115results about How to "Rapid multiplication" patented technology

The invention provides a propagation method for grafting Camellia tamdaoensis Hakoda et Ninh onto camellia maozi. The propagation method comprises the steps of carrying out management cultivation of a camellia maozi nursery stock as a stock seed tree and the imported Camellia tamdaoensis Hakoda et Ninh nursery stock as a cutting seed tree, respectively, grafting the Camellia tamdaoensis Hakoda et Ninh cuttings on the camellia maozi cutting seedlings, and controlling the light, the relative humidity and the matrix moisture content in the nursery, thereby obtaining Camellia tamdaoensis Hakoda et Ninh grafted seedlings. The propagation method is characterized in that based on the excellent stock seedling application characteristics of the camellia maozi such as many branches, high germination power, thick and easily strippable bark, and high adaptability to natural environments of drought, flooding, acids, bases, salt and high temperatures, the Camellia tamdaoensis Hakoda et Ninh which is beautiful in leaf shape, flower and crown shape and high in ornamental value and garden development value is grafted on the camellia maozi, the grafting survival rate can be more than 98%, propagation can be expanded rapidly, the propagation coefficient is high, a large quantity of Camellia tamdaoensis Hakoda et Ninh seedlings can be obtained, and after about 5-6 months of management and protection, the grafted seedlings grow as high as 50-60cm.

The invention discloses an indoor breeding method for drosophila-suzukii-matsumura advantageous parasitic wasps trichopria drosophilae.The indoor breeding method includes the following steps that drosophila pupas in the age of 1 day to 2 days are fed and collected, trichopria drosophilae is parasitized to the drosophila pupas (female trichopria drosophilae and male trichopria drosophilae copulate according to the proportion of 1:1), the trichopria drosophilae is collected, and the indoor breeding procedure is completed.The provided expanding propagation method for the trichopria drosophilae is convenient, economical and prone to standard operation, the trichopria drosophilae can be rapidly and largely subjected to expanding propagation, the technological base is provided for industrial production, and the aim for biological control over insect pests is achieved.

The invention discloses a special organic fertilizer for preventing and treating southern blight of jasmine. The special organic fertilizer comprises the following components in parts by weight: 20-25 parts of sheep manure, 12-15 parts of straw, 10-15 parts of pumpkin stem, 5-8 parts of tea leaf residue, 1-2 parts of brown sugar, 5-8 parts of bamboo charcoal powder, 1-2 parts of sea-foam stone powder, 3-5 parts of amino acids, 0.5-1 part of carboxymethylcellulose, 1-2 parts of trichoderma and 0.5-1 part of an EM microbial agent. Due to addition of trichoderma and EM microbial agent in the special organic fertilizer, through rapid propagation of these biological bacteria in soil, plant diseases and insect pests can be effectively inhibited, the special organic fertilizer plays an important role in prevention of the southern blight of jasmine; and after the special organic fertilizer is used in the soil, pesticides can be reduced when diseases appear.

The present invention is two-step explant process for in vitro propagating cut flower lily in high efficiency. After high quality oriental hybrid lily and musk lily is hidden to devernalize, bulb in medium size is selected as initial explant to differentiate directly to proliferate buds. The contagious bud as the secondary explant is proliferated through sound seedling culture, rooting culture, test seedling domestication, transplanting and other steps. The present invention provides effective technological way for providing high quality seedling used in producing cut flower lily and has less problems of seed bulb degeneration, contamination, low propagation speed, etc.

The invention provides a method for treating sewage into reclaimed water by active organisms. The method comprises the following steps: throwing a biological antiseptic disinfectant in sewage, stirring for 10 to 60 seconds, throwing a biological activity purifying agent in the sewage, stirring to obtain recycled water on the upper layer and flocculent settling matters on the lower layer, sending the recycled water on the upper layer to a water circulating system for reuse, periodically discharging the flocculent settling matters on the lower layer, and using the flocculent settling matters as greening fertilizers. Through the addition of the biological activity purifying agent, the leading role of beneficial microorganisms is highlighted, and the capability of the beneficial microorganisms in eating a great number of nutrient substances is enhanced greatly, so that the propagation and the growth of the beneficial microorganisms are accelerated, harmful bacteria in raw wastewater are extinguished quickly due to shortage of food, organic matters in water are absorbed and degraded quickly while the propagation of the beneficial microorganisms is accelerated, lots of impurities in water are absorbed through mucous layers and the capsule structures on the surfaces of the cells of the beneficial microorganisms, and finally the sewage is purified quickly to become recycled water.

The invention discloses a method for water culture cutting propagation of callistemon cirtinus. The method comprises the steps of cutting collection and treatment, nutrient solution preparation, cutting container preparation, water culture, management after cutting and acclimatization and transplant. Current-year semiligneous callistemon cirtinus twigs which grow robustly, have good buds, are free of disease and are selected, undergo trimming treatment and then are inserted in a cutting container filled with nutrient solution to undergo water culture, the culture conditions are controlled, cuttings grown into young roots are obtained and undergo acclimatization and transplant, and callistemon cirtinus seedlings are obtained. The problem that the cutting season is subjected to time limitation is solved, cutting propagation can be performed all the year round, the culture cycle is short, the roots are generated quickly, the environmental controllability is good, the culture conditions are stable, the propagation is quick, and the method is suitable for large-scale production and can be popularized greatly and have good economic benefits, social benefits and ecological benefits.

The invention belongs to the technical field of gardening culture tools and in particular relates to a propagation method for French plum. The invention discloses a propagation method for French plum, comprising the following steps: 1) constructing nursery land; 2) carrying out propagation preparation management; 3) carrying out scion plucking treatment; 4) raising seedlings by cutting; and 5) carrying out maintenance management. The provided propagation method for the French plum has the beneficial effects that cutting survival rate of the French plum can be improved to 80%, and seedling cultivation time is shortened; and the traditional French plum grafting propagation method is changed, and the provided propagation method for the French plum has the advantages of high survival rate, fast propagation, short seedling cultivation time and low cost.

The invention discloses a switchgrass cuttage vegetative propagation method which comprises the following steps: when the switchgrass grows to period with 4-6 knobs, removing the top and leaving 3-4 knobs below the top so as to break the apical dominance and prompt the growth of axillary buds on the knobs below; when lateral buds of 15-20 cm long grow from the axillary buds, cutting the lateral buds together with the parts which are 5 cm long above and below the knobs, so as to be the cuttings; trimming the cuttings and carrying out TDZ (Thidiazuron) rooting powder treatment, and cuttaging the cuttings into a culture medium to carry out rooting culture; and transplanting to a greenhouse or a land after the rooting is accomplished. According to the switchgrass cuttage vegetative propagation method disclosed by the invention, the vegetative propagation is tried on perennial switchgrass of the grass family by using the cuttaging method, and unexpected and good breeding effect is achieved; and the method has the characteristics of being simple and feasible, high in survival rate, unlikely to have variation and the like, and is particularly applicable to providing materials for switchgrass biotechnology study.

The invention discloses a preparation method of a special-purpose fertilizer for preventing and treating actinonema rosae. According to the invention, biological bacteria can be added, enables rapid expanding propagation in soil, can effectively inhibit generation of insect disease, and has important effect on prevention and treatment of actinonema rosae, by applying the fertilizer in soil, the pesticide usage can be reduced when the disease generates, so that China rose pesticide residue can be reduced.

The invention discloses an asexual rapid propagation method for lithocarpus areca. The method comprises the steps of: scion collection; grafting time; grafting; and post-grafting management. The method specifically comprises: selecting a quercus variabilis container seedling as a rootstock; clipping robust and pest-free branches which are from being semi-lignified and lignified from a seed tree of lithocarpus areca as scions; performing grafting by adopting a cleft grafting method; and performing post-grafting management till grafted seedlings of lithocarpus areca obtained in spring in the next year can be discharged out of a garden for afforestation. The method disclosed by the invention is a breakthrough of an asexual propagation technology for lithocarpus areca. By performing propagation by grafting by using the quercus variabilis rootstock, quercus variabilis container seedlings which maintain excellent economical character of a mother parent are massively and rapidly propagated, and the root systems of the seedlings are developed. By virtue of the advantageous characteristic that the quercus variabilis rootstock has a relatively strong adaptive capacity to natural environments such as drought and barren environments, scions and rootstocks of the lithocarpus areca seedlings can be quickly healed and germinate new tips, and the germinated new tips are quick to grow, so that high-quality, high-efficiency and quick development of lithocarpus areca are accelerated. The preparation method has relatively good economical benefit, social benefit and ecological benefit.

The invention discloses a rapid propagation method for tissue culture of wild Thymus Mongolicus Ronn and the application thereof. The method comprises five technical links including obtaining of material, differentiation of adventive buds, rapid proliferation of cluster buds, rooting culture, and seeding exercising and transplant. Based on strict multiple comparative experiment researches, and through the unique innovation of culture conditions, components and proportions of transplant substrates, formulation of culture mediums for different culture stages, and the like, the survival rate can reach about 80%. The invention can be applied to establish a rapid propagation technique system of wild Thymus Mongolicus Ronn for artificial propagation, so that propagation of the wild Thymus Mongolicus Ronn can be accelerated, and the wild Thymus Mongolicus Ronn can be used as an excellent water-saving type landscaping ground cover plant.

The invention discloses a preparation method for special-purpose pesticide fertilizer for controlling black spots of edible roses. According to the invention, biological bacteria which are added are subjected to quick expanding propagation in soil, so that the occurrence of diseases and insect pests can be effectively restrained, and the pesticide fertilizer has quite important action on preventing the black spots of the edible roses; when the pesticide fertilizer is applied into the soil, the usage of pesticide can be reduced if the diseases and pests occur, so that the residue of the rose pesticide is reduced.

The invention discloses a Lithocarpushancei(Benth.)Rehd-QuercusacutissimaCarruth container seedling grafting breeding method comprising the steps of scion acquisition, grafting time, grating, and post-grafting management; selecting QuercusacutissimaCarruth container nursery stock as stock; cutting strong semi lignifications-lignifying branches with no insect disease from Lithocarpushancei(Benth.)Rehd seed tree to serve as scion; using cut-grafting method for grafting; carrying out post-grafting management until next spring so as to obtain Lithocarpushancei(Benth.)Rehd grafted seedlings for afforestation. The method is a breakthrough in Lithocarpushancei(Benth.)Rehd asexual breeding technology; QuercusacutissimaCarruth stock is used for grafting breeding; QuercusacutissimaCarruth container nursery stocks with excellent female parent economic characters are fast and massively breed, and the stock is prosperous in root system; the QuercusacutissimaCarruth stock has strong adaptation capability for natural dry, wet and cold conditions, the Lithocarpushancei(Benth.)Rehd seedling can utilize the advantages, so the scion and stock can fast heal and germinate new shoots; the germinated new shoots can grow fast, thus promoting high quality, efficiency and fast development of the Lithocarpushancei(Benth.)Rehd, and providing better economic benefits, social benefits and ecology benefits.

The invention relates to sinningia speciosa tissue culture propagation and a tuber storage and re-propagation method for sinningia speciosa, wherein the sinningia speciosa tissue culture propagation comprises the steps: culturing a stock plant, selecting and disinfecting an explant, carrying out induced differentiation under the conditions that the culture temperature is 26+/-2 DEG C, the illumination culture time is 11-13 hours per day, and the illumination intensity is 2500-5000lx; carrying out subculture expanding propagation in a 500ml triangular flask; transplanting tissue seedlings, setting the tissue seedlings to obtain sinningia speciosa tissue culture nursery stocks; storing and re-propagating the tissue culture seedlings: namely, culturing propagation-expanded flask seedlings in the 500ml triangular flask under the conditions that the culture temperature is 16-28 DEG C, the illumination intensity is 500-1500lx and the illumination time is 11-12 hours per day, heading in the flask, carrying out tuber subculture storing; and reviving a tuber in the flask and carrying out subculture expanding propagation of the revived tuber in a next period.

The present invention belongs to the field of plant cell engineering seedling culturing technology. The technology of the present invention can raise the absorption capacity of roots of Prunus martima cell engineering seedling, raise the survival rate of Prunus martima cell engineering seedling in hardening off, and lay foundation for the survival, fast rejuvenation and further growth of plant.

The invention relates to a method for killing wood moth larvae and repairing a wormhole. According to the method, bentonite, green muscardine fungi and bacillus thuringiensis are blended into unguent, the blended unguent is filled into a defecation hole for the wood moth larvae by the aid of a grease gun until the unguent overflows from a filling position, the bentonite is solidified to fill the whole wormhole, so that the wood moth larvae die due to asphyxia and mechanical injury, the wormhole is repaired, and the wood moth larvae are killed.

The invention discloses a colorized aglaonema tissue culture method. The method comprises the following steps: selecting healthy colorized aglaonema without insect disease, and stopping watering for several days and then sampling; rinsing by using an aqueous solution with washing powder, then flushing by running water, segmenting and treating by ethanol with concentration of 75% for 30 seconds, disinfecting by 0.1% of HgCl2 for 20 minutes, flushing by sterile water; inoculating the disinfected sterile body for culturing, illuminating for 8 hours/day to obtain an explants; laterally stabbing a growing point by a point of a knife for culturing, illuminating for 8 hours/day; cutting the bud with height greater than 15mm by taking as a single mud form, inoculating in a rooting medium for culturing, illuminating for 12 hours/day; wherein the culture condition is characterized in that light intensity is 1500-2500lx and the temperature is 25-27 DEG C, culturing for 5-6 weeks; and transplanting. By employing the method, the reproducibility is increased to 4.0, the rooting rate is increased to more than 95%, and the plantation survival rate is more than 90%.

The invention discloses a dendrobium officinale tissue culture method based on an enclosed-type plant factory. The method comprises the following steps: setting the environment condition of a tissue-culture room in the enclosed-type plant factory, selecting the tissue culture plants, selecting a medium, setting the illumination intensity and the illumination time of a LED artificial light-supplement lamp of the enclosed-type plant factory, and proportioning the lamp beads of red, blue and far-red light; so that the propagation speed of the dendrobium officinale tissue culture plants is high, tissue culture plants hardening seedling and transplanting survival rate can be increased, and culture period of the tissue culture plants can be shortened.

The invention discloses an asexual rapid propagation method for lithocarpus oleaefolius. The method comprises the steps of: scion collection; grafting time; grafting; and post-grafting management. The method specifically comprises: selecting a quercus variabilis container seedling as a rootstock; clipping robust and pest-free branches which are from being semi-lignified to lignified from a seed tree of lithocarpus oleaefolius as scions; performing grafting by adopting a cleft grafting method; and performing post-grafting management till grafted seedlings of lithocarpus oleaefolius obtained in the next spring can be discharged out of a garden for afforestation. The method disclosed by the invention is a breakthrough of an asexual propagation technology for lithocarpus oleaefolius. By performing grafting propagation by using the quercus variabilis rootstock, quercus variabilis container seedlings which maintain excellent economical characteristics of a female parent are massively and rapidly propagated, and the root systems of the seedlings are developed; By virtue of the advantageous characteristic that the quercus variabilis rootstock has a relatively strong adaptive capacity to natural environments such as drought and barren environments, scions and rootstocks of the lithocarpus oleaefolius seedlings can be quickly healed and germinate new tips, and the germinated new tips are quick to grow, so that high-quality, high-efficiency and quick development of lithocarpus oleaefolius are accelerated. The preparation method has a relatively good economical benefit, social benefit and ecological benefit.

The invention discloses a carrier plant system and a construction method thereof. The carrier plant is wheat, an alternative host is macrosiphum avenae, and a parasitic natural enemy is aphidius gifuensis. The initial macrosiphum avenae inoculating amount is that 200/300 macrosiphum avenae are inoculated in each pot of carrier plants; and the ratio of the female aphidius gifuensis inoculating amount to the number of the macrosiphum avenae during aphidius gifuensis inoculation is 1:40. The method comprises the steps of putting plastic flowerpots with the diameter of 10/30 cm into a greenhouse under the conditions that the room temperature is 25+/-2 DEG C, the humidity is 60+/-10 percent and the photoperiod is 16:8 h, and planting 30/90 plants of wheat in each pot; when the wheat is 5 cm in height, inoculating 200/600 macrosiphum avenae at second instar to third instar; singly putting into plastic covers for one day; inoculating 5/15 female parasitic wasps waiting to oviposit into each cover and shifting out the parasitic wasps after 8 hours. According to the carrier plant system and the construction method thereof, the bee propagation effect is good, the number of the indoor vegetable aphid populations is effectively reduced, and preventive prevention and control, sustainable prevention and control, efficient and comprehensive prevention and control, compatible prevention and control, and low-cost prevention and control are integrated.