Method for ionic liquid aqueous two-phase extraction of flavones and saponins in songaria cynomorium herb

An ionic liquid, two-phase technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, antidote, etc., can solve the problem of inability to extract Cynomorium flavonoids and saponins at the same time, low selectivity and specificity, and flavonoids. To solve the problem of low volume, etc., to achieve the effect of saving resources, high extraction rate, and improving extraction rate

Active Publication Date: 2014-04-02
甘肃凯源生物技术开发中心有限责任公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the separation and extraction of flavonoids in Cynomorium cynomorium by solvent extraction is not only complicated and time-consuming, but also the amount of flavonoids obtained by extraction is not high.
The aqueous two-phase extraction technology often used in the field of separation and purification of biological substances has mild conditions, is easy to scale up, and can be operated continuously. However, the two-phase extraction system used is composed of organic solvents and salts that are miscible with water. , this two-phase extraction system, like traditional organic solvent extraction, has the disadvantages of volatile solvents and large environmental pollution. At the same time, its selectivity and specificity are not high, and flavonoids and saponins in Cynomorium cannot be extracted at the same time , this single extraction causes a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The method for extracting flavonoids and saponins in Cynomorium cynomorium with ionic liquid two-phase aqueous phase of the present embodiment comprises the following steps:

[0031] (1) Cynomorium pretreatment: crush Cynomorium into powder to obtain Cynomorium powder;

[0032] (2) Ultrasonic rough extraction: Take 20 g of Cynomorium powder, add ethanol solution with a volume fraction of 50%, ultrasonically treat for 5 minutes at a power of 400W, and then filter to obtain Cynomorium crude extract; the ethanol solution The volume is 10 times the volume of Cynomorium powder;

[0033] (3) Two-phase extraction: take the Cynomorium Cynomorium crude extract, concentrate it to a mass fraction of 10%, and then add [Bmim]Cl with a mass fraction of 23% and K with a mass fraction of 24%. 2 HPO 4 , forming [Bmim]Cl / K 2 HPO 4 Aqueous two-phase system, and adding sodium chloride with a mass fraction of 1%, adjusting the pH to 5.0, mixing evenly at room temperature, standing and s...

Embodiment 2

[0038] The method for extracting flavonoids and saponins in Cynomorium cynomorium with ionic liquid two-phase aqueous phase of the present embodiment comprises the following steps:

[0039] (1) Cynomorium pretreatment: crush Cynomorium into powder to obtain Cynomorium powder;

[0040] (2) Ultrasonic rough extraction: Take 20 g of Cynomorium powder, add ethanol solution with a volume fraction of 50%, ultrasonically treat for 5 minutes at a power of 400W, and then filter to obtain Cynomorium crude extract; the ethanol solution The volume is 12 times the volume of Cynomorium powder;

[0041] (3) Aqueous two-phase extraction: take the Cynomorium Cynomorium crude extract, concentrate it to a mass fraction of 6%, and then add [Bmim]Cl with a mass fraction of 19% and Na with a mass fraction of 29% 2 HPO 4 , forming [Bmim]Cl / Na 2 HPO 4 A two-phase system, adding sodium chloride with a mass fraction of 2%, adjusting the pH to 3.0, mixing evenly at room temperature, standing and str...

Embodiment 3

[0046] The method for extracting flavonoids and saponins in Cynomorium cynomorium with ionic liquid two-phase aqueous phase of the present embodiment comprises the following steps:

[0047] (1) Cynomorium pretreatment: crush Cynomorium into powder to obtain Cynomorium powder;

[0048] (2) Ultrasonic rough extraction: Take 20 g of Cynomorium powder, add ethanol solution with a volume fraction of 50%, ultrasonically treat for 5 minutes at a power of 400W, and then filter to obtain Cynomorium crude extract; the ethanol solution The volume is 8 times the volume of Cynomorium powder;

[0049] (3) Two-phase extraction: take the Cynomorium crude extract, concentrate it to a mass fraction of 14%, and then add [Omim]Cl with a mass fraction of 27% and Na with a mass fraction of 19%. 2 CO 3 , forming [Omim]Cl / Na 2 CO 3 A two-phase system, adding sodium chloride with a mass fraction of 1%, adjusting the pH to 7.0, mixing evenly at room temperature, standing and stratifying for 10 minu...

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Abstract

The invention discloses a method for ionic liquid aqueous two-phase extraction of flavones and saponins in songaria cynomorium herb. The method comprises the following steps: (1) pretreatment of the songaria cynomorium herb; (2) ultrasonic crude extraction; (3) aqueous two-phase extraction, namely utilizing an ionic liquid / salt aqueous two-phase system to perform extraction; (4) back extraction; (5) vacuum distillation and vacuum drying; (6) recovery of ionic liquid. The method for ionic liquid aqueous two-phase extraction of the flavones and the saponins in the songaria cynomorium herb, disclosed by the invention, has the advantages of simple method, easiness in operation, fast phase splitting, high extraction rate, no need of volatile organic solvents, low environmental pollution and effects of recycling an extraction solution of an extraction system, saving resources, realizing relatively high selectivity and specificity, simultaneously extracting the flavones and the saponins in the songaria cynomorium herb, effectively avoiding the waste of the resources caused by single component extraction, effectively improving the extraction rate of the flavones and the saponins in the songaria cynomorium herb and the comprehensive utilization rate of the songaria cynomorium herb and reducing the deep processing cost of the songaria cynomorium herb.

Description

technical field [0001] The invention belongs to the field of separation and purification of effective components of natural products, and in particular relates to a method for extracting flavonoids and saponins in Cynomorium cynomorium with an ionic liquid two-water phase. technical background [0002] Cynomorium is the dry fleshy stem of Cynomorium cynomorium, a parasitic plant of Cynomorium genus Cynomorium. It often parasitizes Nitraria terrestris. It mostly grows in arid and alkaline sandy land. It is mainly produced in Inner Mongolia, Ningxia, Xinjiang, Gansu, and Qinghai. Wait for the Northwest Territories. Cynomorium is sweet in taste and warm in nature. It has the effects of tonifying kidney, helping yang, benefiting essence, moistening intestines and nourishing tendons. Traditional Chinese medicine is used to treat impotence, nocturnal emission, infertility, soreness of waist and knees, dryness of the intestine and constipation. Cynomorium contains organic acids, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P37/04A61P39/06A61P35/00A61P31/04A23L1/29A23L33/00
Inventor 罗光宏张喜峰祖廷勋王丹霞杨生辉
Owner 甘肃凯源生物技术开发中心有限责任公司
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