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Sinningia speciosa tissue culture propagation and tuber storage and re-propagation method

A technology for tissue culture and tissue culture seedlings, applied in the field of plant cultivation, can solve the problems of low success rate of disinfection and inoculation of the explants of C. cost reduction effect

Inactive Publication Date: 2014-06-18
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Over the past 20 years, there have been more than 100 domestic research papers on gloxinia tissue culture, but most of the research stays at the laboratory stage
In the case of no large orders for 1 to 2 years, it is quite expensive to ask workers to prepare culture medium, sterilize, and subculture to preserve sterile in vitro propagation materials; on the other hand, the success rate of disinfection and inoculation of gloxinia explants It is very low, and the acquisition of inoculation materials is affected by the outdoor temperature and the growth status of potted seedlings; when market demand reappears, it often takes a long time to form a production scale from re-inoculation, and it is difficult to meet market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Choose the potted gloxinia that has bloomed or is blooming as the mother plant, topdress and trim appropriately to promote the germination of gloxinia and the growth of the plant; choose the robust axillary buds or terminal buds of gloxinia with lower branches as explants, cut into 3~ 4 cm, infiltrated with 70% ethanol for 10 seconds on the ultra-clean workbench, rinsed twice with sterile water, then transferred the material to a sterilized jar, sterilized with 0.1% mercuric chloride for 10 minutes, Finally, wash 5 times with sterile water.

[0029] Cut off 2-3 mm incisions at both ends of the sterilized explants, and insert the trimmed explants obliquely into the test tube with NH 4 NO 3 1200 mg / L, KNO 3 1600 mg / L, CaCl 2 400 mg / L, MgSO 4 370 mg / L, KH 2 PO 4 170 mg / L, H 3 BO 3 0.63 mg / L, MnSO 4 4H 2 O 2.23 mg / L, ZnSO 4 ·7H 2 O 15 mg / L, Na 2 MoO 2 2H 2 O 0.025 mg / L, CuSO 4 ·5H 2 O 0.0025 mg / L, FeSO 4 ·7H 2 O 27.8 mg / L, Na 2 EDTA 2H 2 O 37.3 mg...

Embodiment 2

[0032] Choose the potted gloxinia that has bloomed or is blooming as the mother plant, topdress and trim appropriately to promote the germination of gloxinia and the growth of the plant; choose the robust axillary buds or terminal buds of gloxinia with lower branches as explants, cut into 3~ 4 cm, infiltrated with 70% ethanol for 10 seconds on the ultra-clean workbench, rinsed twice with sterile water, and then transferred the material to a sterilized jar, sterilized with 0.1% mercuric chloride for 11 minutes, Finally, wash 5 times with sterile water.

[0033] Cut off 2-3 mm incisions at both ends of the sterilized explants, and insert the trimmed explants obliquely in the NH 4 NO 3 1200 mg / L, KNO 3 1600 mg / L, CaCl 2 400 mg / L, MgSO 4 370 mg / L, KH 2 PO 4 170 mg / L, H 3 BO 3 0.63 mg / L, MnSO 4 4H 2 O 2.23 mg / L, ZnSO 4 ·7H 2 O 15 mg / L, Na 2 MoO 2 2H 2 O 0.025 mg / L, CuSO 4 ·5H 2 O 0.0025 mg / L, FeSO 4 ·7H 2 O 27.8 mg / L, Na 2 EDTA 2H 2 O 37.3 mg / L, inositol ...

Embodiment 3

[0036] Choose the potted gloxinia that has bloomed or is blooming as the mother plant, topdress and trim appropriately to promote the germination of gloxinia and the growth of the plant; choose the robust axillary buds or terminal buds of gloxinia with lower branches as explants, cut into 3~ 4 cm, infiltrated with 70% ethanol for 9 seconds on the ultra-clean workbench, rinsed twice with sterile water, then transferred the material to a sterilized jar, sterilized with 0.1% mercury liter for 12 minutes, Finally, wash 5 times with sterile water.

[0037] Cut off 2-3 mm incisions at both ends of the sterilized explants, insert the trimmed explants obliquely into the above-mentioned modified ER formula, add BA 0.5 mg / L, NAA 0.25 mg / L, On the test tube induction medium with 30 g / L sucrose and 4.5 g / L agar, only one explant was inserted in each test tube for induction culture. 4000 lx, the explants expanded after 15 days, and buds appeared after 25 days; then the aseptic material th...

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Abstract

The invention relates to sinningia speciosa tissue culture propagation and a tuber storage and re-propagation method for sinningia speciosa, wherein the sinningia speciosa tissue culture propagation comprises the steps: culturing a stock plant, selecting and disinfecting an explant, carrying out induced differentiation under the conditions that the culture temperature is 26+ / -2 DEG C, the illumination culture time is 11-13 hours per day, and the illumination intensity is 2500-5000lx; carrying out subculture expanding propagation in a 500ml triangular flask; transplanting tissue seedlings, setting the tissue seedlings to obtain sinningia speciosa tissue culture nursery stocks; storing and re-propagating the tissue culture seedlings: namely, culturing propagation-expanded flask seedlings in the 500ml triangular flask under the conditions that the culture temperature is 16-28 DEG C, the illumination intensity is 500-1500lx and the illumination time is 11-12 hours per day, heading in the flask, carrying out tuber subculture storing; and reviving a tuber in the flask and carrying out subculture expanding propagation of the revived tuber in a next period.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to rapid propagation of ornamental flowers and gene preservation of sterile materials, in particular to a method for tissue culture propagation of gloxinia, tuber preservation and repropagation. Background technique [0002] Gloxinia ( Sinningia speciosa ) is a perennial fleshy bulbous herbaceous flower of the genus Gloxinia of Gesneriaceae. It is native to the tropical rain forest of Brazil and was introduced into my country in the 1930s. The tubers of gloxinia are oblate and spherical, the stems on the ground are very short, the plant height is 15-25 cm, the whole plant is densely covered with white fluff, the flowers are large and colorful, and the flowering period is long. It is an ideal flower for indoor potted plants, flower beds, and conference venues. Gloxinia is not cold-resistant, and the aboveground part of the plant will die in winter. Underground tubers need v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄小荣闫鼎羽彭玉华申文辉庞世龙谭一波钟瑜
Owner GUANGXI FORESTRY RES INST
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