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Preparation method for oleuropein component in olea europaea leaves

A technology of olive leaves and glycosides, which is applied in the field of preparation of glycosides in olive leaves, can solve the problems of resource waste and underutilization, and achieve the effect of easy operation

Inactive Publication Date: 2020-06-02
启东茂材实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of olives is mainly to produce olive oil, but the large amount of olive leaves produced by pruning each year has not been fully utilized, resulting in a huge waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for preparing glycosides in olive leaves, comprising the steps of: taking 30 parts of dried olive leaves, adding 10 times the amount of ethanol with a volume fraction of 65%, heating and refluxing at 70°C for 3 times, each time for 2.5 hours, After the extracts are combined, the solvent is recovered by rotary evaporation, and the residue is dissolved in water to obtain the extract after it is nearly dry; the AB-8 resin is put into a 40cm×2cm glass column, and the height of the resin is 20cm; the extract is adsorbed on the column, and the volume flow 5mL / min, wash the resin column with 3 times the column volume of distilled water after adsorption, then elute with 65% ethanol with a volume fraction of 6 times the column volume, the volume flow rate is 2mL / min, collect the 65% ethanol eluate, and recover under reduced pressure Solvent, and then separated by silica gel column chromatography, eluted with chloroform-methanol with a volume ratio of 48:1, 35:1, 24:1, 13...

Embodiment 2

[0016] A method for preparing glycosides in olive leaves, comprising the steps of: taking 25 parts of dried olive leaves, adding 10 times the amount of ethanol with a volume fraction of 60%, heating and refluxing at 65°C for 2 times, each time for 2 hours, extracting After the liquid is combined, the solvent is recovered by rotary evaporation, and the residue is dissolved in water to obtain the extract after it is nearly dry; the AB-8 resin is put into a 40cm×2cm glass column, and the resin height is 20cm; the extract is adsorbed on the column, and the volume flow rate is 5mL After adsorption, wash the resin column with 3 times the column volume of distilled water, then elute with 65% ethanol with a volume fraction of 6 times the column volume, the volume flow rate is 2mL / min, collect the 65% ethanol eluate, and recover the solvent under reduced pressure , and then separated by silica gel column chromatography, eluted with chloroform-methanol at a volume ratio of 48:1, 35:1, 24...

Embodiment 3

[0018] A method for preparing glycosides in olive leaves, comprising the following steps: take 35 parts of dried olive leaves, add 10 times the amount of ethanol with a volume fraction of 70%, heat and reflux at 75°C for 4 times, each time for 3 hours, extract After the liquid is combined, the solvent is recovered by rotary evaporation, and the residue is dissolved in water to obtain the extract after it is nearly dry; the AB-8 resin is put into a 40cm×2cm glass column, and the resin height is 20cm; the extract is adsorbed on the column, and the volume flow rate is 5mL After adsorption, wash the resin column with 3 times the column volume of distilled water, then elute with 65% ethanol with a volume fraction of 6 times the column volume, the volume flow rate is 2mL / min, collect the 65% ethanol eluate, and recover the solvent under reduced pressure , and then separated by silica gel column chromatography, eluted with chloroform-methanol at a volume ratio of 48:1, 35:1, 24:1, 13:...

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PUM

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Abstract

The invention discloses a preparation method for an oleuropein component in olea europaea leaves. The method comprises the following steps: taking the dried olea europaea leaves, adding ethanol with avolume fraction of 60%-70%, performing heating reflux extraction for 2-4 times at 65-75 DEG C, wherein reflux extraction is performed for 2-3 h each time, combing the extracted liquids, performing rotary evaporation to recover a solvent, and after the liquid is nearly dried, dissolving the residues in water to obtain an extract liquid; putting AB-8 resin in a glass column with a resin height of 20 cm; and performing adsorption on the extract liquid through the column, after the adsorption is completed, washing the resin column by using distilled water with a volume of 3 times of a column volume, performing elution by using ethanol with a volume fraction of 65% and a volume of 6 times of the column volume, collecting a 65% ethanol eluent, recovering the solvent under reduced pressure, performing silica gel column chromatography separation, performing elution by using chloroform-methanol, collecting a 13:1 eluted fraction, and performing concentration to obtain the oleuropein component.The method is simple, fast, and easy to operate, and can comprehensively and effectively obtain the oleuropein component in the olea europaea leaves, and the oleuropein component has antibacterial, anti-inflammatory and anti-oxidation effects, and can be used for preparation of medicines.

Description

technical field [0001] The invention relates to a preparation method of glycoside components in olive leaves. Background technique [0002] olive Olea europaea L. It is an evergreen tree of Oleaceae Oleaceae, mainly distributed in Mediterranean countries, and widely planted in Gansu, Sichuan, Yunnan and other places in China. It is an important economic forest crop. At present, the application of olives is mainly to produce olive oil, and the large amount of olive leaves produced by pruning each year have not been fully utilized, resulting in a huge waste of resources. Modern phytochemical studies have found that olive leaves are rich in compounds such as secoiridoids, flavonoids and sterols. Modern pharmacological studies have proved that the split iridoid compound-oleuropein has various pharmacological activities such as antibacterial, anti-inflammatory, anti-oxidation, anti-diabetic, and hypolipidemic. Olive leaves contain a large number of oleuropein compounds with ...

Claims

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Application Information

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IPC IPC(8): A61K36/32A61P31/04A61P31/10A61P29/00A61P39/06A61K127/00
CPCA61K36/32A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P29/00A61P31/04A61P31/10A61P39/06
Inventor 不公告发明人
Owner 启东茂材实业有限公司
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