Method for reducing aerial root of tissue culture seedling of hydrangea

A technique for hydrangea group and aerial rooting, which is applied in the field of plant tissue culture, can solve problems such as aerial rooting of hydrangea, and achieves the effects of low price, convenient operation and shortening of rooting time.

Inactive Publication Date: 2009-11-18
HUAZHONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for reducing the aerial roots of hydrangea tissue culture seedlings, to solve the difficult problem of producing aerial roots in the rooting process of hydrangea tissue culture seedlings, and to improve the transplanting survival rate of the regenerated plants of hydrangea
[0006] Th

Method used

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  • Method for reducing aerial root of tissue culture seedling of hydrangea
  • Method for reducing aerial root of tissue culture seedling of hydrangea
  • Method for reducing aerial root of tissue culture seedling of hydrangea

Examples

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Example Embodiment

[0049] Example 1

[0050] A: Add 50mL of perlite to the culture flask, and then add 25mL of liquid rooting medium (1 / 2B5 basic medium + indolebutyric acid (IBA) 0.2, 0.5, 1.0 mg / L + sucrose 10g / L1, adjust the pH To 5.8), autoclave at 121°C for 20 min, for use;

[0051] B: Under aseptic conditions, connect the top buds of the hydrangea tissue cultured seedlings (seedling height 1-2cm, with more than 2 leaves) into the prepared rooting medium, and cultivate in the culture room for 30-50 days ( see figure 1 A, figure 2 ).

[0052] C: Take out the tissue culture seedlings of Hydrangea from the perlite, without washing and refining the seedlings, directly transplant the tissue culture seedlings into the substrate with a volume ratio of peat soil: perlite of 1:1;

[0053] The cultivation conditions of the above steps A and B are respectively: the illumination is 1500-2000lx, the illumination time is 16h.d -1 , The culture temperature is 23-25℃;

[0054] The transplanting and cultivation...

Example Embodiment

[0059] Example 2

[0060] The cultivation procedure, cultivation conditions and cultivation management of the cultivation indoor potted plantation are the same as in Example 1.

[0061] The specific steps are:

[0062] A: Add 50mL rooting medium (1 / 2B5 basic medium + IBA with three ratios of 0.2, 0.5, 1.0 mg / L respectively + sucrose 10g / L + agar 7g / L, adjust the pH to 5.8), Autoclave at 121°C for 20min;

[0063] B: Put the top buds of the hydrangea tissue cultured seedlings (seedling height 1-2cm, with 2 or more leaves) into the rooting medium of step A under aseptic conditions on a clean bench, and cultivate 30- in a culture room 50 days;

[0064] C: Remove the hydrangea tissue cultured seedlings from the agar-added rooting medium in step B, wash the root medium, and directly transplant the hydrangea tissue cultured seedlings to peat soil: perlite with a volume ratio of 1:1 In the medium, the survival rate of transplanting can reach about 50%.

[0065] The cultivation conditions ...

Example Embodiment

[0069] Example 3

[0070] The cultivation procedure, cultivation conditions and cultivation management in the cultivation room and pot planting field are the same as in Example 1.

[0071] The specific steps are:

[0072] A: Add about 50mL perlite to the culture flask, and then add 0, 15mL, 25mL, 35mL liquid rooting medium (1 / 2B5 basic medium + IBA 0.5mg / L + sucrose 10g / L, adjust the pH to 5.8), Autoclave at 121°C for 20min;

[0073] B: In the sterile environment of the ultra-clean workbench, put the top buds of the hydrangea tissue cultured seedlings (seedling height 1-2cm, more than 2 leaves) into the rooting medium prepared in step A, and cultivated in the culture room for 30 -50 days (see Figure 4 A, B, C).

[0074] C: Take out the hydrangea tissue culture seedlings from the perlite, do not need to clean and refine the seedlings, directly transplant the hydrangea tissue culture seedlings into the peat soil: perlite volume ratio of 1:1 substrate, the transplantation survival ra...

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Abstract

The invention belongs to the technical field of plant tissue breeding, and in particular to a method for reducing the aerial root of hydrangea, aiming at solving the problem that the aerial root is generated in the tissue culture process of the hydrangea and improving the survival rate of tissue culture seedling of the hydrangea. The method is realized by the following steps: the tissue culture seedling of the hydrangea is taken as an experimental material and is inoculated in a liquid rooting medium (1/2B5+NAA or IBA 0.1-1.0mg/L+sugar 10g/L pH 4-7) of perlite (40-60ml)+25-35ml; after 30 days, no aerial root is generated in the tissue culture seedling, the rooting rate in a culture flask reaches 100%, and the transplanting survival rate reaches 100%. The method not only improves the transplant survival rate and simplifies the operating procedures of transplantation, but also saves the cost of tissue culture by using the perlite to replace agar and recycling the perlite.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for reducing aerial roots of hydrangea tissue culture seedlings, so as to solve the problem of aerial roots in the rooting process of hydrangea tissue culture seedlings and improve the transplanting survival rate of hydrangea tissue culture seedlings . Background technique [0002] Hydrangea macrophylla (Hydrangea macrophylla) Hydrangea family, Hydrangea genus, deciduous shrub, 1-2m high, colorful, large flowers, long flowering period, high ornamental value, is one of the important garden and indoor potted plants. In recent years, with the successful cultivation of miniature potted plants such as 'H.m.'SnowQueen' (H.m.'SnowQueen'), 'Red Bride' (H.m.'Blushing Bride') and other famous new varieties, miniature hydrangea has become more and more popular in domestic and foreign markets , its seedlings are in short supply. Tissue culture technology i...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 王彩云黄丽莉刘峰李扬丽
Owner HUAZHONG AGRICULTURAL UNIVERSITY
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