The present invention discloses an analytical method for simultaneously and rapidly detecting phenolic and
triterpenoid compounds in olive
processing byproducts. The
rapid detection method of the present invention comprises the following two steps: 1)
raw material pretreatment: using olive
processing byproducts (leaves, branches,
pomace, etc.) as raw materials, naturally air-dried to a
moisture content of no more than 5% away from
sunlight, crushed to about 0.42 mm, adding an appropriate volume of
ethanol-water of a certain concentration as an extraction
solvent for simultaneously enriching phenolic and triterpenoids, extracting for a period of time under the assistance of
ultrasound, filtering while hot, and passing through a 0.45 μm organic filter membrane after constant volume with an
alcohol-water
solvent, and the obtained filtrate is used as a subsequent test. 2) Liquid
chromatography-
mass spectrometry (HPLC-DAD-MS) detection: Under appropriate chromatographic and other detection conditions, it is possible to simultaneously detect a variety of phenolic and
triterpenoid compounds in olive
processing byproducts. Specifically, Agilent's Poroshell 120, EC-C18 (150mm×3.0mm id, 2.7μm particle size)
chromatographic column was used, mobile phase A was
acetonitrile and mobile phase B was
distilled water containing acidic components, and binary
gradient elution was performed. The
elution gradient program was as follows:
acetonitrile (
solvent A) started from 5% at 0.1min, changed from 5% to 40% at 40min, maintained at 40% to 45min, then increased to 70% at 50min, maintained at 70% to 60min; increased to 100% at 65min, maintained at 100% until 68min, and finally returned to 5% at 70min. The detection wavelengths were set to 210, 240, 280 and 350nm, and phenolic and
triterpenoid compounds in the sample solution could be successfully detected at a certain
column temperature and a certain flow rate. The detection method of the present invention has the characteristics of simplicity, easy operation, high efficiency and wide applicability, and can also be used to simultaneously detect phenolic and triterpenoid compounds in processing by-products of olives of different varieties.