Colorized aglaonema tissue culture method

A tissue culture and auspicious technology, which is applied in the field of color rough rib auspicious tissue culture, can solve the problems of slow growth, long reproduction cycle and high endophyte contamination.

Inactive Publication Date: 2014-08-13
临沂市农泰农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because this variety is a colorful leaf variety, the chlorophyll content is low, the growth is slow, the propagation cycle of cuttings or divisions is long, and the propagation by tissue culture method has high endophyte pollution, it is less sensitive to hormones, the proliferation rate is low, and it is difficult to take root. Difficulties such as easy withered leaves

Method used

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  • Colorized aglaonema tissue culture method
  • Colorized aglaonema tissue culture method
  • Colorized aglaonema tissue culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: Tissue culture of colored rough rib grass auspicious

[0022] Medium:

[0023] Induction medium (composition of the proliferation medium is the same): improved MS+6BA3.0mg / L +2ip2.0mg / L +KT3.0mg / L +NAA0.1mg / L+triiodobenzoic acid 5.0mg / l+sucrose 40g / l, pH5.8, carrageenan 5.0;

[0024] Rooting medium: 1 / 2 improved MS+NAA0.2mg / L+IBA1.0mg / L+AC0.5g / L+sucrose 30g / L, pH5.8, carrageenan 6.0;

[0025] Drug Name Dosage (mg / l) KNO 3 1300-1900 NH 4 NO 3 1200-1650 MgSO 4 .7H 2 o 370 K H 2 PO 4 170 NaH 2 PO 4 170 CaCl 2 .2H 2 o 220 MnSO 4. 4H 2 o 22.3 ZnSO 4 .7H 2 o 8.6 h 3 BO 3 6.2 KI 0.83 CuSO 4. 5H 2 o 0.025 CoCl 2 .6H 2 o 0.025 FeSO 2 .7H 2 o 27.8 Na 2 —EDTA.2H 2 o 37.3 Glycine 2.0 Thiamine Hydrochloride (Vitamin B1) 0.1 Pyridoxine Hydrochloride (Vitamin B6) 0.5 IVB Niacin 0.5 Inositol 100

[0026]...

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PUM

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Abstract

The invention discloses a colorized aglaonema tissue culture method. The method comprises the following steps: selecting healthy colorized aglaonema without insect disease, and stopping watering for several days and then sampling; rinsing by using an aqueous solution with washing powder, then flushing by running water, segmenting and treating by ethanol with concentration of 75% for 30 seconds, disinfecting by 0.1% of HgCl2 for 20 minutes, flushing by sterile water; inoculating the disinfected sterile body for culturing, illuminating for 8 hours/day to obtain an explants; laterally stabbing a growing point by a point of a knife for culturing, illuminating for 8 hours/day; cutting the bud with height greater than 15mm by taking as a single mud form, inoculating in a rooting medium for culturing, illuminating for 12 hours/day; wherein the culture condition is characterized in that light intensity is 1500-2500lx and the temperature is 25-27 DEG C, culturing for 5-6 weeks; and transplanting. By employing the method, the reproducibility is increased to 4.0, the rooting rate is increased to more than 95%, and the plantation survival rate is more than 90%.

Description

technical field [0001] The invention relates to a method for tissue culture of plants, in particular to a method for tissue culture of colored Grass auspiciousness. Background technique [0002] auspicious, latin name Aglaonema ‘Favonian’ , Aroidaceae, Sclerosia genus. The leaves are elliptic, acuminate, and the leaves are pink with green spots or spots. Hi warm and humid and semi-shady environment. Not cold-resistant, afraid of strong light exposure, not drought-tolerant. Propagate by division or cuttings, spring to autumn is the most suitable period. Because this variety is a colorful leaf variety, the chlorophyll content is low, the growth is slow, the propagation cycle of cuttings or divisions is long, and the propagation by tissue culture method has high endophyte pollution, it is less sensitive to hormones, the proliferation rate is low, and it is difficult to take root. Difficulties such as easy withered leaves. Contents of the invention [0003] The purpose o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 林文革林春霞刘永淦刘丽涂艺辉
Owner 临沂市农泰农业科技有限公司
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