The invention provides a plant rooting agent and a preparation method thereof, and belongs to the technical field of agriculture. The plant rooting agent, in parts by weight, is prepared from the following raw materials: 1 to 4 parts of an ABT rooting agent, 6 to 12 parts of glycine, 5 to 10 parts of leucine, 5 to 8 parts of lysine, 3 to 5 parts of phenylalanine, 8 to 10 parts of proline, 5 to 8 parts of aspartic acid, 1 to 4 parts of tea acetic acid, 0.6 to 1.5 parts of indoleacetic acid, 30 to 45 parts of sucrose, 10 to 15 parts of ethyl alcohol, 1 to 3 parts of aspirin, 1 to 3 parts of lentinan, 0.5 to 1 part of B12, 3 to 5 parts of urea, 3 to 5 parts of potassium dihydrogen phosphate, 2 to 6 parts of zinc sulfate, 3 to 5 parts chitosan, 2 to 5 parts of folic acid and 150 to 200 parts of water. The rooting agent provided by the invention is capable of promoting the growing and the rooting of precious seedlings which are difficult to root and improving the quality of the seedlings.