The invention provides a biological fermentation primary liquid mask and a preparation method and a storing method thereof and belongs to the field of cosmetics. The biological fermentation primary liquid mask is free of a carrier, is attached to the face and free of washing. The biological fermentation primary liquid mask is formed in the mode that blueberries, ginseng, roses, chamomile, soybean and tremella are decocted and fermented. The preparation method comprises the following steps that raw materials are screened, weighed and cleaned in sequence and then mixed, water is added, decocting is conducted, cooling and filtering are conducted, a product is poured into a mould containing original fungi, and then fermentation is conducted for 5-7 days at the temperature of 30-32 DEG C with the humidity being 40%-45% in an air atmosphere; in this way, the biological fermentation primary liquid mask is obtained, wherein the pH value is about 6.8. The storing method of the biological fermentation primary liquid mask comprises the steps that the mask is stored in storage liquid, sealed in vacuum and then stored in a shady, cool and dry place, wherein the storing liquid comprises lemon hydrosol, tea tree hydrosol, lavender hydrosol and mycose. The biological fermentation primary liquid mask achieves the purposes of whitening skin, removing beverage, redness, acne and wrinkles, shrinking pores, eliminating fat granules, improving dark and dry skin, removing dead skin and the like at a time.