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Method of extracting phycocyanin from ionic liquid aqueous two-phase system

A two-phase system, ionic liquid technology, applied in the field of extracting phycocyanin from spirulina, can solve the problems of poor enzyme selectivity, long separation cycle, low separation efficiency, etc., to reduce operating costs and energy consumption, save operation time, the effect of simplifying the operation process

Inactive Publication Date: 2014-06-25
QINGDAO UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention aims to solve the disadvantages of poor selectivity of the extractant to enzymes, low separation efficiency and long separation period in the rough extraction process of conventional spirulina protein, and proposes a two-phase aqueous system composed of ionic liquid, dipotassium hydrogen phosphate and water Efficient and selective extraction of phycocyanin from spirulina

Method used

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  • Method of extracting phycocyanin from ionic liquid aqueous two-phase system
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  • Method of extracting phycocyanin from ionic liquid aqueous two-phase system

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Embodiment 1

[0021] 1) The crushing treatment of Spirulina cells. Take 100g of dry Spirulina platensis algae powder and add 1.5L of 0.05mol / L of K 2 HPO 4 In the aqueous solution, soak for 4 hours at a temperature of 20°C. After soaking, put it into an ultrasonic breaker with an ultrasonic frequency of 40kHz, a power of 1000W, and a temperature of 30°C. Turn on the ultrasonic to break the wall of the Spirulina cells for 15 minutes.

[0022] 2) Ionic liquid two-phase extraction. 225g ionic liquid, 75g K 2 HPO 4 Add to the crude extract of phycocyanin, stir well to make the solid K 2 HPO 4 And the ionic liquid can be quickly dissolved in the crude extract, and effectively form a two-phase system, and the extraction process ends after 90 minutes of extraction. The extraction process is over. The solid is removed by filtration and separation, the liquid part is left to stand for stratification, and the upper layer is an ionic liquid layer containing phycocyanin.

[0023] 3) Separation...

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Abstract

The invention discloses a method of extracting phycocyanin from an ionic liquid aqueous two-phase system and particularly relates to a method of extracting phycocyanin from spiral seaweeds. The method comprises the following steps of: mechanically crushing spiral seaweed materials, adding into proper amount of dipotassium phosphate aqueous liquor and soaking, placing into an ultrasonic crusher to carry out wall-breaking treatment on spiral seaweed cells; extracting crude extracting liquor of spiral seaweed phycocyanin by utilizing the ionic liquid aqueous two-phase system formed by ionic liquid, dipotassium phosphate and water; and adopting column chromatography for separating and purifying the ionic liquid containing the phycocyanin to obtain the phycocyanin fine powder.

Description

technical field [0001] The invention relates to a method for extracting phycocyanin by using an ionic liquid two-phase system, in particular to a method for extracting phycocyanin from spirulina. Background technique [0002] Spirulina is a filamentous microalga rich in protein, vitamins, essential amino acids, minerals and essential fatty acids. The plant protein content of Spirulina is as high as 50-75%, which is equivalent to 1.7 times that of soybeans, 3.1 times that of chicken, and eggs. 4.6 times that of whole milk powder, and 2.9 times that of whole milk powder, and the protein contained is high-quality protein, which is easy to absorb. source. The fat content of spirulina is only 4-6%, the saturated fatty acid contained is very little, and the content of unsaturated fatty acid is as high as 1.7%. Spirulina contains 12 kinds of minerals, 8 kinds of vitamins and a variety of biologically active substances. The cellulose content of spirulina is 2-4%, its cell wall is...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/14
CPCC07K14/795
Inventor 李美凤
Owner QINGDAO UNIV
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