42 results about "Pyrenoid" patented technology

The invention discloses a preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight. The novel preparation method of the microalgae nano-selenium comprises the following steps: firstly, extracting an intracellular extract of chlorella pyrenoidosa, and then adding sodium selenite into the intracellular extract to carry out nano-selenium conversion; compared with an existing reported method that after sodium selenite is added into a microalgae living body culture solution to be converted into nano-selenium, algae is taken to break cells, and the nano-selenium is collected, the method has the advantages that the microalgae living body does not need to be used when the nano-selenium is biologically converted, and the subsequent complicated nano-selenium separation and purification steps can be avoided; the nano-selenium obtained by converting the intracellular extract can be directly applied. Experiments prove that the effect of the nano-selenium obtained through biotransformation on inhibiting phytophthora infestans of potato late blight pathogenic bacteria is obviously better than that of chemically synthesized nano-selenium, and the nano-selenium obtained through biotransformation has an obvious effect on preventing and treating potato late blight and has an obvious effect on promoting plant growth.

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.

The invention provides a chlorella pyrenoidosa calcium chelating peptide and a chlorella pyrenoidosa peptide calcium chelate prepared by using the chlorella pyrenoidosa calcium chelating peptide. The prepared chlorella pyrenoidosa peptide calcium chelate can enhance the maximum load and structural strength of the tibia of a mouse with osteoporosis. The chlorella pyrenoidosa calcium chelating peptide provided by the invention is combined with calcium ions to form a peptide-calcium chelate, and animal experiments prove that the peptide-calcium chelate can significantly improve the maximum load and structural strength of the tibia of a mouse with osteoporosis, and has a good effect of improving the bone biomechanical properties of the mouse with osteoporosis.

The invention discloses a chlorella pyrenoidosa white mutant strain FZU65-W1 and application of the chlorella pyrenoidosa white mutant The classification name of the chlorella pyrenoidosa white mutant strain FZU65-W1 is chlorella pyrenoidosa, the chlorella pyrenoidosa white mutant strain is preserved in the China General Microbiological Culture Collection Center on September 11, 2024, the preservation number is CGMCC N0.46151, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain FZU65, the chlorella pyrenoidosa white mutant strain FZU65-W1 has the advantages that the growth speed of algae cells under the heterotrophic condition is higher, and meanwhile, the cells are white due to the fact that chlorophyll and carotenoid are not synthesized in the cells. The chlorella pyrenoidosa white mutant strain FZU65-W1 can be used for producing high-quality protein through heterotrophic fermentation, so that not only can a later-stage protein extraction and preparation process be simplified, but also the sensory acceptance of algae powder can be improved, and a high-quality germplasm resource is provided for industrial production and application of microalgae protein.

The invention relates to the technical field of microalgae heterotrophic culture and biomass proliferation, in particular to a chlorella pyrenoidosa heterotrophic high-density proliferation method. The specific technical scheme is as follows: the heterotrophic high-density proliferation method of chlorella pyrenoidosa comprises the following steps: pre-culture and heterotrophic propagation of chlorella pyrenoidosa: inoculating a chlorella pyrenoidosa solution into a BG11 culture medium, and culturing for 3-4 days in a constant-temperature shaking table with the temperature of 25 + / -1 DEG C, the rotating speed of 200rpm and the light-dark ratio of 0h: 24h to obtain a primary culture chlorella solution; inoculating the primary culture algae liquid into a pre-sterilized and optimized heterotrophic culture medium, and carrying out expanding culture under the same culture condition for 3-4 days to obtain a secondary expanding culture algae liquid; (2) a heterotrophic high-density culture stage: regularly supplementing a nutrient culture medium containing high-concentration glucose, nitrate and phosphate by adopting an index fed-batch feeding strategy; other conditions are kept consistent during culture; and (3) an in-situ protein accumulation induction stage: when the chlorella pyrenoidosa reaches an exponential growth end stage, adding paclobutrazol into the system, and keeping other conditions consistent while keeping exponential fed-batch culture. According to the method disclosed by the invention, the biomass of the chlorella pyrenoidosa is increased by about 128.6% compared with that of the traditional batch culture, the protein content can reach 49.14%, meanwhile, the configuration of essential amino acids is improved, and the requirements of high-end biomass resource application are met. The method has the advantages of simplicity and convenience in operation and high production efficiency, and is suitable for the fields of high-quality protein extraction and related biomass production.

According to the chlorella pyrenoidosa high-F-value peptide and the preparation method thereof, parameters in the enzymolysis and adsorption processes are strictly controlled, and the enzymolysis of the incision enzyme and the excision enzyme not only can efficiently hydrolyze chlorella protein and generate more peptide fragments with biological activity, but also can be used for preparing the chlorella pyrenoidosa high-F-value peptide. And the characteristics of high industrial production cost and poor stability caused by wide enzyme cutting sites are solved. The prepared chlorella pyrenoidosa high-F-value peptide reaches 36.90 + / -8.18, the F value of the chlorella pyrenoidosa high-F-value peptide is increased by 12.72 times compared with the chlorella pyrenoidosa raw material (2.90 + / -0.07), and in addition, the molecular weight of the high-F-value oligopeptide is 1t; peptides with the molecular weight of 1000 Da account for 95-99.40%, the characteristics of oligopeptides are met, the molecular weight is small, and digestion and absorption are easy. The prepared high-F-value oligopeptide can accelerate metabolism of ethyl alcohol in the liver and protect the liver from being damaged by the ethyl alcohol, and has the effects of dispelling the effects of alcohol and protecting the liver.

The invention provides a method for regulating and controlling growth and metabolism of caproic acid bacteria by using marine active peptides in a baijiu fermentation process. According to the method, the marine active peptides are added in an anaerobic fermentation stage of baijiu preparation to regulate and control the growth and metabolism of the caproic acid bacteria; wherein the marine active peptide is chlorella pyrenoidosa peptide, schizochytrium limacinum peptide, fish albumin peptide or euphausia superba peptide. According to the method, active peptides with specific sources are added into an anaerobic culture system, so that the growth of caproic acid bacteria can be promoted, energy metabolism can be enhanced, and the activity of key enzymes such as caproic acid dehydrogenase and caproic acid synthase can be activated, and therefore, the generation amount of flavor acids such as caproic acid and butyric acid can be increased, the fermentation quality can be improved, and the flavor level and esterification precursor substance content of Baijiu can be improved.

This invention belongs to the field of functional food processing technology, specifically relating to a method for improving the flavor of a chlorophyll-deficient Chlorella proteoglycan mutant. The method includes the following steps: S1, obtaining a Chlorella proteoglycan mutant strain; S2, centrifuging the Chlorella proteoglycan mutant strain and collecting the algal sludge, then pulverizing the collected algal sludge and dispersing it in water to obtain an algal powder suspension, then adding a compound enzyme to the algal powder suspension, and inactivating the enzyme after the reaction; S3, cooling the system to room temperature, adding glucose and stirring to dissolve, sterilizing, inoculating with a compound bacterial strain and fermenting, and sterilizing again after fermentation; S4, cooling the fermented system to room temperature, then adding β-cyclodextrin and stirring, and drying the system after stirring to obtain the algal protein product. This invention employs a synergistic process of compound enzymatic hydrolysis, compound bacterial fermentation, and β-cyclodextrin encapsulation to remove the off-flavor of the mutant algae from the source, significantly improving the flavor and enhancing the product's storage stability.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

The invention provides a method for producing an extracellular polymeric substance by using an algal-bacterial symbiotic system, and belongs to the technical field of industrial biology. The method for producing the extracellular polymeric substance by utilizing the algal-bacterial symbiotic system comprises the following steps: inoculating chlorella pyrenoidosa and lactobacillus paracasei into a BG11 liquid culture medium, culturing under the conditions that the temperature is 27-29 DEG C, the rotating speed is 160-200rpm, the illumination intensity is 3800-4200LX and the illumination ratio is 24h: 0h, then respectively collecting supernate 1 and thalli, resuspending the thalli, collecting supernate 2, and carrying out freeze-drying to obtain the extracellular polymeric substance. Enabling the supernatant 1 and the supernatant 2 to pass through a filter membrane respectively, and collecting filtrate to obtain the traditional Chinese medicine composition. When the concentration of glucose in the G11 liquid culture medium is 3.8-4.2 g / L, the pH value is 4.7-5.2, and the ratio of bacteria to algae is (0.8-1.2): 1, the growth condition of the bacteria-algae symbiotic system is optimal, and when the concentration of sodium selenite is 2-4 mg / L, the yield of the extracellular polymeric substance is highest, and efficient preparation of the extracellular polymeric substance is realized.

The present application relates to a kind of illumination culture methods for improving chlorella pyrenoidosa biomass, the illumination culture method includes the following steps: chlorella pyrenoidosa stock solution is inoculated in sterile liquid medium, and chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light.The present application develops a kind of culture method for chlorella pyrenoidosa, i.e.chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light, the culture method is simple to operate, easy to implement, the method can significantly improve the growth rate and biomass accumulation of chlorella pyrenoidosa, realize the high-quality large-scale production of chlorella pyrenoidosa;It can also reduce the production energy consumption per unit biomass, solves the technical problem that yield increase and consumption reduction are difficult to consider in microalgae culture.

The invention provides a skin care composition based on a mixed chlorella extract as well as a preparation method and application of the skin care composition, and belongs to the technical field of cosmetics. The chlorella essence is prepared from the following raw material components in percentage by weight: 7%-15% of a mixed chlorella extract, 4%-10% of a synergist, 12%-30% of a humectant, 2.0%-8.0% of an emulsifier, 0.3%-0.6% of a thickening agent, 0.5%-1.2% of a preservative, 0.01%-0.4% of perfume and the balance of deionized water, the mixed chlorella extract is obtained by extracting chlorella pyrenoidosa, chlorella vulgaris and chlorella ellipsosa as raw materials. The chlorella pyrenoidosa, the chlorella vulgaris and the chlorella ellipsosa are used as raw materials to prepare the mixed chlorella extract, multiple effects and synergistic effects of the mixed chlorella extract are fully exerted, and the skin moisturizing and repairing effects are remarkably improved.

A method for improving hydrogen production efficiency by using Chlorella motors, belonging to the field of bioenergy technology. The present invention is to solve the problems that after the formation of Chlorella aggregates, it is difficult to redisperse them, resulting in a decrease in the acquisition rate of external electrons by internal Chlorella, a decrease in light energy utilization rate, and a decrease in its own survival rate, etc. The method is as follows: Using Chlorella pyrenoidosa as the living motor matrix, PDA-modified Ti3C2 two-dimensional material as the main shell material, and titanium dioxide (TiO2) nanomaterial as the motor driving material, a light-driven Chlorella motor with a yin-yang structure is constructed. By using its controllable "dispersion-aggregation" cyclic behavior, the effect of "accumulating electrons for hydrogen production when dispersed - consuming electrons for efficient hydrogen production when aggregated" of Chlorella is realized, thereby improving the survival rate, nutrient utilization rate and hydrogen production efficiency. The construction of this system is simple and the process is mild. The Ti3C2 shell has a protective effect on the biological activity and function of Chlorella, and the TiO2 shell has a photocatalytic function and a protective function.

The invention provides a process for producing an indoleacetic acid organic fertilizer by utilizing microalgae, which comprises the following steps: A, putting pig manure, chlorella pyrenoidosa solution and hermetia illucens larvae into a treatment pond, and uniformly stirring until the concentration of the chlorella pyrenoidosa solution is not lower than 1 * 10 < 8 > / L; the addition amount of the chlorella pyrenoidosa solution is 10-30 liters per ton of pig manure, and the addition amount of the hermetia illucens larvae is 600,000-120,000 per ton of pig manure; b, pig manure and chlorella pyrenoidosa liquid are added into the treatment pond according to a plurality of preset time; c, separating hermetia illucens larvae and insect sand within the preset time after the pig manure and the chlorella pyrenoidosa liquid are added for the last time, and treating the insect sand to obtain the organic fertilizer containing indoleacetic acid. According to the invention, by introducing chlorella, the generation of indoleacetic acid in pig manure insect sand treated by hermetia illucens is promoted, meanwhile, the content of nitrogen, phosphorus and potassium elements in the insect sand is increased, and a solid foundation is laid for the use effect of plant fertilizers of the insect sand.

The invention discloses a plant lactobacillus and an application of the plant lactobacillus in a chlorella pyrenoidosa fermented beverage. According to the invention, a strain of phytobacterium plantarum is separated and screened from an outdoor chlorella pyrenoidosa culture pond, is named as phytobacterium plantarum HN3, and is preserved in Guangdong Microbial Culture Collection Center on the fifth floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City on July 26, 2024, and the preservation number is GDMCC No: 64816. The plant lactobacillus HN3 has good fermentation performance, acid tolerance and cholate tolerance, can degrade unpleasant odor substances of chlorella pyrenoidosa liquid to generate new pleasant odor substances, and can be used as a production strain of a chlorella pyrenoidosa fermented beverage.

The invention discloses a method for screening a chlorella pyrenoidosa mutant, which comprises the following steps: carrying out activation, culture and mutagenesis on chlorella pyrenoidosa to obtain a chlorella pyrenoidosa solution; wrapping the mutagenized algae liquid in micro-droplets, and collecting the micro-droplets in a conduit; culturing the wrapped micro-droplets under a given condition; and detecting a fluorescence signal of the cultured micro-droplet, and screening out chlorella pyrenoidosa with fast and slow growth rate based on the intensity of the fluorescence signal. The method has the beneficial effects of high flux, high precision and the like, and overcomes the defects of low flux and high false positive of the traditional method.

The invention discloses a microbial remediation agent for heavy metal remediation as well as a preparation method and application of the microbial remediation agent. The microbial remediation agent is prepared by loading pseudomonas aeruginosa onto a carrier, the carrier comprises the following components in parts by weight: 8-22 parts of chlorella pyrenoidosa solution, 1-3 parts of silicon fertilizer and 1-3 parts of nano zero-valent iron; the silicon fertilizer is obtained by mixing granite stone powder, marble powder and an alkali activator according to the mass ratio of (8-12): (0-12): (0.5-4) and then calcining; the alkali activator is solid sodium hydroxide or potassium hydroxide. The microbial remediation agent disclosed by the invention is high in activity, rapid in reproduction, environment-friendly, pollution-free and low in cost; the form of the heavy metal can be changed, and the high-toxicity form of the heavy metal is converted into the low-toxicity form, so that the toxicity and mobility of the heavy metal are reduced, and the pollution caused by the heavy metal is remarkably reduced.

The invention discloses application of chlorella pyrenoidosa as a feed additive in Hu sheep feed preparation and breeding, and relates to the technical field of ruminant nutrition and feed. The invention provides a ruminant animal feed based on chlorella pyrenoidosa and with chlorella pyrenoidosa powder as a feed additive (the content is 1-5%). The chlorella pyrenoidosa powder contains more than or equal to 55% of crude protein and more than or equal to 25% of polyunsaturated fatty acid; the used basic ration comprises more than or equal to 14% of crude protein, more than or equal to 15.0% of crude fiber, more than or equal to 16.0% of crude ash, more than or equal to 0.50% of calcium, more than or equal to 0.40% of total phosphorus, 0.30-2.00% of sodium chloride, more than or equal to 0.40% of lysine and more than or equal to 14.0% of water. The traditional Chinese medicine composition has the effects of improving lipid metabolism and reducing deposition of saturated fatty acid for Hu sheep; the oxidation resistance and the immunity are enhanced; the meat color index is optimized, and the commodity value is improved. In addition, the feed has the advantages of reducing feed cost, adapting to efficient utilization of desert resources and the like.

The invention relates to the technical field of food, in particular to microalgae emulsion and a preparation method thereof. The microalgae emulsion provided by the invention comprises chlorella pyrenoidosa subjected to green removal; and a liquid phase for dispersing the chlorella pyrenoidosa, wherein the liquid phase consists of water and corn oil. The chlorophyll content of the chlorella pyrenoidosa subjected to green removal is extremely low, meanwhile, the protein content of the chlorella pyrenoidosa subjected to green removal is extremely high, and the formed microalgae emulsion can be milky white, so that the adverse effect of conventional green chlorella on food sense organs can be reduced, and meanwhile, the green chlorella pyrenoidosa subjected to green removal is prepared based on the effect of a liquid phase composed of water and corn oil and the chlorella pyrenoidosa subjected to green removal. The microalgae emulsion disclosed by the invention also has good stability; therefore, the microalgae emulsion disclosed by the invention has very good stability and consumer acceptability, and has a very good application prospect in the field of foods.

The invention relates to the field of microalgae breeding, in particular to a method for screening and cultivating yellow chlorella pyrenoidosa mutant strains capable of rapidly growing, which mainly comprises the following steps: marking wild type chlorella pyrenoidosa on a flat plate, selecting single algae colonies for activation culture, and culturing the single algae colonies to obtain the yellow chlorella pyrenoidosa mutant strains. The yellow chlorella pyrenoidosa capable of rapidly growing is screened and cultured, the yellow chlorella pyrenoidosa is cultured for 4-6 days under the heterotrophic condition of 25-30 DEG C, the color characterization is stable, the hereditary character of the yellow chlorella pyrenoidosa can be judged to be relatively stable, the growth rate is high, the chlorophyll content is remarkably lower than that of wild type chlorella pyrenoidosa, the biomass of the next day can reach 2.1 g / L under the condition that the optimal carbon source is 5g / L, and the yield is high. Compared with wild type chlorella pyrenoidosa, the invention has the advantages that the content of xanthophyll and biomass are higher than those of wild type chlorella pyrenoidosa, the amino acid composition is excellent, the EAA / TAA value is 44.74%, and the EAA / NEAA ratio is 0.81, so that the chlorella pyrenoidosa has the potential of replacing traditional edible resources, can be used as an optimal substitute of traditional food due to the excellent protein quality, and broadens the application market of chlorella pyrenoidosa.

The invention discloses an active desert microalgae biological fertilizer and application thereof, and belongs to the technical field of biological fertilizers. The active desert microalgae biological fertilizer is prepared from the following components in percentage by weight: 10 to 50 percent of cyanobacteria nitrogen fixation, 10 to 30 percent of microcoleus vasinatus, 10 to 50 percent of chlorella pyrenoidosa, 10 to 30 percent of Scenedesmus armatus, 10 to 50 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens, 10 to 30 percent of bacillus amyloliquefaciens and 10 to 30 percent of bacillus The microalgae in the desert microalgae biological fertilizer is in a living body state, can perform a series of life activities such as cell reproduction and metabolism, is vigorous in vitality and high in carbon fixation and nitrogen fixation efficiency, and can remarkably improve the soil fertility, provide nutrition for crop growth, prevent soil hardening and improve the stress resistance of crops.

The invention relates to a white chlorella pyrenoidosa mutant strain and application thereof, and belongs to the technical field of microalgae biology. The chlorella pyrenoidosa mutant strain is named as YYZM024, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.40787, and the preservation date is September 19, 2023. The mutant strain is cultured in a glucose-containing heterotrophic culture medium, and after the mutant strain is cultured under the dark condition of 25-28 DEG C for 7-10 days, the content of chlorophyll in cells is still stably maintained at a relatively low level, and the color characterization is very stable. The chlorophyll content of the mutant strain is not obviously changed after passage inoculation for six months, and dry algae powder is always milky white, so that the genetic character of the mutant strain is relatively stable, and the mutant strain can be used as an algae species for large-scale production of algae source protein or an algae powder source of food additives.

The application belongs to the technical field of soil remediation, and discloses a microalgae biological soil remediation agent and application thereof.A sustainable benign underground micro-ecosystem is constructed, and the prepared microalgae biological soil remediation agent takes a porous active carrier with positive charges as a matrix.The porous active carrier is obtained by compounding silicon dioxide on montmorillonite, and the porous active carrier is modified to obtain an amine group of a negative charge NH2 ‑ between layers, and finally loaded with chitosan cations to become a porous active carrier with positive charges, which is loaded with a large amount of Chlorella pyrenoidosa with reduced environmental sensitivity, so that it can survive and reproduce in harsh soil environments, can significantly improve soil fertility, improve crop yield and quality, and also has the effect of reducing soil heavy metal pollution; can also increase the diversity of soil microbial communities, improve soil aggregate structure, and improve the soil physical and chemical environment.

The invention discloses a method for producing high-organic-calcium microalgae single-cell protein by recycling white spirit vinasse, which comprises the following steps: by taking the white spirit vinasse as a nutrient medium and chlorella pyrenoidosa as a single-cell protein source, firstly, pretreating the white spirit vinasse and carrying out microbial acid-producing fermentation, and dissolving the organic acid generated by fermentation into low-cost inorganic calcium (calcium carbonate) in a system to obtain the high-organic-calcium microalgae single-cell protein. When the pH of the system is adjusted and the acid inhibition in the fermentation process is relieved, an available calcium source and an organic acid carbon source are provided for the chlorella pyrenoidosa; then carrying out directional domestication breeding on the chlorella pyrenoidosa to improve the tolerance, the growth efficiency and the calcium enrichment rate of the chlorella pyrenoidosa; and finally, culturing the domesticated chlorella pyrenoidosa by using a vinasse culture medium subjected to fermentation conversion to produce microalgae single-cell protein rich in organic calcium. According to the invention, the resource reutilization of food wastes is realized, the microalgae culture cost is reduced, the organic calcium content in the obtained single-cell protein product is increased (greater than or equal to 0.48%), and the single-cell protein product can be used for preparing functional foods.

The invention discloses a composition containing agilawood extract and a preparation method and application thereof, and belongs to the technical field of biology, the composition comprises 5-20 parts of agilawood extract, 20-60 parts of functional peptide and 5-25 parts of seaweed functional component; wherein the seaweed functional component is used for forming an embedding, gel or slow-release structure; the functional peptide is preferably chlorella pyrenoidosa peptide and is prepared by combining freeze-thaw ultrasonic-assisted enzymolysis with cation exchange chromatography; the agilawood extract is preferably an agilawood chromone enriched extract; during preparation, the seaweed functional component is dissolved in water, the agilawood extract is added for dispersion or emulsification, then the functional peptide is added for uniform mixing, and freeze-drying, spray-drying or forming treatment is performed to obtain the seaweed functional peptide. The composition can effectively improve the stability and bioavailability of active ingredients, and has a good application prospect in preparation of products for auxiliary protection of acute, alcoholic, drug-induced or fatty liver injuries.

The invention relates to the technical field of microalgae biology, in particular to a chlorella pyrenoidosa mutant strain and application thereof. According to the invention, a mutagenesis screening mode is adopted to obtain a chlorella pyrenoidosa mutant strain which is named as RMCP012, the chlorella pyrenoidosa mutant strain can still grow quickly after being continuously cultured for one year and is stable in character, and the protein content of the chlorella pyrenoidosa mutant strain is equivalent to that of a wild type chlorella pyrenoidosa mutant strain. Meanwhile, the algal strain of the mutant strain is light green, the decoloring process of industrial production of algal source protein is greatly reduced, the industrial cost is reduced, and the mutant strain is a high-quality algal strain capable of being used for industrial production of protein. The invention further relates to a heterotrophic culture mode of the chlorella pyrenoidosa, so that the chlorella pyrenoidosa can grow quickly, and protein with a relatively high level can be accumulated.

The application discloses a method for extracting and separating high-carbon and low-nitrogen and phosphorus organic components in blue algae. The method adopts a process of step-by-step extraction by using an alkaline solution, and when necessary, a mild wall breaking is assisted by adding a low-concentration oxidizing agent, and the interaction between biological molecules is weakened by using sodium chloride, so that high-carbon organic components such as polysaccharides and lipids are effectively decomposed and extracted, and the outflow of high-nitrogen and high-phosphorus components such as proteins and nucleic acids is largely prevented, and the high-carbon and low-nitrogen and phosphorus organic components in the blue algae are successfully obtained. The reagents used in the application are cheap, safe and environmentally friendly, the operation condition is mild, the energy consumption is low, the application is easy to implement, and the application is suitable for large-scale industrial application. The carbon, nitrogen and phosphorus compositions of the extract meet the requirements of directional acid production or other conversion and utilization modes, and the remaining algal cell precipitate is rich in nitrogen and phosphorus, and can be used for further extraction of nucleic acids, proteins, polypeptides or amino acids, and can also be directly used as a fertilizer.

The present invention provides a short peptide, a polypeptide extract of Chlorella pyrenoidosa and its preparation method and application, belonging to the technical field of functional polypeptides. The present invention can overcome the problem that the performance of microalgae polypeptide lipid-lowering products in the prior art needs to be improved. The short peptide FLGPF provided by the present invention has a small molecular weight and a high inhibitory rate on the activity of pancreatic lipase. The content of the short peptide FLGPF in the polypeptide extract of Chlorella pyrenoidosa obtained by the preparation method of the present invention can reach 7.1-8.6%, and has good application prospects. The polypeptide extract of Chlorella pyrenoidosa provided by the present invention can significantly reduce the contents of triglyceride and cholesterol in Caenorhabditis elegans, indicating that it has good application prospects in the development of weight loss functional foods or health products. The present invention provides technical support for the development of safe and effective pancreatic lipase inhibitors, and also has important significance for promoting the development of the Chlorella industry.

The application provides application of Chlorella algae strains in Macrobrachium rosenbergii breeding and virus inhibition, which is that the Chlorella algae strains are expanded in an optimized biogas liquid culture system, and the algal body or algal liquid is collected and put into the water body of the Macrobrachium rosenbergii breeding; the Chlorella algae strains are selected from Chlorella pyrenoidosa Chlorella pyrenoidosa , or Chlorella sorokiniana Chlorella sorokiniana . The application finds that if disease prevention and control is the core, Chlorella pyrenoidosa is preferred; if promoting growth and improving yield is the core, Chlorella sorokiniana is preferred; and Chlorella pyrenoidosa and Chlorella sorokiniana have significant and broad-spectrum direct antiviral activity.

The invention belongs to the technical field of veterinary biological products, and discloses chlorella pyrenoidosa CV-5, a probiotic microcapsule and application of the probiotic microcapsule in preparation of products for improving intestinal health of calves. The preservation number of the chlorella pyrenoidosa CV-5 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.15206, and the Latin name of the chlorella pyrenoidosa CV-5 provided by the invention is Chlorella pyrenoidosa. Compared with other algae, the chlorella pyrenoidosa CV-5 provided by the invention has remarkable advantages in the aspect of improving the intestinal health of calves. On the basis, chlorella pyrenoidosa CV-5 powder and pectin are matched with glucose oxidase to serve as wall materials, dominant probiotic strains, sodium butyrate, trypsin and specific prebiotics serve as core materials, and the feed capable of remarkably improving the survival rate of the probiotics in the calf wrinkled stomach environment, improving the survival rate of the probiotics in the calf wrinkled stomach environment and improving the survival rate of the probiotics in the calf wrinkled stomach environment is further developed. Meanwhile, the abundance of pathogenic bacteria in intestinal tracts of the diarrhea calves is reduced. The feed additive is suitable for the livestock breeding industry, and especially has a good application prospect in the field of improving the intestinal health of the calves.