoxin from sabina vulgaris. The method takes a high-speed counter-current chromatography device as separating and purifying equipment and comprises the following steps of: preparing a solvent system which forms an immobile phase and a mobile phase, and pressing and suffusing the prepared immobile phase into a working drum of a main machine in an air pressure mode to rotate the main machine, wherein an upper phase is the immobile phase and a lower phase is the mobile phase; then pumping dissolved ethanol extraction extract of sabina vulgaris and the mobile phase at a preset flow rate, and respectively collecting and podophyllotoxin and deoxidized podophyllotoxin components (obtained through separation) from the mobile phase according to a detection spectrogram. The liquid-liquid partition chromatography separation and preparation method disclosed by the invention completely overcomes the defects of irreversible adsorption, contamination, denaturation and the like of a sample on a solid filler and is simple and convenient to operate, low in organic solvent consumption and high in separation efficiency; and the purity of the obtained product is higher than 98%.

The invention relates to a preparation method of a dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column. According to the invention, under the effect of an external magnetic field, magnetic nano-sized particles are orientedly distributed on the inner wall of the capillary tube, such that a nano-sized particle solid-phase bed layer with a certain thickness is formed. An advantage of core-shell-structured silica coated magnetic nano-sized particles of extremely high specific surface areas and an advantage of the open-tubular capillary electric chromatography column of fast separation are combined, such that the dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column is constructed. With the electric chromatography column provided by the invention, column capacity and column efficiency are greatly improved. With a simple action of turning on/off the magnetic field, polluted or irreversibly adsorbed solid phases can be removed, such that the reclaiming of the dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column is realized, and the service life of the capillary column is prolonged. The character is especially suitable to be used in separations of complex samples such as irreversibly adsorbed protein with solid phase of no separation capacity.

The invention relates to a gas detection probe using a multi-phase catalyst alloy and a far infrared ceramic filter and capable of performing back flushing washing and rapid calibration, and belongs to the field of safety protection, wherein the gas detection probe is suitable for catalytic combustion, semiconductor type gas sensors. According to the present invention, the filtering capacity is unaffected by humidity, the absorption rate on the detected gas is low, and the service life is long; the n-shaped porous polytetrafluoroethylene core shell, the internal porous multi-phase catalyst alloy, the internal negative ions and the internal far infrared ceramic catalyst assembly are the core components of the gas detection probe; the n-shaped porous polytetrafluoroethylene core shell has water repellency and provides non-invasion on most solvents, and the porous ceramic catalyst assembly provides irreversible adsorption and high reaction activity on sulfur-silicon-based gas while does not provide reaction activity on hydrogen, alkane gases and carbon monoxide gas, and does not adsorb, such that the sulfur-silicon-based interference gas can be efficiently filtered out; and with the standard gas input and the gas washing back flushing pipe component, the great convenience is provided for the gas sensor calibration and the back flushing washing, and the benefits are provided for the prolonging of the service life.

The invention discloses a novel negative electrode lithium pre-embedding method. In the process of preparing a porous carbon or conductive polymer and a composite positive electrode thereof, a lithium-carbon binary compound is added, wherein the lithium-carbon binary compound accounts for 10-30% of the mass of the positive electrode, after the electrode is prepared, the electrode can be assembledwith lithium-embeddable metallic oxide or carbon lithium-embedded negative electrode to form a lithium ion supercapacitor; the lithium carbide in the positive electrode is decomposed through a first-circle charging process to enable the metal lithium to enter the negative electrode, so that 50-85% of lithium pre-embedding treatment is performed on the negative electrode; and after a high-content lithium-source lithium-carbon compound is added into the positive electrode and is subjected to first-circle formation, the performance of the capacity of the positive electrode is influenced. The negative electrode is pre-embedded with lithium, so that the reduction of the concentration of body ions in the electrolyte and the irreversible adsorption of negative ions on the positive electrode in the charging and discharging process can be prevented to a certain extent, and the purpose of improving the charging and discharging performance of the lithium ion supercapacitor is achieved.

The invention relates to a method for separating and purifying paclitaxel and cephalomannine. According to the method disclosed by the invention, a preparative high performance liquid chromatography is adopted, and the chromatographic conditions are that a polystyrene-divinyl benzene copolymer chromatographic column refers to MKF-EP-RP with the diameter of 5-12 microns and the size of (150-600)*(7.0-20.0)mm (I.D.), a mobile phase comprises acetonitrile and water according to a volume ratio of (45-60) to (40-55), the flow velocity is equal to 1.0-3mL/min, the detection wavelength is equal to 229nm and the sample size is equal to 20-100 microL. According to the method disclosed by the invention, the defects such as irreversible adsorption of media such as silica gel and complicated process flow are overcome, and paclitaxel products with the purity of over 98 percent can be obtained by further separation.

This invention relates to a preparation method of high-purity deoxyschizandrin. The process as follows: the solvent system includes normal hexane, methanol and water; the volume ratio of normal hexane, methanol and water is 2 - 6: 3 - 7: 4 - 8; firstly dissolve deoxyschizandrin crude extract perpared by traditional craft in 2ml - 10ml bottom phase; adopt analysis mode high speed counter-current chromatography to proceed small scale preparation, adopt semipreparative type high speed counter-current chromatography to proceed large-scale preparation. The invention does not need solid padding, has no irreversible adsorption and no pollution. The purity of gained deoxyschizandrin can reach 94%upwards.