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Open rooting method for sugarcane tissue culture seedlings

An open, tissue-cultured seedling technology, applied in the field of sugarcane cultivation, can solve the problems of increasing labor costs and reducing the production cost of sugarcane tissue-cultured seedlings, and achieve the effects of labor saving, energy saving and high labor efficiency

Active Publication Date: 2015-04-29
广州禾立田生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, labor costs have increased year by year, so it is of great significance to simplify the production process of sugarcane tissue culture seedlings and reduce the production cost of sugarcane tissue culture seedlings

Method used

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  • Open rooting method for sugarcane tissue culture seedlings
  • Open rooting method for sugarcane tissue culture seedlings
  • Open rooting method for sugarcane tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] An open rooting method for sugarcane tissue culture seedlings, comprising the following steps:

[0061] 1) Seedling selection: select sugarcane tissue-cultured clustered seedlings of Xintaitang No. 22 with a seedling height of 5 cm;

[0062] 2) Soaking: Cut off the top leaves of the clustered seedlings, wash the culture medium with tap water, and put it into a glass tissue culture bottle filled with a 1cm-high rooting culture solution. The rooting culture solution contains 1 / 2MS macronutrients, 3mg / LNAA+ IBA 1mg / L and 0.1% carbendazim keep the plants upright;

[0063] 3) Rooting induction: Put the tissue culture bottle containing the seedlings into the artificial greenhouse, keep the temperature at 25-30°C, and light for 10-14 hours / day to induce rooting;

[0064] 4) Bacteriostasis: Step 4) During the induction culture process, spray carbendazim solution containing 0.5% to control the contamination of bacteria, and keep the solution submerged in the base of the plant b...

Embodiment 2

[0067] An open rooting method for sugarcane tissue culture seedlings, comprising the following steps:

[0068] 1) Seedling selection: select sugarcane tissue culture clustered seedlings of black-skinned fruit cane with a seedling height of 5 cm.

[0069] 2) Soaking: Cut off the top leaves of the clustered seedlings, wash the culture medium with tap water, and put it into a glass tissue culture bottle with a 1cm-high rooting culture. LNAA+IBA 1mg / L and 0.5% carbendazim keep the plants upright.

[0070] 3) Rooting induction: put the glass tissue culture bottle containing the seedlings into the artificial greenhouse, keep the temperature at 25-30° C., and light for more than 10 hours to induce rooting;

[0071] 4) Bacteriostasis: step 4) in the process of rooting induction, spraying 0.1% carbendazim solution to control miscellaneous bacteria pollution, keeping the solution submerged in the base of the plant by 1 cm;

[0072] 5) Transplanting: When the plant grows 1 cm long, it ...

Embodiment 3

[0074] An open rooting method for sugarcane tissue culture seedlings, comprising the following steps:

[0075] 1) Seedling selection: select sugarcane clustered seedlings of Yuetang 00-236 with a seedling height of 5 cm.

[0076] 2) Soaking: Cut off the top leaves of the clustered seedlings, wash the culture medium with tap water, and put it into a glass tissue culture bottle filled with a 1cm-high rooting culture solution. The composition of the culture solution is: 1 / 2MS macronutrients, 3mg / LNAA+ IBA1mg / L and 0.3% carbendazim keep the plants upright.

[0077] 3) Rooting induction: put the tissue culture bottle containing the seedlings into the artificial greenhouse, keep the temperature at 25-30°C, and light for more than 10 hours to induce rooting;

[0078] 4) Bacteriostasis: In step 4) during the rooting induction process, spray the carbendazim solution containing 0.4% to control the contamination of bacteria, and keep the solution submerged in the base of the plant by 1 ...

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Abstract

The invention discloses an open rooting method for sugarcane tissue culture seedlings. According to the method, cespititious seedlings in a tissue culture differentiation stage of sugarcane are taken as materials; the cespititious seedlings are induced and rooted by a culture solution to grow in the open environment; the method is characterized by culturing in an open manner and inducing the sugarcane cespititious seedlings to root. The method is simple and liable to implement; the cost can be greatly saved; by virtue of the application of the open culture, the culture does not need to be completed in an artificial super clean bench and high-temperature sterilization culture solution is not required, so that the energy consumption is greatly reduced; meanwhile, by virtue of the open operation, the working efficiency is relative high; the labor is saved; the rooting inducing culture solution is simple in components and free of components such as saccharose and carrageenan, so that the cost is reduced. The open rooting method for sugarcane tissue culture seedlings is high in rooting rate and low in cost, and is applicable to large-scale production of the sugarcane tissue culture seedlings; the workload is greatly relieved; the production cost is reduced.

Description

technical field [0001] The invention relates to a method for cultivating sugarcane, which belongs to the technical field of crop planting, in particular to an open rooting method for tissue cultured sugarcane seedlings. Background technique [0002] Plant tissue culture technology is an important part of plant biotechnology and an effective means for rapid asexual reproduction of plants. It has always been valued by researchers, and has important applications in the propagation of rare plant species and the breeding of excellent plant varieties. With the deepening of research, plant tissue culture technology has gradually improved, and most species have established regeneration systems, which can be used for commercial production. The tissue-cultured seedlings have the excellent traits of maintaining the female parent, and can provide a large number of seedlings with consistent growth in a short period of time, and are suitable for large-scale production, so they are widely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王继华曹干王丽吕冰何琴
Owner 广州禾立田生物科技有限公司
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