Rooting method for tissue culture of Chinese fir

A tissue culture and fir technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as hindering the production process of Chinese fir seedlings, low rooting rate, and difficult rooting of fir

Inactive Publication Date: 2009-07-15
厦门涌泉科技有限公司
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Problems solved by technology

In the process of commercial industrialization of Chinese fir tissue culture, problems such as slow rooting, low rooting rate, and slow growth rate have always hindered the process of industrialized seedling production of Chinese fir
Therefore, the applicant intends to provide a novel Chinese fir rooting medium to solve the problems of difficult rooting and low rooting rate of Chinese fir in the existing Chinese fir culture technology

Method used

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  • Rooting method for tissue culture of Chinese fir
  • Rooting method for tissue culture of Chinese fir

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Embodiment Construction

[0052] In order to better illustrate the present invention, we provide the following examples, the following implementation is only to better illustrate the present invention, but the protection scope of the present invention is not limited thereto.

[0053] The present invention cuts out Chinese fir multiplication seedlings with a length of about 1.5 cm, inoculates them in the Chinese fir rooting medium to carry out tissue culture rooting test, and the rooting mode is cortex rooting type.

[0054] Chinese fir rooting medium consists of different concentrations of NH 4 NO 3 、KNO 3 、KH 2 PO 4 , anhydrous CaCl 2 , ABT1#, IBA, root sun and 185mg / LMgSO 4 ·7H 2 O, 5mg / L ammonium sulfate hydrochloride, 1mg / L niacin, 1mg / L pyridoxine hydrochloride, 100mg / L inositol, 5mg / L ascorbic acid, 0.4mg / L biotin, 15g / L sugar, 0.05g / L activated carbon, trace elements and iron salt composition. Among them, the contents of trace elements and iron salts are the same as those in the traditi...

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Abstract

The invention provides a fir tissue culture rooting method. Small fir multiplication subculture seedlings are cut and transplanted to a fir rooting culture medium for culturing and rooting tissue, and the fir rooting culture medium comprises an improved MS culture medium, 0.4-0.8mg/L of ABT1#, 0.1-0.5mg/L of IBA and 2-6mL/L of root sun diluent. After the fir rooting culture medium is adopted, the rooting time of firs is shortened to 16 to 22 days; after 22 days of culture, the highest rooting rate achieves 96 percent, and nursery stocks grow rapidly so as to decrease the culture cost of the seedlings and realize the industrialized production of the firs.

Description

technical field [0001] The invention relates to a Chinese fir tissue culture rooting method, in particular to a method for tissue culture rooting using the Chinese fir rooting medium composed of an improved MS medium and hormones. Background technique [0002] Chinese fir (Cunninghamia lanceolata (lamb.) Hook) is a species of economic forest with wide adaptability and excellent material quality. Since Chinese fir is a cross-pollination tree species, it is propagated by seeds, and the individual differentiation is large, so it is difficult to maintain the traits of the Chinese fir parent. However, conventional asexual reproduction methods such as cuttings, layering or grafting have slow breeding speeds and are difficult to meet the growing market demand. For this reason, Chinese fir will obtain a large amount of excellent clone seedlings in a short period of time, only by plant tissue culture technology to solve the problem. The tissue culture method is a rapid vegetative p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 赖桂星郑仁华何辉东朱文辉赖春阳郭逸成赖晓昱
Owner 厦门涌泉科技有限公司
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