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160results about How to "Shorten rooting time" patented technology

Tissue culture rapid propagation method for Japanese red maple

The invention relates to a tissue culture rapid propagation method for Japanese red maple which obtains a large quantity of young plants by six steps of the selection and sterilization of explants, the best induction culture medium and growth medium, the preparation of aseptic seedling, the proliferation of sprouts, proliferation growth, rootage culture and hardening-seedling and transplantation. The tissue culture rapid propagation method has the beneficial effects that an asexual propagation technique is utilized for improving the traditional mode of the young plant cultivation. In the human-made optimized environment, the physical optimization difficult problem of the young plants is solved; and various good seed characteristics in plants such as cold-resistant, anti-drought, disease-resistant, insect-resistance, rapid growth and the like are transplanted and optically combined to create new-style fine varieties; the rootage rate of tissue cultured seedlings reaches more than 95%; and the transplantation survival rate reaches more than 85%; the rootage time is greatly shortened, which is directly related to the childness or young nature of the plants which is aroused. The method has the great significant for accelerating the expanding propagation of the new species and reducing the cost of the seed seedling.
Owner:CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY

Shoot cutting seedling-culturing method of cyclocarya paliurus

A shoot cutting seedling-culturing method of cyclocarya paliurus mainly comprises the steps of processing of a shoot mother tree, preparation and processing of a cutting medium, processing of auxin, trimming and cutting of a shoot, management after cutting, transplantation and the like. The new seedling-culturing method through the new medium and a new hormone recipe resolves the problems that cutting callus tissue of cyclocarya paliurus is difficult to form, organ differentiation is difficult, root growth time is long and root growth rate is low, is a method for developing and utilizing the plant resources of cyclocarya paliurus protectively, and provides powerful technical guarantee for reutilization of germplasm resources.
Owner:HUNAN ACAD OF FORESTRY

Rooting method for tissue culture of Chinese fir

The invention provides a fir tissue culture rooting method. Small fir multiplication subculture seedlings are cut and transplanted to a fir rooting culture medium for culturing and rooting tissue, and the fir rooting culture medium comprises an improved MS culture medium, 0.4-0.8mg / L of ABT1#, 0.1-0.5mg / L of IBA and 2-6mL / L of root sun diluent. After the fir rooting culture medium is adopted, the rooting time of firs is shortened to 16 to 22 days; after 22 days of culture, the highest rooting rate achieves 96 percent, and nursery stocks grow rapidly so as to decrease the culture cost of the seedlings and realize the industrialized production of the firs.
Owner:厦门涌泉科技有限公司

Acer rubrum cuttage and seedling culturing method

The invention discloses an acer rubrum cuttage and seedling culturing method. The acer rubrum cuttage and seedling culturing method comprises the following steps: (1) preparing a cuttage medium; (2) selecting cutting slips; (3) treating before cutting; (4) transplanting the cutting slips; (5) managing after cutting. The cuttage medium prepared by the invention has a plurality of good physiochemical properties including better air permeability, porosity, moisture and fertilizer retaining capabilities and the like; a lower-layer medium for growth and an upper-layer medium with heat-insulation, moisture-keeping and antibacterial performances, which are separately arranged, meet a growth principle of cuttage strips, so that the survival rate is improved; before cuttage, the cutting slips are immersed for two times so that the growth speed of roots is greatly improved and the rooting time is shortened; the cutting slips start to take roots 6-9 days after the cuttage and all the cutting slips can take roots after 25 days; the average root quantity is more than 12 and the survival rate of the cutting slips is also up to more than 93%; seedlings have developed root systems, are ordered and strong and have stable properties, so that the economic values are higher.
Owner:阜南县田隆花木种植有限公司

Single node and side shoot cutting seedling raising method adopting plug tray and applied to tea trees

A single node and side shoot cutting seedling raising method adopting a plug tray and applied to tea trees comprises the following steps: greenhouse preparation, substrate preparation, loading of the substrate to the plug tray, cutting selection, shoot cutting, processing of cutting shoots with rooting powder, cutting and seedling management. Moisture of the substrate and moisture of the cutting shoots are kept in an intermittent spraying manner, the survival rate of the cutting shoots is increased, and the callus forming time and rooting time are shortened; seedlings develop fast after rooting of the cutting shoots provided with the side shoots, and the seedling raising period is shortened; seedling raising through the light substrate and the plug tray is adopted, the root systems of the tea tree seedlings are in a net shape and cluster with the substrate, so that the seedlings are not prone to damage during pulling up, transportation and transplanting, the transplanting survival rate is increased, and the problems of long seedling raising period of single node cutting in an open field by adopting a double-layer film, difficult moisture management of single node and side shoot cutting and the like are solved.
Owner:INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI

Cuttage method of rose

The invention discloses a cuttage method of rose. The method includes the steps of first, selecting and making cuttings; second, preparing nutrient solution; third, making cutting blocks; fourth, performing cuttage, namely inserting made rose stems into flower mud, pouring the cuttage nutrient solution into a plastic tray, placing the rose stems, inserted into the flower mud, in the plastic tray, ensuring one third of each cutting block is immerged in the nutrient solution, placing the plastic tray in the environment with 2000-3000lux illumination and good ventilation, and moving the plastic tray with the flower mud into a flowerpot after all cuttings root. The method has the advantages that required materials are convenient to obtain, and no high technical demands are require; unlike all-light spraying requiring many automatic devices, the method requires regular addition of the nutrient solution only; rooting is fast; antibiotics are continuously used in the cuttage process, invasion of pathogens into cuttings can be controlled effectively, cutting quality is improved, and fewer cuttings degrade; transplanting survival rate is high.
Owner:HUOCHENG COUNTY KEFA TECH RISK INVESTMENT

Hydrangea cuttage reproduction method

The invention provides a hydrangea cuttage reproduction method which includes the steps of firstly, cuttage matrix selection; secondly, scion selection; thirdly, shearing; fourthly, cuttage. Compared with the prior art, the hydrangea cuttage reproduction method has the advantages of shortening hydrangea cultivation time of the prior art, more importantly, by the technology that two buds are distributed on each section, rooting time can be shortened evidently, rooting number and rate are increased greatly, reproduction yield is increased, and rooting agent use is reduced.
Owner:JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY

Blueberry twig plant cuttage root-taking cultivation method

The invention relates to a blueberry twig plant cuttage root-taking cultivation method. The specific cultivation operation comprises the steps of: (1) preparation of a root-taking liquid, to be specific, respectively adding a lanthanum sulfate solution and an indolebutyric acid solution in bacteria free water and mixing uniformly to prepare the root-taking liquid; (2) selecting strong blueberry twigs of 20-25 cm in length, removing leaves at the bottom ends of the blueberry twigs and soaking the bottom ends of the blueberry twigs in the root-taking liquid for 5-10 min; (3) vertically inserting the bottom parts of the blueberry twigs into a root-taking substrate, wherein the root-taking substrate is formed by uniformly mixing turf and fine pearlite according to a volume ratio of 1: 1; and cultivating the blueberry twigs at cultivation conditions including 20-30 DEG C of temperature, 80%-90% of relative humidity and 40%-60% of shading rate for 30 days to obtain new blueberry seedlings. The root-taking time of the blueberry twigs is shortened from 90 days to 30 days, the root-taking rate of the blueberry twigs is more than 80% and the survival rate of the new blueberry seedlings is more than 90%.
Owner:宣城明美蓝莓科技有限公司

Method for reducing aerial root of tissue culture seedling of hydrangea

The invention belongs to the technical field of plant tissue breeding, and in particular to a method for reducing the aerial root of hydrangea, aiming at solving the problem that the aerial root is generated in the tissue culture process of the hydrangea and improving the survival rate of tissue culture seedling of the hydrangea. The method is realized by the following steps: the tissue culture seedling of the hydrangea is taken as an experimental material and is inoculated in a liquid rooting medium (1 / 2B5+NAA or IBA 0.1-1.0mg / L+sugar 10g / L pH 4-7) of perlite (40-60ml)+25-35ml; after 30 days, no aerial root is generated in the tissue culture seedling, the rooting rate in a culture flask reaches 100%, and the transplanting survival rate reaches 100%. The method not only improves the transplant survival rate and simplifies the operating procedures of transplantation, but also saves the cost of tissue culture by using the perlite to replace agar and recycling the perlite.
Owner:HUAZHONG AGRICULTURAL UNIVERSITY

Northeast osier branch cuttage method

The invention belongs to methods for cultivating and expanding of bush willows and particularly relates to a northeast osier branch cuttage method. The northeast osier branch cuttage method aims to solve the problems that by the adoption of an existing northeast osier cultivating and expanding technology, the anti-bacterial capacity is low, and the rate of survival of branches is low. The northeast osier branch cuttage method comprises the steps that northeast osier branches are selected, soaked and then are inserted into a sand culture matrix, the branches are irrigated with a prepared and improved Hoagland nutrient solution and the like, the braches are cultivated in a greenhouse for two mouths, the well-developed branches are transplanted to an outdoor environment with sufficient illumination and moisture on the premise that roots are not damaged, and the braches are planted according to the line space of 1-2 m * 1-2 m. By the adoption of the northeast osier branch cuttage method, the rooting time of the northeast osier can be shortened, the cultivation survival rate is increased, the germinating capacity is high, and the rate of survival of branches reaches over 87%; in addition, due to the fact that the sand culture matrix can be utilized repeatedly, materials are saved, cost is reduced, and environmental friendliness is achieved.
Owner:NORTHEAST NORMAL UNIVERSITY

Open rooting method for sugarcane tissue culture seedlings

The invention discloses an open rooting method for sugarcane tissue culture seedlings. According to the method, cespititious seedlings in a tissue culture differentiation stage of sugarcane are taken as materials; the cespititious seedlings are induced and rooted by a culture solution to grow in the open environment; the method is characterized by culturing in an open manner and inducing the sugarcane cespititious seedlings to root. The method is simple and liable to implement; the cost can be greatly saved; by virtue of the application of the open culture, the culture does not need to be completed in an artificial super clean bench and high-temperature sterilization culture solution is not required, so that the energy consumption is greatly reduced; meanwhile, by virtue of the open operation, the working efficiency is relative high; the labor is saved; the rooting inducing culture solution is simple in components and free of components such as saccharose and carrageenan, so that the cost is reduced. The open rooting method for sugarcane tissue culture seedlings is high in rooting rate and low in cost, and is applicable to large-scale production of the sugarcane tissue culture seedlings; the workload is greatly relieved; the production cost is reduced.
Owner:广州禾立田生物科技有限公司

Method for raising cyclocarya paliurus seedlings by cutting

The invention relates to a method for raising cyclocarya paliurus seedlings by cutting. The method comprises the following steps of using semi-lignified branches which grow in the same year and are cut from a parent tree of 3-5 years old as cuttings, and performing secondary cold storage on the cuttings so as to reserve nutrients and effective components; and arranging three layers of cutting mediums in seedling culturing plug trays, disinfecting the cutting mediums, enabling the disinfected cutting mediums to be exposed to the scorching sun, thoroughly watering the cutting mediums twice which are exposed to the scorching sun, then performing hormone treatment and burrowing treatment, inserting treated germinating branches into small holes, performing appropriate compaction, and controlling environment through a greenhouse. The method disclosed by the invention is high in resource utilization rate, simple and practical to operate, suitable for large-scale seedling raising by cutting, short in seedling culturing time, and high in rooting rate by cutting.
Owner:丁宗坚

Cutting propagation method of Michelia macclurei

InactiveCN103749138ASolve technical problems of clone seedling cultivationEasy to control growth potentialHorticulture methodsGenetic diversityMichelia macclurei
A cutting propagation method of Michelia macclurei includes the steps: stock plant planting and management, cutting medium sterilization, cutting collection, hormone treatment, and cuttage and post-cuttage management. The method is characterized in that high-quality seedlings are transplanted into medium-containing containers to cultivate cutting stock plants, trunks are cut to promote sprouting, cutting medium is sterilized, high-quality cuttings are treated and placed and cultivated in a sun shed, and high-quality cutting seedlings of Michelia macclurei are obtained. The method has the advantages that a cutting propagation method of Michelia macclurei is provided, cuttings are high in quality and availability, few pest and disease damages occur, survival rate is high, the seedlings can be directly taken out of a nursery for foresting, the effective and reliable method is provided for germplasm resource genetic diversity and germplasm resource conservation for Michelia macclurei, the requirements of China for precious tree species resources are met, and great economic benefit and social benefit are achieved.
Owner:GUANGXI FORESTRY RES INST

Method for raising seedlings of Prunus avium by cuttage

The invention relates to a method for raising seedlings of Prunus avium by cuttage, comprising the steps of (1) preparing a seedling raising substrate; (2) selecting and treating cuttings; (3) performing daily management. The method for raising seedlings of Prunus avium by cuttage provided herein is simple to perform; experiments show that by using the method of the invention, rooting rate of cutting seedlings of Prunus avium is greatly increased and is increased from conventional 62.5% to 97.5%, rooting is 24 days earlier than that in the conventional method, the effect is significant, and the method is easy to popularize and use.
Owner:HEFEI RUNYU AGRI TECH CO LTD

Soilless cutting seedling culture method of grapes

The invention mainly relates to the technical field of grape planting, and discloses a soilless cutting seedling culture method of grapes. The method includes the steps of clipping cutting slips, soaking the cutting slips, conducting cutting of the cutting slips, conducting post-cutting management, and exercising seedlings for cultivation. The method is simple and facilitates mass operation, the rooting time of the grape cutting slips is shortened to be 9-10 days, the rooting rate reaches 92%, the survival rate reaches 100%, the planting efficiency is obviously improved, and the economic income is increased by 9.6%; two-three-year-old grape shoots are clipped to serve as the cutting slips, many nutrients are accumulated in the cutting slips and can be supplied to callus formation of the cutting slips when external nutrients are not accumulated in the cutting slips yet, and the treating time and the rooting time are shortened.
Owner:JINZHAI XINTIANDI MODERN AGRI

Plant rooting agent and preparation method thereof

The invention provides a plant rooting agent and a preparation method thereof, and belongs to the technical field of agriculture. The plant rooting agent, in parts by weight, is prepared from the following raw materials: 1 to 4 parts of an ABT rooting agent, 6 to 12 parts of glycine, 5 to 10 parts of leucine, 5 to 8 parts of lysine, 3 to 5 parts of phenylalanine, 8 to 10 parts of proline, 5 to 8 parts of aspartic acid, 1 to 4 parts of tea acetic acid, 0.6 to 1.5 parts of indoleacetic acid, 30 to 45 parts of sucrose, 10 to 15 parts of ethyl alcohol, 1 to 3 parts of aspirin, 1 to 3 parts of lentinan, 0.5 to 1 part of B12, 3 to 5 parts of urea, 3 to 5 parts of potassium dihydrogen phosphate, 2 to 6 parts of zinc sulfate, 3 to 5 parts chitosan, 2 to 5 parts of folic acid and 150 to 200 parts of water. The rooting agent provided by the invention is capable of promoting the growing and the rooting of precious seedlings which are difficult to root and improving the quality of the seedlings.
Owner:JILIN LIZE BIOLOGICAL SCI & TECH CO LTD

Cutting propagation method of vernonia amygdalina

InactiveCN107125094AShorten rooting timeLow reproductive coefficientCultivating equipmentsGrowth plantComing out
The invention discloses a cutting propagation method of vernonia amygdalina. The method comprises the following steps: S1, selecting a seed bed; S2, preparing a plant growth regulator; S3, treating a cutting medium; S4, preparing cuttings; S5, carrying out cuttage; S6, carrying out tending management. After the steps are carried out, the cutting propagation method of the vernonia amygdalina, shown by the embodiment of the invention, enables cutting propagation survival rate to reach 100%, shortens the rooting time to 5-7 days, and greatly increases propagation coefficient; the method is simpler to operate and easy to implement, and reduces the cost; after the method is used, seedlings come out evenly and attractively, and a great deal of high-quality tidy nursery stocks can be provided for afforestation farmers within a short time.
Owner:湖南省森林植物园

Cuttage rapid propagation method for populus simonii

The embodiment of the invention discloses a cuttage rapid propagation method for populus simonii. The cuttage rapid propagation method comprises the following steps of S100, collecting and pretreatingcuttings; S200, conducting container-temperature control cuttage seedling growing on the cuttings, establishing a seedbed, conducting cuttage on the pretreated cuttings into nutrient bags, and separately controlling the temperature and humidity of the seedbed to designated ranges; S300, transplanting seedlings to a field and establishing a cutting orchard. According to the cuttage rapid propagation method, nutrient soil in the nutrient bags provides necessary nutrient substances for the growth of the cuttings, water storage sponge at the bottom of each nutrient bag makes root taking liquid permeate into the nutrient soil to promote root taking of plants, then a temperature control system and a sprinkling device regulate the temperature and humidity of the seedbed, in the early stage, thetemperature and humidity are increased to a condition adapting to the growth environment of beneficial microorganisms in the soil, and propagation of the beneficial microorganisms is accelerated; through products obtained through metabolism of the microorganisms, the growth of the cuttings is accelerated, the effective microbial community advantages are improved, the purpose of treating bacteria with microorganisms is achieved, the diseases of the cuttings are reduced, and the cuttage survival rate is increased.
Owner:INNER MONGOLIA AUTONOMOUS REGION ACAD OF FORESTRY SCI +1

Method for fast grape cutting root promoting

The invention discloses a method for fast grape cutting root promoting. The method comprises the steps of material preparation, arched shed soil preparation, hotbed manufacturing, grape cutting root promoting in a hotbed, irrigation, cuttage and the like. According to the method, grape cuttings are inserted into the hotbed, an electric heating wire is utilized to heat the hotbed, temperature needed by grape rooting is provided, a temperature controller is used for controlling the temperature in an arched shed, and enabling the temperature in the arched shed to be kept at 25 DEG C-28 DEG C, the grape cuttings can take roots fast at the suitable temperature, rooting time of the cuttings is shortened, and the survival rate is increased. The grape cuttings take roots at least 15-20 days earlier compared with that of traditional operation, the rooting rate can be improved by 20% or above, control is easy, and factory-like batch seedling cultivation can be achieved.
Owner:山东志昌农业科技发展股份有限公司

Cultivation method of millettia speciosa champ

ActiveCN107455259AThriveTall and robustGrowth substratesCulture mediaDiseaseBud
The invention belongs to the technical field of agricultural planting. A cultivation method of millettia speciosa champ comprises the following steps: a. collecting and treating seeds; b. carrying out tissue culture; c. sowing and breeding seedlings; d. transplanting; e. carrying out water and fertilizer management; f. pruning the seedlings. The cultivation method of the millettia speciosa champ takes the seeds as a material and realizes tissue culture speed propagation of the millettia speciosa champ in a way of bud propagation; the seedlings are obtained by the steps of primary culture, proliferative secondary culture, strong seedling cultivation and rooting culture; the obtained seedlings do not have the phenomenon of dehydration. The millettia speciosa champ cultivated by the method is good in growth and development, high in plant height and strong as well as developed in seedling root system, has thick and strong rattans, and is rapid in growth, more in tuberization, easy to manage and high in disease resistance.
Owner:台州中知英健机械自动化有限公司

Strawberry cuttage seedling-raising method

InactiveCN106561330AAvoid damageAvoid affecting the rooting and germination ratePlant cultivationCultivating equipmentsFragariaAbscisic acid
The invention belongs to the field of agricultural seedlings and particularly relates to a strawberry cuttage seedling-raising method. The method includes the steps that young seedlings to be subjected to cottage are immersed in a diluted abscisic acid ethanol solution, then placed in a vacuum freeze drier to be treated and then placed in an activating box for activating treatment, and then attapulgite, organic soil and Chinaberry seed extracts are mixed uniformly; after a sodium carboxymethylcellulose water solution is added and blended, the mixture serves as a wrapping material to wrap the outer layers of the young seedlings to be subjected to cottage; and then the seedlings are placed into a accelerating germination room for accelerating germination. The young seedlings to be subjected to cottage and treated through the method are high in germination rate and extremely low in disease occurrence rate after being transplanted in the field.
Owner:界首市艳兵家庭农场

Rooting agent for flower cuttage and preparation method thereof

The invention discloses a rooting agent for flower cuttage. The rooting agent for the flower cuttage is prepared from the following raw materials in parts by weight: 0.2 to 0.5 part of indoleacetic acid, 0.1 to 0.5 part of naphthaleneacetic acid, 20 to 50 parts of ethanol, 0.5 to 1 part of vitamin C, 1 to 5 parts of humic acid, 5 to 10 parts of tea seed meal, 10 to 20 parts of water, 1 to 3 parts of sodium hydroxide, 0.5 to 2 parts of chitosan, and 1 to 5 parts of plant extracting solutions. The plant extracting solutions are prepared from the following materials in parts by weight: 1 to 5 parts of potato bud extracting solutions, 1 to 5 parts of gingko bud extracting solutions, 1 to 5 parts of herba houttuyniae extracting solutions, and 1 to 5 parts of oxalis corniculata extracting solutions. According to the rooting agent for flower cuttage disclosed by the invention, the plant extracting solutions is added, the effects of obviously disinfecting and sterilizing are achieved, the survival rate of the flower cuttage can be obviously improved, and the rooting and adventitious rooting time can be shortened.
Owner:广西鑫雅皇庭园林工程有限责任公司

Method for cutting propagation by utilizing blueberry tissue culture seedlings

The invention discloses a method for cutting propagation by utilizing blueberry tissue culture seedlings. The method disclosed by the invention utilizes the blueberry tissue culture seedlings as cuttings for cutting propagation, combines a rooting culture stage of subculture sprouts in a bottle with transplanting domestication of rooting seedlings, reduces a tissue culture step, saves production costs and greatly shortens rooting time, thereby shortening a seedling raising cycle; and the method explores conditions affecting rooting of cutting of the blueberry tissue culture seedlings through researching influences of different conditions on the rooting of the cutting of the blueberry tissue culture seedlings, thereby improving a rooting rate by utilizing artificial regulation and control.The invention establishes a novel method capable of rapidly reproducing the blueberry seedlings in quantity, and the method provided by the invention can meet heavy demand for blueberries by markets.
Owner:黑龙江朴厚农林科技有限公司

Compound nutrient soil for pot plants

The invention belongs to the technical field of plant nutrient soil, and provides compound nutrient soil for pot plants. The compound nutrient soil is prepared from, by weight, 40-60 parts of pond sludge, 3-12 parts of sepiolite, 5-10 parts of sawdust, 10-15 parts of attapulgite, 5-9 parts of chitosan, 5-11 parts of wormcast, 2-7 parts of compound microorganisms, 1-3 parts of rapeseed dregs, 2-8 parts of calcium superphosphate, 1-3 parts of potassium sulphate, 1-3 parts of rooting power and 0.8-2.1 parts of natural preservative. The compound nutrient soil for the pot plants overcomes the deficiency of existing nutrient soil, and meets the nutrient demand of growth and safety demand of the growth environment of the pot plants, the component and content of the compound nutrient soil are reasonably prepared, and the compound nutrient soil is high in breathability and full in nutrition, resists and prevents bacteria, improves the growth of roots and seedlings, can prevent flower diseases and insect damage, and can promote the healthy growth of the plants.
Owner:HEFEI SHENWO HORTICULTURE CO LTD

Rose cuttings rooting solution and preparation method thereof

The invention discloses a rose cuttings rooting solution. Every liter of solution comprises the following components in parts by weight: 3-6 parts of a GL rooting solution, 20-30 parts of heteroauxin, 2-3 parts of potassium nitrate, 1-2 parts of copper sulfate, 2-4 parts of zinc sulfate, 10-20 parts of ammonium nitrate and 20-30 parts of gibberellin. The rose cuttings rooting solution is reasonable in matching and comprehensive in nutrition and contains microelement required for growth. By utilizing the rose cuttings rooting solution, rooting of rose cuttings is effectively promoted, the rooting time is shortened, and the rooting rate is improved. By means of the microelement, growth of the rose cuttings is promoted, leaves are luxuriant, roots are deep, and then the survival rate of cottage is improved further.
Owner:ZHENJIANG GUANTANG ECOLOGICAL AGRI

Rapid rooting method in blueberry bottle

The invention provides a rapid rooting method in a blueberry bottle, wherein the rooting device includes steps of 1), paving a layer of filter paper in a culture bottle, and pouring the prepared liquid culture medium in the bottle; then sterilizing the culture bottle and a triangular bottle filled with IBA under the temperature of 120-125 DEG C for 15-20 min for future use; 2), selecting thee subculture for 30-40 days, and growing strong blueberry; cutting the blueberry to 3-4 cm long stem and placing the base part in the IBA solution, rapidly dipping for 10-15 s; then vertically placing these stems in the culture bottle paved with the filter paper; 3), culturing for a week under the dark light condition, and then culturing for 20-30 days under the condition that the light intensity is 1500-2500 Lx, and light time length is 10-14h / d. The method is fast in rooting speed, high in rooting rate, simple in operation, and suitable for large-scale production.
Owner:BEIJING VOCATIONAL COLLEGE OF AGRI

Culture method for blueberries

The invention discloses a culture method for blueberries. The method comprises the following concrete steps that (1) before the blueberries are planted, organic fertilizers are uniformly spread on the surface of the soil, the deep turning is carried out for mixing the organic fertilizers into the soil surface layer, and then, sawdust or corn straws are used for coverage; (2) strongly growing new branches are selected, leaves are sheared off, tap water is used for cleanly flushing the branches, then, sterilization is carried out, sterile water is used for rinsing for 3 to 6 times, and in addition, the branches are soaked for 20 minutes in the sterile water; the stem section is sheared into small sections with one to two buds; the small sections are inoculated onto a culture medium for multiplication culture for four weeks, the same method is used for subculture for four times; the stem section base part is inserted into a triangular culture bottle containing rooting substrate, and the temperature, the humidity and the light period in a culture room are controlled; and the transplanting is carried out after one month. When the method provided by the invention is adopted, the rooting rate reaches higher than 90 percent, the rooting time is shortened, the transplanting survival rate reaches 100 percent, and in addition, the speed and the quality of the blueberry seed seedling propagation are greatly and respectively accelerated and improved by the method provided by the invention, so the guarantee is provided for the large-scale commercialized blueberry seed seedling propagation culture.
Owner:MAJIANG QIANYONG BLUEBERRIES

Cuttage method for quick rooting of nepenthes

The invention discloses a cuttage method for quick rooting of nepenthes. The method comprises the following steps that 1, nepenthes branches on nepenthes stock plants are cut off, and cuttage branchesare obtained; S2, the cuttage branches are cut into a medium in a greenhouse, and the medium comprises peat soil, ardealite, silicon powder and nutrient soil; a preparation method of the nutrient soil comprises the following steps that 1, after clam shells are evenly mixed with tourmaline powder, first powder is obtained through calcination; 2, pig manure, wormcast and fermentation liquid are mixed and fermented to obtain yeast; 3, wild radix asparagi, rhizoma arisaematis and garden balsam stem are mixed and put into an ethanol solution, and filtered liquid and filtered residues are obtained;4, the first powder, the yeast, the filtered liquid and the filtered residues are evenly mixed to obtain the nutrient soil. According to the cuttage method of nepenthes, the clam shells are smashed and subjected to multi-segment warming treatment, so that the nutrient soil is good in air permeability and high in water retention and fertilizer retention capacity, it can be guaranteed that nepenthes has sufficient water in the cuttage process, and the survival rate of nepenthes in the cuttage process is increased.
Owner:陈琨

Non-test tube rapid propagation method for Japanese red maple seedling

The invention relates to a non-test tube rapid propagation method for Japanese red maple seedling, which comprises three steps of building a sun greenhouse and a seedling raising tray, preparing exsomatize material, and culturing seed seedling. The method has the beneficial technical effects that the speed of obtaining a complete regeneration plant is rapid and the propagating speed is rapid so that the seed seedling is multiplied quantitatively in short time; the ripening seed seedling has the high purity, does not have pests and has developed and complete root systems; green plantlets inoculated and cultured in the tray have the high purity and do not have pests; the separation of the virus-free seed seedling is propagated; the technology is particularly suitable for the situation that female parent material is few, a great amount of commercial seed seedlings are urgently needed, and the red maples need to be engrafted in the past. After multi-generation circulation technologies, the rootage time is greatly shortened, which is directly related to the childness or young nature of the plants which is aroused; the correct understanding and application of the rapid propagation technology has great significant for accelerating the expanding propagation of the new species and reducing the cost of the seed seedling.
Owner:CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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