77 results about "Pyrenoid" patented technology

The invention aims to provide a biological preparation method of red nano selenium, which comprises the following steps: by using Chlorella as a biological material and a seleniferous inorganic salt as a selenium source, inoculating the Chlorella into the seleniferous salt culture medium, culturing under lighting conditions, and controlling the culture temperature at 22-28 DEG C to prepare the red nano selenium. In order to overcome the defects in the prior art, the Chlorella used as the culture material is cultured in the seleniferous salt BG-11 culture medium under certain light and temperature conditions to prepare the red nano selenium, thereby lowering the production cost of the nano selenium and developing new medicinal value and health care functions for pyrenoid Chlorella. The method is environment-friendly in the production process, and can not cause environmental pollution.

The invention discloses a nutritional meal replacement powder containing spirulina components, and a production method thereof. The nutritional meal replacement powder mainly comprise spirulina powder, chlorella pyrenoidosa powder, dunaliella salina powder, euglena powder, soybean protein isolate powder, microencapsulated soybean plant oil powder and edible salt. The nutritional meal replacement powder is produced through the steps of crushing, mixing, split packaging and packaging. The nutritional meal replacement powder is produced through compounding the spirulina powder, the chlorella pyrenoidosa powder, the dunaliella salina powder and the euglena powder according to a scientific ratio, and purifying the above mixture, and can be digested and absorbed by human bodies to realize physiologic balance, promote metabolism and enhance immunity. The nutritional meal replacement powder is a comprehensive nutrition set meal containing abundant proteins, chlorophylls, vitamins, mineral matters, calcium, iron, zinc and selenium. The nutritional meal replacement powder is convenient to carry and eat.

The invention discloses a method for cultivating chlorella with methane waste liquor, comprising the following steps: preparing the methane waste liquor into mixed culturing liquid, cultivating pyrenoid chlorella, etc. In the invention, methane waste liquor is used as main material of culturing liquid, thereby remarkably reducing cultivation cost, purifying methane waste liquor, obtaining pyrenoid chlorella with high economic value, and having better economic and social benefits.

The invention discloses an acaiberry health care product for Yin-deficiency constitution. The acaiberry health care product comprises the following raw materials in parts by mass: 1-6 parts of acaiberry, 1-8 parts of chlorella pyrenoidosa, 1-8 parts of loquat leaf, 1-9 parts of the fruit of Chinese wolfberry, 1-10 parts of lily, 1-10 parts of radix polygonati officinalis, 1-12 parts of rhizoma polygonati, 1-5 parts of fingered citron, 1-6 parts of lotus seed, 1-9 parts of spina date seed, 1-5 parts of raspberry, 1-5 parts of emblic leafflower fruit, 1-10 parts of mulberry, 1-6 parts of honey, 0.5-5 parts of cordyceps militaris, 1-5 parts of folium mori, 1-6 parts of fructus alpiniae oxyphyllae, 1-6 parts of the root of kudzu vine, 1-7 parts of horseradish tree leaves and 1-5 parts of Gynura procumbens, and also can comprise 1-9 parts of pawpaw. According to the holism concept of traditional Chinese medicine, yin and yang equilibrium theory and nine constitutions of the human body, the acaiberry serves as a main raw material, the pharmaceutical / food resource raw materials and new food raw materials such as chlorella pyrenoidosa, the fruit of Chinese wolfberry, loquat leaf, lily and radix polygonati officinalis are particularly and elaborately added, and the optimal compatible raw materials are elaborately and scientifically searched, so that the raw materials are complementary with one another, and healthy relation and unique advantages of dietary therapy, herbal nourishment, health maintenance and longevity are fully exerted.

The invention discloses a composite for improving the immunity and application. The composite is prepared from, by weight, 10-30 parts of agaricus blazei murill extract, 10-30 parts of grifola frondosa extract, 10-30 parts of chlorella pyrenoidosa, 5-15 parts of cordyceps militaris extract, 1-10 parts of ginseng extract, 10-20 parts of sea buokthom powder, 1-10 parts of yeast beta-glucose, 1-10 parts of erythritol and 1-5 parts of nutritional yeast powder. It is proved through animal tests that all the raw materials in the composite have an effect on improving the immunity synergistically.

The invention provides a chlorella health beverage and a preparation method thereof and belongs to the technical field of health beverages. The beverage is prepared from a plurality of types of raw materials including wall-broken pyrenoid chlorella liquid, resistant dextrin, xanthan gum, acesulfame, citric acid, D-sodium isoascorbiate, potassium sorbate, green tea extract, corn stigma extract, Chinese angelica extract, fresh tartary buckwheat leaf extract, rock candy, banana polysaccharides and the like; the chlorella health beverage is prepared by extracting active nutritional substances of relative main raw materials through an ultrasonic technology and adding a plurality of types of auxiliary materials. The chlorella health beverage is moderate in manufacturing process, and is safe, environmentally friendly and pollution-free; the rich nutritional substances of the finished product can be easily absorbed by human bodies; the chlorella health beverage has a plurality of types of health effects of enhancing the immunity, resisting ageing, lowering blood lipid, lowering blood pressure, and resisting atherosclerosis and the like after being usually drunk.

The invention relates to the technical field of biological engineering and preparation of clean energy sources, in particular to a preparation method of microalgae biodiesel with high caloric heat. The preparation method comprises the following steps of using chlorella as a raw material to prepare chlorella grease; esterifying the chlorella grease, and centrifugally delaminating, so as to obtain alower biodiesel crude product; continuing to distill, so as to obtain the microalgae biodiesel with high caloric heat. The preparation method has the advantages that the microalgae is promoted to quickly grow under the conditions of relatively moderate light radiation and light quality, highly-concentrated soil extracting solution as culture liquid, proper temperature and the like, so that the diesel production efficiency is improved; the chlorella is single cell pyrenoid chlorella, the pyrenoid chlorella has stable suitability to the environment, the rich nitrogen and phosphor elements and the inorganic elements in biogas slurry can be absorbed, the function of promoting accumulation of chlorella cell grease is realized, the production efficiency of the biodiesel is improved, the maximumcontent of grease is obtained, the content of carbon in the grease is increased, and the purpose of improving the caloric heat of the biodiesel is realized.

The invention discloses chlorella pyrenoidosa, biological environment restoration liquid prepared therefrom and application of the chlorella pyrenoidosa, relates to a green alga active cell biologicalrestoration liquid, and aims to provide chlorella for environment restoration and application of the chlorella. The chlorella is the Chlorella pyrenoidosa ZXL X, and the depositary authority is ChinaCenter for Type Culture Collection, the depositary address is Wuhan University, Wuhan, Hubei Province, the depositary date is November 18, 2020, and the depositary number is CCTCC NO: M 2020760. Thechlorella pyrenoidosa is used for improving the soil environment, promoting crop yield increase and improving crop quality. The restoration liquid is prepared by the following steps: culturing chlorella pyrenoidosa with a nutrient solution to an exponential growth phase and mixing the cultured chlorella pyrenoidosa with an alginate solution; extruding the mixed solution to a calcium chloride solution to obtain globules; and mixing with the globules with a working solution to obtain the biological environment restoration liquid. The environment restoration liquid provided by the invention is high in content value, does not produce drug residues, and can realize agricultural sustainable development.

The invention provides a low-cost, high-biomass and high-protein-content chlorella pyrenoidosa culture method. The method specifically comprises the steps of carrying out straw saccharification on recombinant strains, and carrying out mixotrophic culture on chlorella by taking straw saccharification liquid as a cheap raw material. The method comprises the following steps of: (1) activating recombinant strains of clostridium thermocellum; (2) carrying out straw saccharification: transferring pretreated straws into an anaerobic fermentation system, adding a culture medium and the recombinant strains, and carrying out straw saccharification; (3) preparing a chlorella pyrenoidosa culture solution; and (4) carrying out mixotrophic culture on chlorella pyrenoidosa: inoculating a proper amount ofchlorella species into the culture solution, and carrying out mixotrophic culture under proper light intensity. According to the method, the ability of degrading lignocellulose to produce glucose byclostridium thermocellum fiber bodies is further improved through the recombinant strains of clostridium thermocellum; and the chlorella pyrenoidosa obtained through mixotrophic culture is high in biomass and high in protein content, so that the production cost of chlorella is reduced, and the market competitiveness of chlorella pyrenoidosa and derivative products thereof is improved.

The invention discloses a production technology for high-density culture of chlorella by utilizing a fermentation method. The production technology is characterized in that firstly, chlorella seed culture is carried out through heterotrophic cultivation, in the fourth stage of the heterotrophic cultivation process, a basal culture medium and a fed-batch culture medium are selected and used for co-culture, and the two media are utilized in combination with fermentation tanks to perform high-density heterotrophic cultivation on chlorella pyrenoidosa; besides, by adopting a semi-continuous fed-batch cultivation method and measuring the growth rate, dry weight, protein content and the like of chlorella, the optimum culture condition for maintaining of the stable growth of chlorella strains is determined; the experimental result shows that the pH is 5.5-7.5, DO is larger than or equal to 20%, the best ratio of carbon to nitrogen C / N for feed-batch culture equals to 100, the suitable carbon concentration for feed-batch culture is 40-60 g / L, the reclaiming rate is 1 / 5, chlorella can realize high-density stable growth, and after 4-5 days' fermentation, the dry weight can reach 100 g / L or above, and the protein content can reach 58 g / L or above. The production technology not only realizes pure culture, but also improves the algae quality to a greater extent; besides, chlorella prepared by adopting the production technology contains other multivitamins at the same time, and further meets the nutritional requirements of human bodies.

The invention discloses a method for breeding chickens without using antibiotics. The method comprises the following steps: (1) building a free range; (2) building a henroost; (3) adding 0.05 to 0.1 weight percent of photosynthetic bacteria powder and 0.1 to 0.2 weight percent of chlorella pyrenoidosa powder into basic food; (4) adding 0.5 to 1.0 weight percent of a mixture of chitooligo saccharide powder, aronia melanocarpa powder and the chlorella pyrenoidosa powder into drinking water to implement biotrophic immunity for the chickens; (5) disinfecting a chicken house. According to the method, no antibiotics are used, so that the problem of residual antibiotics caused by delay of time of antibiotics excretion from a chicken body due to the use of a large amount of the antibiotics in theprior art is solved. Meanwhile, the survival rate of the chickens bred by using the method is higher, and the average egg production rate of the chickens is also increased substantially.

The invention relates to a high throughput screening system, screening method and preparation method for a chlorella functional component CPE. The screening method comprises 1) an antioxidant test, 2) a cell immunoregulation test, 3) a redox metal ion chelation test, and 4) an advanced glycation end product AGEs inhibition test. The preparation method comprises adding chlorella pyrenoidosa powder into distilled water, heating the mixture at 110-130 DEG C for 30-60min under high pressure, carrying out extraction, taking out a sample, cooling the sample at the room temperature, carrying out centrifugation at a rotation rate of 10000r for 30min, carrying out vacuum suction filtration on the supernatant, storing the filtrate at 4 DEG C for nest use, adding the same amount of distilled water into the chlorella mud obtained by centrifugation, carrying out resuspension, repeating the above processes, merging the filtrates obtained by the two processes, and carrying out freeze drying to obtain chlorella CPE extract powder. The preparation method has a high yield of the chlorella functional component CPE, and the extract is rich in nucleic acid and proteins. The preparation method is suitable for industrial production, reduces a development cost of microalgae energy and improves the comprehensive utilization degree of microalgae energy.

The invention aims to provide a biological preparation method of red nano selenium, which comprises the following steps: by using Chlorella as a biological material and a seleniferous inorganic salt as a selenium source, inoculating the Chlorella into the seleniferous salt culture medium, culturing under lighting conditions, and controlling the culture temperature at 22-28 DEG C to prepare the red nano selenium. In order to overcome the defects in the prior art, the Chlorella used as the culture material is cultured in the seleniferous salt BG-11 culture medium under certain light and temperature conditions to prepare the red nano selenium, thereby lowering the production cost of the nano selenium and developing new medicinal value and health care functions for pyrenoid Chlorella. The method is environment-friendly in the production process, and can not cause environmental pollution.

The invention discloses a method for realizing efficient hydrogen production based on chlorella cell biomineralization. The invention belongs to the technical field of biological energy, and particularly relates to a method for achieving efficient hydrogen production based on chlorella cell biomineralization. The invention aims to solve the problems that existing biological hydrogen production processes are complicated and high in cost, and cannot realize large-scale hydrogen production. According to the method, chlorella pyrenoidosa is taken as a basic living cell, layer-by-layer self-assembly is carried out on the surface of the chlorella pyrenoidosa based on electrostatic interaction, and finally a functional MOF material which takes GMP as a ligand and Cu<2+> as a metal center and hasnano-enzyme activity is formed. By adding an oxygen consumption substrate, the oxygen concentration of a system is quickly reduced under the catalytic action of MOF, so that efficient and quick hydrogen production is realized. The whole system is easy to construct, mild in process and environmentally friendly, meanwhile, the functionalized MOF shell layer not only serves as a catalytic effect, butalso can maintain the basic activity of cells, and the life cycle of the cells in an unfavorable environment is prolonged. The method is used for biological hydrogen production.

The invention provides a method for accumulating grease mutant chlorella quickly by mutating pyrenoid freshwater chlorella by laser, comprising the following steps: (1) culturing the pyrenoid freshwater chlorella cell to the logarithmic growth phase by using the fluorescent lamp as the light source; (2) taking and pouring 20ml of chlorella solution in the logarithmic growth phase into a small wide-mouth bottle, irradiating by using the semiconductor laser for 0.9-1.1 minutes, and stirring under magnetic force during irradiating to obtain a mutant chlorella; and (3) pouring the irradiated and mutated chlorella solution into a 50ml triangular flask filled with the BG11 nutrition salt with the final concentration of 1 / 4 times, culturing for 20 days at the temperature of 20-25 DEG C and the light intensity of 1,800-2,000lx, transferring the chlorella solution into a 300ml triangular flask, continuing to culture for 20 days, transferring the chlorella solution into a 1,000ml triangular flask, and continuing to culture for 26 days, wherein the BG11 nutrition salt with final concentration of 1 / 4 times is added every eight days, and the chlorella solution is shaken for 3 times every day in the culturing stage. The method is easy and practical, low in cost and high in efficiency.

The invention belongs to the technical field of microalgae biology, and particularly relates to a method for promoting chlorella pyrenoidosa growth by using Volatile fatty acids. According to the present invention, a BG-11 culture medium is used as the base culture medium of chlorella pyrenoidosa, the algae liquid is subjected to centrifugal concentration when the microalgae grows to the logarithmic growth phase, 4-10g / L mixed Volatile Fatty Acids (VFAs) comprising acetic acid, propionic acid and butyric acid are added, the optical density value of the inoculated seed liquid at 680nm is 0.2-1.0, and the obtained material is placed at a temperature of 24-26 DEG C and is cultured for 2-5 days under a dark condition; and with the method, the growth period of heterotrophic chlorella pyrenoidosa can be significantly shortened, the microalgae can obtain the highest biomass concentration within two days, the rapid accumulation of biomass can be achieved, the conversion efficiency can be improved, the high culture cost problem caused by the too long culture period in the actual production can be reduced, and the new idea is provided for the large-scale production of oil production from microalgae.

The invention discloses a preparation method of chlorella pyrenoidosa peptide chelated calcium with antioxidant and calcium absorption promoting activity, and belongs to the technical field of food / agricultural product processing. The method comprises the following steps: carrying out ultrasonic-assisted enzymolysis on chlorella pyrenoidosa, carrying out ultrafiltration grading, carrying out ultrasonic-assisted chelation, and dialyzing, concentrating and drying the mixture so as to obtain the chlorella pyrenoidosa peptide chelated calcium with the activity of resisting oxidation and promoting calcium absorption. The method is simple in process, short in time consumption, low in energy consumption, convenient to operate, safe, free of pollution and capable of being applied to industrial production on a large scale, residual residues of chlorella obtained after grease extraction are used as raw materials, and the bioavailability of chlorella resources is improved; the chlorella pyrenoidosa peptide chelated calcium prepared by the method has double effects of resisting oxidation and improving the bioavailability of mineral calcium, and has important guiding significance on development of nutrient enrichment products and reasonable diet of three meals a day.

The invention discloses a microalgae nutrition repair liquid for relieving grassland desertification, which comprises a microalgae mixed liquid, the microalgae mixed liquid comprises seven active microalgae, and the added microalgae comprises the following components in parts by weight: 5-40 parts of hylothrix tadeeri, 5-40 parts of hylothrix wonderi, 10-50 parts of microcoleus vaginalis, 5-40 parts of pseudocladoidea, 10-50 parts of monofidus microfidus and 30-80 parts of chlorella pyrenoidosa, and the microalgae mixed liquid comprises the following components in parts by weight: 5-40 parts of hylothrix tadeeri, 5-40 parts of hylothrix wonderi, 10-50 parts of microcoleus vaginalis and 30-80 parts of chlorella pyrenoidosa. In addition, 10-50 parts of nitrogen-fixing anabaena or 20 parts of cotton algae are selected to be added. Aiming at the grassland desertification region, the nutrient restoration liquid can restore soil, supplement natural nitrogen, activate soil trace elements, improve plant stress resistance and prevent plant diseases and insect pests, the seven microalgae have good compatibility, the number of microalgae active cells in each 1mL of the nutrient restoration liquid can reach more than 106, and the nutrient restoration liquid has good application prospects. The storage life of the nutrient repair liquid reaches 18 months, the culture process is obtained through a large number of experiments, and the culture efficiency is high.

The invention provides a moisturizing and brightening moisturizing essence. The moisturizing and brightening moisturizing essence is characterized by being prepared from, by mass, 0.6% of seaweed extract, 0.6% of cod liver oil, 0.5% of sea turtle oil, 0. 135% of coral powder, 0.385% of rhopilema esculentum extract, 0.5% of crithmum maritimum extract, 0.6% of chlorella pyrenoidosa extract, 3% of robinia pseudoacacia extract, 2% of beeswax, 4.55% of coconut oil, 3.5% of glycerin, 5.0% of soya bean lecithin, 2.0% of sandalwood essential oil, 3.0% of prunus almond oil, 2.0% of honey, 1.0% of oliveoil and 0.6% of wheat oil. The moisturizing essence using natural materials can have roughly same technical effects as the moisturizing essence prepared by a synthetic material and avoids the damageof the synthetic material on the human body; and the production process cannot pollute the environment.

The invention relates to the technical field of chlorella pyrenoidosa, and provides a method for fluorescence detection of biotoxicity of atrazine by using chlorella pyrenoidosa, the biotoxicity of atrazine is detected by using chlorella pyrenoidosa under the condition of vegetable oil, the vegetable oil is methylated and ethylated vegetable oil, and the content of the methylated and ethylated vegetable oil is less than 1%. Comprising the following steps: taking atrazine standard solutions with a series of concentrations, adding a vegetable oil solution, then adding into a chlorella pyrenoidosa solution, uniformly mixing, controlling the volume ratio of the chlorella pyrenoidosa solution to the vegetable oil solution to the atrazine standard solution to be 1: (1-2): (1-2), darkly placing for 10 minutes, measuring each chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa, and calculating the chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa according to the chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa. And determining the biotoxicity of atrazine. According to the technical scheme, the problem that a method for detecting the biotoxicity of atrazine in the prior art is long in determination period is solved.

The present invention discloses Veisseria sp. cultured by sea water, namely a Veisseria sp. LQ-1984 strain. The Veisseria sp. LQ-1984 strain has a number of LQ-1984, has a Latin name of Vischeia sp.,has an accession number of CCTCC M 20191120, and is named as Vischeia sp. LQ-1984. Mature cells of the Veisseria sp. LQ-1984 has a smooth surface, has no protrusion in the cell wall, has a diameter of10-15 microns, is of a spherical shape or a nearly spherical shape, has chlorophyll with a lobate shape, and contains a protein nucleus. Compared with other omega-7 fatty acid production raw materials, the Veisseria sp. LQ-1984 strain is not limited by environments, regions, seasons and the like, and can meet huge raw material gaps. The Veisseria sp. LQ-1984 strain contains high-content of omega-7 fatty acid, and high-quality algae powder of the Veisseria sp. LQ-1984 strain can be used for producing omega-7 related health care products, foods, cosmetics and the like.

The invention discloses a method for determining the influence of pollutants on the content of active oxygen in green alga cells. Chlorella pyrenoidosa is used for evaluating the influence of a typical pollutant azole bactericide (clotrimazole) on the content of active oxygen in green alga cells; and evaluating the influence of pollutants on active oxygen in the green alga cells according to the DCF fluorescence intensity of the single green alga cells under the action of the azole bactericide pesticide. According to the method, the detection result of the change of the content of the active oxygen in the green alga cells is more accurate, and the problem that the fluorescence intensity measurement is inaccurate due to the change of the number of the cells caused by exposure of pollutantsat different concentrations is solved. The fluorescence intensity of DCF is in direct proportion to the content of active oxygen in cells, so that the influence of pollutants on the content of activeoxygen in cells under a certain concentration can be reflected more intuitively. The method is simple, convenient and rapid in test, low in detection system background, high in sensitivity and good inrepeatability.

The invention discloses a method for treating rural domestic sewage by using chlorella pyrenoidosa, and relates to a treatment method of rural domestic sewage. The method aims at solving the technical problem that an existing method for treating rural sewage by utilizing an algae pond is poor in pollutant removal effect. The method comprises the following steps: 1, culturing chlorella pyrenoidosa; 2, domesticating the chlorella pyrenoidosa; and 3, inoculating the secondary domesticated algae liquid into the rural domestic sewage with the algae concentration of 105-106 cell / mL, and treating for 8-10 days under the conditions of the temperature of 25 + / -1 DEG C, the illumination intensity of 100 [mu] mol / (m<2>.S) and aeration to complete the treatment of the rural domestic sewage. According to the invention, the rural domestic sewage is treated by taking photosynthesis of chlorella pyrenoidosa as a main carrier, and the effluent quality meets the first-grade B discharge standard of sewage. The method can be used in the field of rural domestic sewage treatment.

The invention relates to a preparation method of a three-dimensional porous algae-based / chitosan aerogel and application of the three-dimensional porous algae-based / chitosan aerogel in treatment of uranium-bearing wastewater. The preparation method of the three-dimensional porous algae-based / chitosan aerogel used for uranium-bearing wastewater treatment comprises the following steps: taking chitosan and chlorella pyrenoidosa as base materials, utilizing ammonium bicarbonate and sodium bicarbonate to foam inside the aerogel and form holes, and then carrying out low temperature refrigeration andsolvent replacement to prepare the three-dimensional porous algae-based / chitosan aerogel. The three-dimensional porous algae-based / chitosan aerogel prepared in the invention is large in specific surface area and controllable in volume, can be separated from a solution easily, and has high mechanical strength. The adsorption and saturation capacity of three-dimensional porous algae-based / chitosanaerogel for uranium reaches 575 mg / g, the removal rate for uranium below 100 mg / L reaches up to 95% or more, and the three-dimensional porous algae-based / chitosan aerogel can be applied to enrichmentand removal of uranium in the water solution.

The invention discloses a preparation method of chlorella pyrenoidosa polyphenol. The chlorella pyrenoidosa polyphenol is extracted by using the preparation method comprising the following steps of smashing, mixing, extracting and filtering. The preparation method has the advantages of high absorption rate, low cost, convenience for industrial production and the like.

The invention discloses a dietary composition for cancer dietary intervention and a preparation method thereof. The dietary composition comprises the following components in percentage by weight: 40-60% of tea pigment, 10-20% of an agaricus blazei murill extract, 8-12% of a grifola frondosa extract, 4-6% of a dandelion extract and 15-25% of a chlorella pyrenoidosa extract. The preparation method comprises the following steps: S1, preparing tea pigment powder for later use; S2, preparing the agaricus blazei murill extract powder for later use; S3, preparing the grifola frondosa extract powder for later use; S4, preparing dandelion extract powder for later use; S5, preparing the chlorella pyrenoidosa extract powder for standby application; and S6, uniformly mixing the tea pigment powder, theagaricus blazei murill extract powder, the grifola frondosa extract powder, the dandelion extract powder and the chlorella pyrenoidosa extract powder obtained in the steps S1, S2, S3, S4 and S5 in percentage by weight to obtain the dietary composition. The dietary composition disclosed by the invention has an excellent body conditioning effect.

The invention discloses prebiotic soft sweets and a preparation method thereof. The prebiotic soft sweets are prepared from the following raw materials: fructooligosaccharide syrup, galactooligosaccharide syrup, carrageenan, chlorella pyrenoidosa, glutinous rice flour, haematococcus pluvialis, lutein lipid, collagen, probiotics and water. The preparation method comprises the following steps: S1, adding the fructo-oligosaccharide syrup and the galacto-oligosaccharide syrup into water, uniformly stirring, and heating to obtain a first mixture; S2, preparing a carrageenan solution, adding the carrageenan solution into the first mixture, uniformly stirring, and continuously heating to obtain a third mixture; S3, preparing a chlorella pyrenoidosa mixed solution, adding the chlorella pyrenoidosa mixed solution into the third mixture, and uniformly stirring to obtain a fourth mixture; S4, sieving the fourth mixture to obtain a fifth mixture; S5, injecting the fifth mixture into a mold for molding; S6, after the fifth mixture is cooled, smearing a layer of glutinous rice flour on the surface of the fifth mixture, and obtaining formed soft sweets; and S7, dehumidifying and drying the formed soft sweets to obtain the prebiotic soft sweets.

The invention provides a culture method facilitating the increase of the growth rate of chlorella pyrenoidosa and cell density and the promotion of fat accumulation. Mixed bacterial fermentation liquid with inactivated Rhodobacter sp. and Rhodopseudomonas sp. is added in a culture medium for culturing chlorella pyrenoidosa. Through the adoption of the culture method, chlorella pyrenoidosa cells can grow quickly, and cultured chlorella pyrenoidosa is high in cell intensity; in particular, by adding the mixed bacterial liquid, the quick multiplication of the chlorella pyrenoidosa cells is promoted, the concentration of the chlorella pyrenoidosa cells is increased, the content of fat is obviously increased, and the method is suitable for mass and large-scale culture production.

The invention relates to the technical field of toothpaste and a preparation method thereof, in particular to tianshan cold water algae functional toothpaste and a preparation method thereof. The toothpaste comprises the following raw materials of chlorella pyrenoidosa nano superfine powder, calcium carbonate, xylitol, hydrated silica, glycerinum, methylparaben, sodium lauryl sulfate, tetrasodiumpyrophosphate, cellulose gum xanthan gum, sodium benzoate, edible essence and reverse osmosis secondary purified water, xylitol and glycerinum are added into the reverse osmosis secondary purified water to obtain first slurry, the first slurry is mixed with chlorella pyrenoidosa nano superfine powder, calcium carbonate, tetrasodium pyrophosphate, cellulose gum xanthan gum, methylparaben, sodium lauryl sulfate, hydrated silica and sodium benzoate to obtain second slurry, and the second slurry is emulsified with the essence dissolving solution to obtain the tianshan cold water algae functional toothpaste. The preparation method is simple and convenient; and the obtained tianshan cold water algae functional toothpaste can maintain gingiva, relieve gingival bleeding, remove bad breath, and prevent periodontitis, and then the comprehensive health of the oral cavity is realized.

The invention relates to a heterotrophism-dilution-light induction culture method for desert chlorella pyrenoidosa. According to the method, a heterotrophic mode and an autotrophic mode are combined, so that the biomass and the protein content of the desert chlorella pyrenoidosa are greatly improved, the purpose of efficiently producing a large amount of desert chlorella pyrenoidosa with high protein content is achieved, the chlorella pyrenoidosa cell biomass with relatively high concentration can be obtained in a short time, the accumulation of substances such as protein and chlorophyll in the chlorella pyrenoidosa can be induced, the quality of the chlorella pyrenoidosa is improved, and the comprehensive utilization value of the chlorella pyrenoidosa is increased. Besides, the method has the characteristics of simplicity and convenience in operation, high practicability, easiness in realization of continuous production and the like, and is very beneficial to large-scale popularization and application, so that a large amount of desert chlorella pyrenoidosa powder with high protein content can be quickly and efficiently obtained, the technical support is provided for production of the high-quality desert chlorella pyrenoidosa powder, and the requirements of industrial production are met.