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Chuanminshen violaceum tissue culture rapid propagation technology

A technology of ginseng and tissue culture, applied in the field of plant tissue culture, can solve the problem that there is no ginseng tissue culture and in vitro rapid propagation technology, restricting the improvement of large-scale planting output value of ginseng, insufficient seedlings and cultivation costs, etc. problem, to achieve the effect of fast reproduction, easy operation and short induction time

Inactive Publication Date: 2012-11-28
四川绿地景泰农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The low germination rate leads to insufficient seedlings and high cultivation costs, and it is difficult to guarantee the quality of seedlings, which restricts the development of large-scale planting of Chuanming ginseng and the increase of output value per unit area
At present, there is no tissue culture and in vitro rapid propagation technology of Chuanming ginseng

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Selection and sterilization of explants: Select the young leaves of Chuanming ginseng in the vigorous growth period as explants, wash them with running water for 30 minutes, soak them in 75% alcohol for 30 seconds on the ultra-clean workbench, and use sterile deionized Rinse with water for 3 times, then place the washed explants in 0.1% mercuric chloride solution, immerse and sterilize for 10 minutes, rinse with sterile deionized water for 3 times, dry the surface water with sterile filter paper, and set aside.

[0029] 2. Explant inoculation: cut the spare explant in step 1 into 0.5cm2 size on the ultra-clean workbench, and inoculate it in the callus induction medium (the medium is: 1 / 2MS+6-BA1. 0mg / L+2,4-D10.0mg / L+NAA5.0mg / L, add 30g / L sucrose, 8g / L agar powder, pH=5.8), at constant temperature 25±2℃, light (2000lx) for 14h The culture was carried out under the condition of / d, and after one week, the middle of the young leaves arched, and after continuous culture ...

Embodiment 2

[0036]1. Selection and sterilization of explants: Select the fresh young petioles of Chuanming ginseng in the vigorous growth period as explants, wash them with running water for 20 minutes, soak them in 75% alcohol for 30 seconds on the ultra-clean workbench, and remove them with aseptic. Rinse with deionized water for 3 times, then place the washed explants in 0.2% mercuric chloride solution, soak and sterilize for 10 minutes, rinse with sterile deionized water for 3 times, dry the surface water with sterile filter paper, and set aside .

[0037] 2. Explant inoculation: Cut the spare explants in step 1 into small pieces of 0.5-1 cm on the ultra-clean workbench, and inoculate them in the callus induction medium (the medium is: 1 / 2MS+6 -BA3.0mg / L+2,4-D20.0mg / L+NAA1.0mg / L, add 30g / L sucrose, 8g / L agar powder, pH=5.8), at constant temperature 25±2℃, light ( 2000Lx) under the condition of 14h / d, one week later, the middle of the petiole and the incision swelled, and after contin...

Embodiment 3

[0044] 1. Selection and sterilization of explants: Select the stem tips of Chuanming ginseng in the vigorous growth period as explants, wash them with running water for 20 minutes, soak them in 75% alcohol for 20 seconds on a super-clean workbench, and wash them with sterile deionized water. Rinse 3 times, then place the washed explants in 0.2% mercuric chloride solution, immerse and sterilize for 10 minutes, rinse 3 times with sterile deionized water, dry the surface water with sterile filter paper, and set aside.

[0045] 2. Explant inoculation: peel off the attached leaves on the ultra-clean workbench of the spare explant in step 1 to obtain a smooth shoot tip, and inoculate it in the callus induction medium (the medium is: 1 / 2MS+ 6-BA2.0mg / L+2,4-D10.0mg / L+NAA5.0mg / L, add 30g / L sucrose, 8g / L agar powder, pH=5.8), at constant temperature 25±2℃, light (2000Lx) under the condition of 14h / d. After one week, the middle of the young leaves arched, and the middle of the petiole an...

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Abstract

The invention provides a Chuanminshen violaceum tissue culture method. According to the method, the stem tip, tender leaves and fresh tender leaf stalks of the Chuanminshen violaceum plant with good characters in the vigorous growth period are utilized as explants, and the method comprises the following steps of: performing disinfection treatment and inoculation induction to form callus; performing callus multiplication, bud induction culture and rooting culture to obtain complete Chuanminshen violaceum plant seedling; and transplanting the complete seedling into a seedling culture medium, and culturing to obtain a Chuanminshen violaceum annual seedling. All the culture media are based on an MS culture medium in combination with components such as 6-benzyladenine, naphthylacetic acid, 2,4-dichlorphenoxyacetic acid, indolebutyric acid, sucrose an agar. The method provided by the invention can be used for realizing the tissue rapid propagation of Chuanminshen violaceum by use of a plant tissue culture technology, and has important application values in terms of annual seedling supply and improved-variety rapid expanding propagation of the industrialized cultivation of Chuanminshen violaceum.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and mainly relates to a tissue culture method of Chuanming ginseng. Background technique [0002] Chuanming ginseng ( Chuanminshen violaceum Sheh et Shan) is a perennial herb of the Umbelliferae family (Umbelliferae). It is a unique single-species plant in my country and is an authentic medicinal material in Sichuan. It is distributed along a narrow strip from central and eastern Sichuan (Jintang, Jianyang, Bazhong, Langzhong, southern) to western Hubei (Yichang, Dangyang), and is most concentrated in Jintang, Cangxi, Langzhong, and Bazhong of Sichuan. "Records of Traditional Chinese Medicine" (1959), "Records of Sichuan Traditional Chinese Medicine" and so on once recorded Chuanmingshen and Mingdangshen as the same plant. Established the genus Chuanming Shen, and believed that the two genera should belong to different families. Lu Yin et al. used gas chromatography-mass spectrom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙辉龚艺
Owner 四川绿地景泰农业有限公司
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