A preparation method of olive leaf extract, comprising the following steps: (1) superfine grinding of olive leaf raw material, continuous countercurrent ultrasonic extraction; (2) extract liquid treatment to obtain centrifuge supernatant and centrifuge residue; (3) centrifuge residue Dissolving, resin adsorption, elution, concentration, and drying to obtain luteolin product; (4) Take step (2) centrifugal supernatant, add ethanol solution, let stand, filter, recover ethanol, add water to dilute, and pass the solution through macroporous resin Adsorption, elution, collection of ethanol eluate, concentration, and drying to obtain hydroxytyrosol products, oleuropein products, and verbascoside products. The invention has high utilization rate of raw materials and is environmentally friendly; the process is simple and the industrialized production is easy to realize. The product prepared by the method has light color, light smell and controllable water solubility, and has a good market prospect in the fields of nutrition, health care and daily chemical industry.