30 results about "Olea europaea leaf" patented technology

The invention discloses a method and an apparatus used for preparing an olive leaf extract. The method comprises the steps that: olive leaves are subjected to ultrasonic vacuum boiling extraction by using a mixed solvent; vacuum pump-filtration is carried out, such that a clarified liquid is obtained; fat-soluble impurities are removed through petroleum ether extraction, and a lower-layer clear solution is obtained; the clear solution is subjected to rotary evaporation until no alcohol smell is left in the solvent; the solution is diluted; the diluant is subjected to macroporous resin purification; the obtained solution is subjected to standing for a period of time, such that oleuropein and flavonoid substances are sufficiently absorbed; the oleuropein is eluted by using an ethanol-water mixed solvent with a proper concentration, and a target liquid is collected; the collected target liquid is subjected to dextrane gel purification; standing is carried out, and an ethanol-water solution is used for eluting; a eluant containing effective components is collected; the collected eluant is subjected to rotary evaporation until no alcohol smell is left; and the obtained material is dried, such that the extract is obtained. The oleuropein content of the extract reaches 85-92%, and the total flavonoid content of the extract reaches 25-35%. The method has the advantages of simple operation, short extraction time, high efficiency, high product recovery rate, good quality, and stable quality.

The invention discloses a method for extracting Olea europaea leaf polyphenol by an aqueous two phase system. The method comprises the following steps of adding dried and crushed Olea europaea leaves into the aqueous two phase system containing hydrophilic low molecules organic solvent and inorganic salt solution, mixing, performing ultrasonic extraction, and extracting the Olea europaea leaf polyphenol to an upper phase; performing enrichment on the upper phase with macroporous resin, eluting the upper phase with the organic solvent, and concentrating and drying eluant to obtain the Olea europaea leaf polyphenol; the recovery rate of the Olea europaea leaf polyphenol is more than 90 percent, and the purity of the Olea europaea leaf polyphenol is more than 80 percent. The aqueous two phase extraction technique, which is a new isolation technique, is applied to isolation and extraction of the Olea europaea leaf polyphenol by combing with the traditional ultrasonic-assisted organic solvent extraction technique, therefore the method has the advantages that the product yield is high, the product purity is high, extraction is rapid, the condition is mild, equipment is simple, the Olea europaea leaf polyphenol is easy to amplify, and the like.

A preparation method of olive leaf extract, comprising the following steps: (1) superfine grinding of olive leaf raw material, continuous countercurrent ultrasonic extraction; (2) extract liquid treatment to obtain centrifuge supernatant and centrifuge residue; (3) centrifuge residue Dissolving, resin adsorption, elution, concentration, and drying to obtain luteolin product; (4) Take step (2) centrifugal supernatant, add ethanol solution, let stand, filter, recover ethanol, add water to dilute, and pass the solution through macroporous resin Adsorption, elution, collection of ethanol eluate, concentration, and drying to obtain hydroxytyrosol products, oleuropein products, and verbascoside products. The invention has high utilization rate of raw materials and is environmentally friendly; the process is simple and the industrialized production is easy to realize. The product prepared by the method has light color, light smell and controllable water solubility, and has a good market prospect in the fields of nutrition, health care and daily chemical industry.