19 results about "Pyrenoid" patented technology

The invention provides a method for regulating and controlling growth and metabolism of caproic acid bacteria by using marine active peptides in a baijiu fermentation process. According to the method, the marine active peptides are added in an anaerobic fermentation stage of baijiu preparation to regulate and control the growth and metabolism of the caproic acid bacteria; wherein the marine active peptide is chlorella pyrenoidosa peptide, schizochytrium limacinum peptide, fish albumin peptide or euphausia superba peptide. According to the method, active peptides with specific sources are added into an anaerobic culture system, so that the growth of caproic acid bacteria can be promoted, energy metabolism can be enhanced, and the activity of key enzymes such as caproic acid dehydrogenase and caproic acid synthase can be activated, and therefore, the generation amount of flavor acids such as caproic acid and butyric acid can be increased, the fermentation quality can be improved, and the flavor level and esterification precursor substance content of Baijiu can be improved.

This invention belongs to the field of functional food processing technology, specifically relating to a method for improving the flavor of a chlorophyll-deficient Chlorella proteoglycan mutant. The method includes the following steps: S1, obtaining a Chlorella proteoglycan mutant strain; S2, centrifuging the Chlorella proteoglycan mutant strain and collecting the algal sludge, then pulverizing the collected algal sludge and dispersing it in water to obtain an algal powder suspension, then adding a compound enzyme to the algal powder suspension, and inactivating the enzyme after the reaction; S3, cooling the system to room temperature, adding glucose and stirring to dissolve, sterilizing, inoculating with a compound bacterial strain and fermenting, and sterilizing again after fermentation; S4, cooling the fermented system to room temperature, then adding β-cyclodextrin and stirring, and drying the system after stirring to obtain the algal protein product. This invention employs a synergistic process of compound enzymatic hydrolysis, compound bacterial fermentation, and β-cyclodextrin encapsulation to remove the off-flavor of the mutant algae from the source, significantly improving the flavor and enhancing the product's storage stability.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

The present application relates to a kind of illumination culture methods for improving chlorella pyrenoidosa biomass, the illumination culture method includes the following steps: chlorella pyrenoidosa stock solution is inoculated in sterile liquid medium, and chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light.The present application develops a kind of culture method for chlorella pyrenoidosa, i.e.chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light, the culture method is simple to operate, easy to implement, the method can significantly improve the growth rate and biomass accumulation of chlorella pyrenoidosa, realize the high-quality large-scale production of chlorella pyrenoidosa;It can also reduce the production energy consumption per unit biomass, solves the technical problem that yield increase and consumption reduction are difficult to consider in microalgae culture.

The invention discloses a method for screening a chlorella pyrenoidosa mutant, which comprises the following steps: carrying out activation, culture and mutagenesis on chlorella pyrenoidosa to obtain a chlorella pyrenoidosa solution; wrapping the mutagenized algae liquid in micro-droplets, and collecting the micro-droplets in a conduit; culturing the wrapped micro-droplets under a given condition; and detecting a fluorescence signal of the cultured micro-droplet, and screening out chlorella pyrenoidosa with fast and slow growth rate based on the intensity of the fluorescence signal. The method has the beneficial effects of high flux, high precision and the like, and overcomes the defects of low flux and high false positive of the traditional method.

The invention relates to the technical field of food, in particular to microalgae emulsion and a preparation method thereof. The microalgae emulsion provided by the invention comprises chlorella pyrenoidosa subjected to green removal; and a liquid phase for dispersing the chlorella pyrenoidosa, wherein the liquid phase consists of water and corn oil. The chlorophyll content of the chlorella pyrenoidosa subjected to green removal is extremely low, meanwhile, the protein content of the chlorella pyrenoidosa subjected to green removal is extremely high, and the formed microalgae emulsion can be milky white, so that the adverse effect of conventional green chlorella on food sense organs can be reduced, and meanwhile, the green chlorella pyrenoidosa subjected to green removal is prepared based on the effect of a liquid phase composed of water and corn oil and the chlorella pyrenoidosa subjected to green removal. The microalgae emulsion disclosed by the invention also has good stability; therefore, the microalgae emulsion disclosed by the invention has very good stability and consumer acceptability, and has a very good application prospect in the field of foods.

The invention discloses a method for producing high-organic-calcium microalgae single-cell protein by recycling white spirit vinasse, which comprises the following steps: by taking the white spirit vinasse as a nutrient medium and chlorella pyrenoidosa as a single-cell protein source, firstly, pretreating the white spirit vinasse and carrying out microbial acid-producing fermentation, and dissolving the organic acid generated by fermentation into low-cost inorganic calcium (calcium carbonate) in a system to obtain the high-organic-calcium microalgae single-cell protein. When the pH of the system is adjusted and the acid inhibition in the fermentation process is relieved, an available calcium source and an organic acid carbon source are provided for the chlorella pyrenoidosa; then carrying out directional domestication breeding on the chlorella pyrenoidosa to improve the tolerance, the growth efficiency and the calcium enrichment rate of the chlorella pyrenoidosa; and finally, culturing the domesticated chlorella pyrenoidosa by using a vinasse culture medium subjected to fermentation conversion to produce microalgae single-cell protein rich in organic calcium. According to the invention, the resource reutilization of food wastes is realized, the microalgae culture cost is reduced, the organic calcium content in the obtained single-cell protein product is increased (greater than or equal to 0.48%), and the single-cell protein product can be used for preparing functional foods.

The invention discloses a composition containing agilawood extract and a preparation method and application thereof, and belongs to the technical field of biology, the composition comprises 5-20 parts of agilawood extract, 20-60 parts of functional peptide and 5-25 parts of seaweed functional component; wherein the seaweed functional component is used for forming an embedding, gel or slow-release structure; the functional peptide is preferably chlorella pyrenoidosa peptide and is prepared by combining freeze-thaw ultrasonic-assisted enzymolysis with cation exchange chromatography; the agilawood extract is preferably an agilawood chromone enriched extract; during preparation, the seaweed functional component is dissolved in water, the agilawood extract is added for dispersion or emulsification, then the functional peptide is added for uniform mixing, and freeze-drying, spray-drying or forming treatment is performed to obtain the seaweed functional peptide. The composition can effectively improve the stability and bioavailability of active ingredients, and has a good application prospect in preparation of products for auxiliary protection of acute, alcoholic, drug-induced or fatty liver injuries.

The application discloses a method for extracting and separating high-carbon and low-nitrogen and phosphorus organic components in blue algae. The method adopts a process of step-by-step extraction by using an alkaline solution, and when necessary, a mild wall breaking is assisted by adding a low-concentration oxidizing agent, and the interaction between biological molecules is weakened by using sodium chloride, so that high-carbon organic components such as polysaccharides and lipids are effectively decomposed and extracted, and the outflow of high-nitrogen and high-phosphorus components such as proteins and nucleic acids is largely prevented, and the high-carbon and low-nitrogen and phosphorus organic components in the blue algae are successfully obtained. The reagents used in the application are cheap, safe and environmentally friendly, the operation condition is mild, the energy consumption is low, the application is easy to implement, and the application is suitable for large-scale industrial application. The carbon, nitrogen and phosphorus compositions of the extract meet the requirements of directional acid production or other conversion and utilization modes, and the remaining algal cell precipitate is rich in nitrogen and phosphorus, and can be used for further extraction of nucleic acids, proteins, polypeptides or amino acids, and can also be directly used as a fertilizer.

The application provides application of Chlorella algae strains in Macrobrachium rosenbergii breeding and virus inhibition, which is that the Chlorella algae strains are expanded in an optimized biogas liquid culture system, and the algal body or algal liquid is collected and put into the water body of the Macrobrachium rosenbergii breeding; the Chlorella algae strains are selected from Chlorella pyrenoidosa Chlorella pyrenoidosa , or Chlorella sorokiniana Chlorella sorokiniana . The application finds that if disease prevention and control is the core, Chlorella pyrenoidosa is preferred; if promoting growth and improving yield is the core, Chlorella sorokiniana is preferred; and Chlorella pyrenoidosa and Chlorella sorokiniana have significant and broad-spectrum direct antiviral activity.

The invention belongs to the technical field of veterinary biological products, and discloses chlorella pyrenoidosa CV-5, a probiotic microcapsule and application of the probiotic microcapsule in preparation of products for improving intestinal health of calves. The preservation number of the chlorella pyrenoidosa CV-5 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.15206, and the Latin name of the chlorella pyrenoidosa CV-5 provided by the invention is Chlorella pyrenoidosa. Compared with other algae, the chlorella pyrenoidosa CV-5 provided by the invention has remarkable advantages in the aspect of improving the intestinal health of calves. On the basis, chlorella pyrenoidosa CV-5 powder and pectin are matched with glucose oxidase to serve as wall materials, dominant probiotic strains, sodium butyrate, trypsin and specific prebiotics serve as core materials, and the feed capable of remarkably improving the survival rate of the probiotics in the calf wrinkled stomach environment, improving the survival rate of the probiotics in the calf wrinkled stomach environment and improving the survival rate of the probiotics in the calf wrinkled stomach environment is further developed. Meanwhile, the abundance of pathogenic bacteria in intestinal tracts of the diarrhea calves is reduced. The feed additive is suitable for the livestock breeding industry, and especially has a good application prospect in the field of improving the intestinal health of the calves.

The application discloses a Lactiplantibacillus plantarum and application of the Lactiplantibacillus plantarum in fermentation beverage of Chlorella pyrenoidosa. In the application, a Lactiplantibacillus plantarum is screened and separated from an outdoor Chlorella pyrenoidosa cultivation pond, and is named as Lactiplantibacillus plantarum HN3. The Lactiplantibacillus plantarum HN3 has been preserved in the Guangdong Microbial Culture Collection Center, No. 59 Building, 5th Floor, Guangzhou City, on July 26, 2024, and has a preservation number of GDMCC No: 64816. The Lactiplantibacillus plantarum HN3 has good fermentation performance, acid tolerance and bile salt tolerance, can degrade bad odor substances of Chlorella pyrenoidosa liquid, produce new pleasant odor substances, and can be used as a production strain of Chlorella pyrenoidosa fermentation beverage.

The invention belongs to the technical field of chlorella deep processing, and particularly relates to a chlorella pyrenoidosa precise active peptide capable of promoting GLP1 secretion, reducing blood sugar and lipid and enhancing immunity, and a preparation method and application thereof. The preparation method comprises the following steps: firstly, mixing chlorella pyrenoidosa powder with water, and pretreating at high temperature; after pretreatment, cellulase, pectinase and alkaline protease 1 are sequentially added for hydrolysis, and then the chlorella pyrenoidosa peptide is obtained through enzyme deactivation, centrifugal filtration, drying and powder spraying. The chlorella pyrenoidosa peptide prepared by the method disclosed by the invention has the effects of resisting oxidation, inhibiting DPP-IV activity, promoting GLP-1 secretion, enhancing immunity and the like. The preparation method of the chlorella precise active peptide provided by the invention has the advantages of low equipment requirement, simplicity and convenience in operation, easiness in industrial amplification and the like, and a feasible technical scheme is provided for large-scale production of functional active peptides.

The application provides a three-dimensional multi-stage hole phosphonated chitosan / microalgae-based aerogel and a preparation method and application thereof, and belongs to the technical field of aerogel preparation. The three-dimensional multi-stage hole phosphonated chitosan / microalgae-based aerogel (aCSP / CP) is prepared by taking chitosan (CS), phosphonated chitosan (CSP) and chlorella pyrenoidosa powder (CP) as raw materials. The selectivity of CP to U(VI) is low, but the total ion adsorption capacity is higher, and more coexisting ions can be adsorbed. The selectivity of CSP to U(VI) is greatly improved. The high affinity and high selectivity between the phosphorus functional groups in CSP and U(VI) can improve the selective adsorption of U(VI). In the application, CP and CSP synergistically act together to realize the high selective adsorption of U(VI).

The application provides a preparation method of Chlorella pyrenoidosa protein peptide-calcium chelate, and the preparation method comprises the following steps: S1, Chlorella pyrenoidosa protein is dissolved in deionized water, the pH is adjusted to 2-13, stirring is carried out for 1-5 hours, centrifugation is carried out, the supernatant is taken, and a Chlorella pyrenoidosa protein treatment solution is obtained; S2, calcium chloride is added to the Chlorella pyrenoidosa protein treatment solution, water bath chelation is carried out at 35-38 DEG C for 1.8-2.2 hours, centrifugation is carried out, anhydrous ethanol is added to the supernatant, standing is carried out, centrifugation is carried out again, the precipitate is collected, and freeze-drying is carried out, and the Chlorella pyrenoidosa protein peptide-calcium chelate is obtained. The preparation process is simple, no toxic side reagent is used, energy consumption is low, the prepared product is verified to have a calcium absorption promoting effect through animal experiments, and solid theoretical support is provided for the development and application of the Chlorella pyrenoidosa protein peptide-calcium chelate as a new bone health promoter.

The invention relates to the technical field of microbial culture technology and food processing science, in particular to an efficient and rapid culture method for chlorella pyrenoidosa rice, which comprises the following steps: step 1, gelatinizing a rice matrix part; step 2, carrying out charge reversal activation on the algae species: carrying out low-shear propagation in a culture medium containing rice starch hydrolysate to an inflection point in a logarithmic phase; adding cationic polysaccharide until the Zeta potential on the surface of the algae cell reaches a charge zero point; step 3, performing negative pressure permeation inoculation; 4, light-gas coupling culture: red light and blue light are alternately irradiated, bottom airflow associated with the thickness of the rice bed is started in the red light period, and humidity control and humidification are started in the blue light period; the culture temperature is dynamically increased based on chlorophyll fluorescence intensity change; and step 5, enzyme-magnetic synergistic harvesting. According to the method, the field planting efficiency of the chlorella pyrenoidosa in the rice matrix is effectively improved, and the growth cycle of algae is accelerated.

The invention relates to the technical field of microalgae biology, in particular to a high-biomass and high-photosynthetic-efficiency chlorella pyrenoidosa mutant strain J-1 which is obtained by mutagenizing wild type chlorella pyrenoidosa through an atmospheric pressure room temperature plasma (ARTP) mutagenesis technology and combining an efficient screening strategy. When the strain is cultured for 4 days under a standard autotrophic condition, the biomass reaches 1.23 mg / mL, the OD750 reaches 6.27, and when the strain is cultured for 5 days under a high light stress condition, the OD750 reaches 16.70; according to the invention, the growth performance is obviously enhanced: the biomass of J-1 is higher than that of WT under autotrophic, mixotrophic or heterotrophic conditions, and the biomass of a J-1 mutant strain reaches 1.23 mg / mL and is increased by 53.8% compared with that of a wild type (0.80 mg / mL) after the J-1 mutant strain is cultured for 4 days under a standard autotrophic condition (50 [mu] mol * m <-2 > * s <-1 >); the OD750 reaches 6.27 and is higher than 5.75 of a wild type, the OD750 reaches 2.18 mg / mL (WT is 1.75 mg / mL) on the fourth day of mixotrophic culture, the OD750 reaches 1.39 mg / mL (WT is 1.05 mg / mL) on the fourth day of heterotrophic culture, and it is indicated that biomass can be efficiently accumulated in different nutritional modes.

The application discloses a microbial remediation agent for heavy metal remediation and a preparation method and application thereof, and is prepared by loading pseudomonas aeruginosa on a carrier; the carrier comprises the following components in parts by weight: 8-22 parts of chlorella vulgaris algae liquid, 1-3 parts of silicon fertilizer, and 1-3 parts of nano zero-valent iron; the silicon fertilizer is obtained by calcining a mixture of granite stone powder, marble powder and alkali activator at a mass ratio of 8-12:0-12:0.5-4; and the alkali activator is solid sodium hydroxide or potassium hydroxide. The microbial remediation agent has high activity, fast reproduction, is environment-friendly and pollution-free, and is low in cost; can change the form of heavy metals, converts the heavy metals from a high-toxicity form into a low-toxicity form, reduces the toxicity and migration of the heavy metals, and significantly reduces the pollution caused by the heavy metals.

The invention provides chlorella pyrenoidosa, phycomycete symbiotic suspension, phycomycete symbiotic microspheres and application of the chlorella pyrenoidosa, the phycomycete symbiotic suspension and the phycomycete symbiotic microspheres in preparation of a vibrio parahaemolyticus The invention provides application of chlorella pyrenoidosa in preparation of a vibrio parahaemolyticus antagonist. The chlorella pyrenoidosa can inhibit proliferation of vibrio parahaemolyticus in a water body. The invention provides a phycomycete symbiotic suspension. The phycomycete symbiotic suspension comprises the chlorella pyrenoidosa and a bacillus subtilis strain JSHY-K3. According to the invention, the phycomycetes are compounded to form a symbiotic form of phycomycetes combination, and compared with a single strain JSHY-K3 and single chlorella pyrenoidosa, the antagonistic effect on vibrio parahaemolyticus after compounding is obviously enhanced, and the purification effect on water is obviously enhanced. The invention further provides the phycomycete symbiotic microspheres, the phycomycete symbiotic microspheres are green, sustainable and low in cost, and the problems of aquaculture water pollution and water resource waste can be practically solved.