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Preparation method of olive leaf total flavonoids

A technology of olive leaves and total flavonoids is applied in the directions of medical preparations, pharmaceutical formulations, plant raw materials and the like containing active ingredients, which can solve the problems of time-consuming, shortened time, environmental pollution and the like, and achieves avoiding damage, low production cost, and extraction high rate effect

Inactive Publication Date: 2020-05-26
南京艾蒙利特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the solvent extraction methods, the ethanol extraction method is the most efficient, but the product components are complex and time-consuming; the microwave method saves energy and greatly shortens the time, but the leaked microwave is more likely to cause environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Dry the olive leaves in the shade, crush them, add 50% ethanol that is 1 times the amount of raw materials, place them in a homogenizer, and mix and homogenize them for 15 minutes under the conditions of 200W and 10000r / min; In the device, add 50% ethanol solution 3 times the raw material, conduct ultrasonic extraction at 50°C for 90 minutes, concentrate under reduced pressure, recover ethanol, and obtain a concentrated solution; add the obtained concentrated solution to 80-mesh polyamide with the same quality as the raw material In the resin column, after elution of 70% of 4 times the volume of the resin, the eluate was collected, concentrated and dried to obtain the total flavonoids of olive leaves, the extraction rate was 92.9%, and the h content of the total flavonoids was 87.53% by UV detection.

Embodiment 2

[0019] Dry the olive leaves in the shade, crush them, add 60% ethanol twice as much as the raw materials, place them in a homogenizer, and mix and homogenize them for 15 minutes under the conditions of 200W and 15000r / min; In the device, add 60% ethanol solution 5 times the raw material, conduct ultrasonic extraction at 60°C for 100 minutes, concentrate under reduced pressure, recover ethanol, and obtain a concentrated solution; add the obtained concentrated solution to 100-mesh polyamide with the same quality as the raw material In the resin column, after elution of 60% of 5 times the volume of the resin, the eluate was collected, concentrated and dried to obtain the total flavonoids of olive leaves, the extraction rate was 96.3%, and the content of total flavonoids was 90.82% by UV detection.

Embodiment 3

[0021] Dry the olive leaves in the shade, crush them, add 55% ethanol twice as much as the raw materials, place them in a homogenizer, and mix and homogenize them for 15 minutes under the conditions of 200W and 20000r / min; In the device, add a 55% ethanol solution that is 4 times the raw material, perform ultrasonic extraction at 70°C for 120 minutes, concentrate under reduced pressure, recover ethanol, and obtain a concentrated solution; add the obtained concentrated solution to 100-mesh polyamide with the same quality as the raw material In the resin column, after 65% elution of 6 times the volume of the resin, the eluate was collected, concentrated, and dried to obtain the total flavonoids of olive leaves, with an extraction rate of 93.6%. The content of total flavonoids was 93.58% by UV detection.

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Abstract

The invention relates to a preparation method of olive leaf total flavonoids, in particular to a method for extracting olive leaf total flavonoids by adopting a homogenate-ultrasonic method. The method comprises the following treatment steps: drying olive leaves in the shade, crushing, adding 50-60% ethanol which is 1-2 times of the raw materials, and mixing and homogenizing in a homogenizer; after homogenizing, putting the material into an ultrasonic device, adding a 50-60% ethanol solution which is 3-5 times of the raw material, carrying out ultrasonic extraction for 90-120 minutes, carryingout vacuum concentration, and recovering ethanol to obtain a concentrated solution; loading the obtained concentrated solution on a column through 80-100-mesh polyamide resin, eluting, collecting aneluent, concentrating and drying to obtain the olive leaf total flavonoids. The method has the advantages of rapidness, high efficiency, low solvent consumption and the like, the reproducibility is good, and the resin can be repeatedly used.

Description

technical field [0001] The invention relates to a preparation method of total flavonoids of olive leaves, which belongs to the field of extraction of active ingredients of traditional Chinese medicines. Background technique [0002] The total flavonoids of olive leaves can promote blood circulation, improve digestive system function, protect skin, improve endocrine system function, and can be used to prevent cardiovascular and cerebrovascular diseases, anti-aging, radiation protection, and has many beneficial effects. [0003] At present, solvent extraction and microwave synergistic extraction are mostly used for the extraction of total flavonoids. Among the solvent extraction methods, the ethanol extraction method is the most efficient, but the product components are complex and time-consuming; the microwave method saves energy and greatly shortens the time, but the leaked microwave is more likely to cause environmental pollution. The homogenate extraction refers to an ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/63A61K127/00
CPCA61K36/63A61K2236/15A61K2236/333A61K2236/51A61K2236/55
Inventor 叶桂银
Owner 南京艾蒙利特生物科技有限公司
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