Method for promoting exercise survival rate of Prunus maritime cell engineered seedling

A cell engineering and survival rate technology, applied in plant cells, horticultural methods, botanical equipment and methods, etc., can solve the problems of poor root absorption function, easy water loss of seedlings, low photosynthetic capacity of leaves, etc. Improve the survival rate, improve the ability to adapt to various habitats, and enhance the effect of stress resistance

Inactive Publication Date: 2006-09-27
NANJING UNIV
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Problems solved by technology

[0003] (1) The problem to be solved in the present invention is: overcome the problems that the absorption function of the cell engineering seedling root of Lime plum is poor, the conduction function of the stem is weak, the photosynthetic capacity of the leaf is low and the seedling body is relatively tender and weak, and it is very easy to lose water and wilt.

Method used

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Examples

Experimental program
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Embodiment Construction

[0017] ①Collection and identification of arbuscular mycorrhiza (AM mycorrhiza) fungi in the rhizosphere of Binmei plants: 2 were selected from the Yancheng Dafeng Saline Soil Breeding Base, Pukou Breeding Base and Lishui Breeding Base in the Halophyte Laboratory of Nanjing University. Collect and mark the fine-rooted soil of Lime quinquefolium plants above the annual age (the depth of the soil is about 15cm, including the thinnest lateral roots), and bring each sample to the laboratory for wet sieving and decantation to collect the spores of arbuscular mycorrhizal fungi and sporocarps, identified in parallel [refer to the key to Trappe (1982), the "VA Mycorrhizal Fungi Identification Manual" compiled by Schenck et al. Identification].

[0018] ②Purify the above-identified mycorrhizal fungus ("Glomus etunicatum") and multiply it with clover to make a fungal agent;

[0019] ③ After the cell-engineered seedlings of Lime plum with good growth and development and relatively strong...

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Abstract

The present invention belongs to the field of plant cell engineering seedling culturing technology. The technology of the present invention can raise the absorption capacity of roots of Prunus martima cell engineering seedling, raise the survival rate of Prunus martima cell engineering seedling in hardening off, and lay foundation for the survival, fast rejuvenation and further growth of plant.

Description

1. Technical field [0001] The invention belongs to the technical field of plant cell engineering seedling cultivation. 2. Background technology [0002] Binmei is a kind of sandy salt-tolerant shrub fruit tree, native to the Atlantic coast of the United States, distributed from Newfoundland to North Carolina, it can adapt to the unstable substrate and high-salt environment, so it is a One of the important tool species for ecological restoration of coastal degraded ecosystems. It was introduced to my country by Professor Admiral, a doctoral supervisor at the Institute of Biotechnology of Nanjing University, and propagated through cell engineering cloning technology. The woody plant cell engineering seedlings just produced from the tissue culture room have poor absorption function of roots, poor conduction function of stems and low photosynthetic capacity of leaves. Before being cultivated in the field from the tissue culture room, the seedlings must be hardened. The traditi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G7/06C12N5/04
Inventor 钦佩宰学明王光阎道良方逵
Owner NANJING UNIV
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