Tissue culture rapid propagation method for Japanese red maple

A tissue culture and basic medium technology, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of large variation in sowing and reproduction, slow growth, and difficulty in cutting survival, and achieve rapid division, reproduction, and rooting. The effect of shortening the time and reducing the cost of seedlings

Inactive Publication Date: 2009-11-18
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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AI Technical Summary

Problems solved by technology

The scion is obtained by seed propagation, but its sowing propagation has great variation, slow growth, and it is not easy to survive cuttings, which is far from meeting the needs of grafting, which seriously restricts the promotion of Japanese red maple

Method used

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Embodiment Construction

[0022] a. Selection of explants and surface sterilization: cut the young shoots of fine Japanese maple seedlings, cut the stems with axillary buds, rinse the stems with axillary buds in clean water for 1 hour; then wash the explants with 75% alcohol Soak for 40s, then soak in 3% NaClO solution for 15min, and finally rinse with sterilized distilled water for 4 to 5 times, then use as explants for tissue culture;

[0023] b. Induction culture: Under sterile conditions, cut off the browned part of the incision of the explant, and quickly insert the buds of the stem section of the axillary buds upwards on the WPM+0.1mg / L TDZ+0.1mg / L BA induction medium to induce the germination of the axillary buds , and then differentiate into clustered buds; the culture conditions are: temperature 25±2°C, light 14h / D, light intensity 2500-3000Lux;

[0024] c. Proliferation culture: Under sterile conditions, cut the clustered buds into individual plants and insert them into the WPM+0.2mg / L TDZ+0....

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Abstract

The invention relates to a tissue culture rapid propagation method for Japanese red maple which obtains a large quantity of young plants by six steps of the selection and sterilization of explants, the best induction culture medium and growth medium, the preparation of aseptic seedling, the proliferation of sprouts, proliferation growth, rootage culture and hardening-seedling and transplantation. The tissue culture rapid propagation method has the beneficial effects that an asexual propagation technique is utilized for improving the traditional mode of the young plant cultivation. In the human-made optimized environment, the physical optimization difficult problem of the young plants is solved; and various good seed characteristics in plants such as cold-resistant, anti-drought, disease-resistant, insect-resistance, rapid growth and the like are transplanted and optically combined to create new-style fine varieties; the rootage rate of tissue cultured seedlings reaches more than 95%; and the transplantation survival rate reaches more than 85%; the rootage time is greatly shortened, which is directly related to the childness or young nature of the plants which is aroused. The method has the great significant for accelerating the expanding propagation of the new species and reducing the cost of the seed seedling.

Description

technical field [0001] The present invention relates to a kind of tissue culture method, more specifically relate to a kind of tissue culture rapid propagation method of Japanese red maple. technical background [0002] Japanese red maple (Acer palmatum atropurpureum) is a small deciduous tree in the family Aceraceae. It is a variant of Acer palmatum, and its origin is Japan and Korea. Known for its small and delicate tree shape, palmate leaves with various depths of lobes, and brilliant red color. The growth rate of Japanese red maple is much faster than that of ordinary domestic red maple, the color of leaves is brighter, and the cold resistance is stronger. Therefore, Japanese red maple is one of the most widely used varieties of Aceraceae among garden landscape colorful leaf plants. [0003] The existing propagation methods of Japanese maple are mainly seed propagation and grafting. Seed propagation requires harsh cultivation conditions and takes a long time. The graf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 戴传云刘万宏朱蠡庆陈笈陈楠
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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