Tissue culture rootage and transplantation methods for wild thymus mongolicus ronn
A technology of tissue culture and thyme, which is applied in the field of transplanting and rooting of wild Mongolian thyme tissue culture, can solve the problem that there is no method for rapid propagation of wild Mongolian thyme, and achieve the effect of accelerated propagation
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[0019] Rooting culture: Inoculate the seedlings with a height of 1-2cm on the rooting medium to induce rooting. The formula of the medium is: MS+sucrose 30g / L+agar 6g / L+IBA 0.7mg / L, pH5.8-6.0, after 4 days Rooting begins, and the rooting rate reaches 100%.
[0020] Seedling hardening and transplanting: cultivate on the rooting medium for 26-27 days, grow to more than 3cm, remove the sealing film and open the bottle to harden the seedlings for 3-4 days, take out the tissue culture seedlings, take clumps as a unit (5-7 plants per clump) ), wash the culture medium on the root, dip in 0.5% potassium permanganate solution for 5-10s, and transplant to the nutrient soil (peat: perlite: vermiculite=2: 1:1) in a nutrient bowl, maintain a temperature of about 25°C and an air humidity of about 80%, spray water 3-4 times a day, and survive in about 14 days, with a survival rate of about 80%.
[0021] The wild Mongolian thyme (Thymus mongolicus Ronn.) tissue culture rooting and transplant...
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