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Method for extracting oleuropein from olea europaea leaves

A technology of oleuropein and oleuropein is applied in chemical instruments and methods, pharmaceutical formulations, preparations for skin care, etc., and can solve the problems of low oleuropein content, large consumption of organic solvents, poor extraction effect, and the like. Unique and novel, improve extraction rate, high purity effect

Inactive Publication Date: 2016-10-26
郭舒洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem mainly solved by the present invention: in the process of extracting oleuropein in the current traditional method, the consumption of organic solvent is large, so that the obtained product contains a large amount of organic solvent residues, which cannot be widely used. In addition, oleuropein in the extract The current situation that the content of the content is low and the extraction effect is not good, provides a kind of extracting the pretreated olive leaf powder through the japonica rice and glutinous rice fermentation broth, and adding cellulase and pectinase, so that the active ingredients in the olive leaf powder are fully released , and then centrifuged, concentrated by rotary evaporation and dried, so as to obtain the method of extracting oleuropein from olive leaves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] First weigh 300g of fresh olive leaves, wash them with deionized water for 3 times, remove the residual dirt on the surface, cool them with liquid nitrogen for 3 minutes, take them out quickly, spread them on the stone grinding disc immediately, grind them for 5 minutes, and collect the grinding results The material is transferred into a closed container, and at room temperature, the pressure is reduced to a vacuum of 60kPa, and the pressure is kept for 10 minutes to obtain the pretreated olive leaf powder for subsequent use; then 0.8kg of japonica rice and 3kg of glutinous rice are weighed successively, and after manual mixing , washed 3 times with deionized water, then transferred to a steamer, steamed at 90°C for 60 minutes, then spread it on a bamboo mat, and naturally cooled to 45°C; then transferred the cooled japonica rice and glutinous rice to Put into the fermenter that fills 4L deionized water, add 15g saccharomyces cerevisiae powder again, after stirring 10min...

example 2

[0024] First weigh 400g of fresh olive leaves, wash them with deionized water for 4 times, remove the residual dirt on the surface, cool them with liquid nitrogen for 4 minutes, take them out quickly, spread them on the stone grinding disc immediately, grind them for 8 minutes, and collect the grinding results The material is transferred into a closed container, and at room temperature, the pressure is reduced to a vacuum of 65kPa, and the pressure is maintained for 15 minutes to obtain the pretreated olive leaf powder for subsequent use; then 0.9kg of japonica rice and 4kg of glutinous rice are weighed successively, and after manual mixing , washed 4 times with deionized water, then transferred to a steamer, steamed at 93 °C for 70 min, then spread it on a bamboo mat, and cooled to 50 °C naturally; then transferred the cooled japonica rice and glutinous rice to Put into the fermenter that fills 5L deionized water, add 25g saccharomyces cerevisiae powder again, after stirring 1...

example 3

[0027] First weigh 500g of fresh olive leaves, wash them with deionized water for 5 times, remove the residual dirt on the surface, cool them with liquid nitrogen for 5 minutes, take them out quickly, spread them on the stone grinding disc immediately, grind them for 10 minutes, and collect the grinding results The material is transferred into a closed container, and at room temperature, the pressure is reduced to a vacuum of 70kPa, and the pressure is maintained for 20 minutes to obtain the pretreated olive leaf powder for subsequent use; then 1.0kg of japonica rice and 5kg of glutinous rice are weighed successively, and after manual mixing , washed 5 times with deionized water, then transferred to a steamer, steamed at 95°C for 80 minutes, then spread it on a bamboo mat, and cooled to 55°C naturally; then transferred the cooled japonica rice and glutinous rice to Put into the fermenter that fills 6L deionized water, add 30g saccharomyces cerevisiae powder again, after stirrin...

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Abstract

The invention discloses a method for extracting oleuropein from olea europaea leaves and belongs to the technical field of plant extraction. The method for extracting the oleuropein from the olea europaea leaves comprises the following steps: extracting pre-treated olea europaea leaf powder through polished round-grained rice and polished glutinous rice fermentation liquid; adding cellulase and pectinase to further release effective components in the olea europaea leaf powder; and then centrifugally separating, carrying out rotary evaporation for concentrating and drying to obtain the oleuropein. The embodiment shows that the operation is simple and feasible and the raw materials are simple and easy to obtain; the polished round-grained rice and polished glutinous rice fermentation liquid is used for replacing organic dissolving, so that the obtained oleuropein has no organic solvent residues and is safe and healthy; finally, the purity of the oleuropein extracted from the olea europaea leaves is up to 85% and more and the yield is up to 90% and more; and the oleuropein can be widely applied to production of foods and drugs and is suitable for industrial production.

Description

technical field [0001] The invention discloses a method for extracting oleuropein from olive leaves, belonging to the technical field of plant extraction. Background technique [0002] Oleuropein is a secoiridoid glycoside compound found in at least 25 species of Oleaceae. Oleuropein is another compound with strong biological activity. It has been reported in the literature that it has significant antifungal, anti-inflammatory, antiviral, antioxidative, anticancer and hypoglycemic and other biological activities. In addition, oleuropein also has Antiarrhythmic and antispasmodic effects, it is an angiotensin-converting enzyme inhibitor with good effect; oleuropein can also activate pepsin and reduce the activity of other molds; oleuropein can also reduce the ion-induced Propionate produced by lipid peroxidation. [0003] Oleuropein has the following effects: 1. It can prevent the growth of cells and viruses, and stimulate the activity of important cells in the immune system...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08A61K8/60A61Q19/08A23L33/105
CPCC07H17/04A61K8/602A61Q19/08C07H1/08
Inventor 郭舒洋张明许博伟
Owner 郭舒洋
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