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Method for cultivating subcultured bud of upright crown tissue culture seedling of fir wood

A cultivation method and a technology of subculture buds, which are applied in the field of subculture bud cultivation of Chinese fir crown tissue culture seedlings, can solve problems such as partial crowns of Chinese fir tissue seedlings, and achieve the effects of large seedling cultivation, fast propagation speed, and removal of position effects

Active Publication Date: 2014-11-19
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In order to overcome the deficiencies in the prior art, provide a kind of problem that can solve the partial crown of Chinese fir tissue seedlings, and develop the cultivation method of the secondary buds of the Chinese fir crown tissue culture seedlings that develop the high-efficiency positive crown tissue culture and rapid propagation technology of Chinese fir fine clones

Method used

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  • Method for cultivating subcultured bud of upright crown tissue culture seedling of fir wood
  • Method for cultivating subcultured bud of upright crown tissue culture seedling of fir wood
  • Method for cultivating subcultured bud of upright crown tissue culture seedling of fir wood

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Embodiment 1

[0035]The invention discloses a method for cultivating subculture buds of Chinese fir crown tissue culture seedlings, in which excellent clones are selected to graft young shoots of Chinese fir saplings in the current year as explants. Cut off the leaves on the explants, gently brush them with a brush in the detergent solution, soak them in 0.1% chloroquine for 40 minutes, and then rinse them with pure water for 3 to 5 times. Cut into 1-2 cm long stem segments, and disinfect the cut explants with 75% ethanol in a ultra-clean workbench for 10 seconds. When the explant belongs to the young shoot tip, place the sterilized explant in the ascorbic acid of 10mg / L and soak for 5min to sterilize; The explants were sterilized by immersing in 30 mg / L ascorbic acid for 8 minutes; when the explants belonged to lignified stem segments, sterilized by immersing the sterilized explants in 30 mg / L ascorbic acid for 12 minutes. The sterilized explants were then inoculated into the initial medi...

Embodiment 2

[0038] The invention discloses a method for cultivating subculture buds of Chinese fir crown tissue culture seedlings, in which excellent clones are selected to graft young shoots of Chinese fir saplings in the current year as explants. Cut off the leaves on the explants, gently scrub them with a brush in the detergent solution, soak them in 0.1% chloroquine for 45 minutes, and then rinse them with pure water for 4 to 5 times. Cut into 1-2 cm long stem segments, and disinfect the cut explants with 75% ethanol in a ultra-clean workbench for 10 seconds. When the explant belongs to the young shoot tip, place the sterilized explant in the ascorbic acid of 10mg / L and soak for 5min to sterilize; The explants were sterilized by immersing in 30 mg / L ascorbic acid for 8 minutes; when the explants belonged to lignified stem segments, sterilized by immersing the sterilized explants in 30 mg / L ascorbic acid for 12 minutes. The sterilized explants were then inoculated into the initial med...

Embodiment 3

[0041] The invention discloses a method for cultivating subculture buds of Chinese fir crown tissue culture seedlings, in which excellent clones are selected to graft young shoots of Chinese fir saplings in the current year as explants. Cut off the leaves on the explants, gently scrub them with a brush in the detergent solution, soak them in 0.1% chloroquine for 45 minutes, and then rinse them with pure water for 4 to 5 times. Cut into 1-2 cm long stem segments, and disinfect the cut explants with 75% ethanol in a ultra-clean workbench for 10 seconds. When the explant belongs to the young shoot tip, place the sterilized explant in the ascorbic acid of 10mg / L and soak for 5min to sterilize; The explants were sterilized by immersing in 30 mg / L ascorbic acid for 8 minutes; when the explants belonged to lignified stem segments, sterilized by immersing the sterilized explants in 30 mg / L ascorbic acid for 12 minutes. The sterilized explants were then inoculated into the initial med...

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Abstract

The invention discloses a method for cultivating a subcultured bud of an upright crown tissue culture seedling of fir wood. The method comprises the following steps: an excellent clonal annual burgeon of the fir wood is used as an explant, a breeding bud is obtained through disinfection, sterilization and tissue culture, the breeding bud is innoculated in a proliferation culture medium comprising 1 / 2MS, 1.0mg / L of 6-BA and 0.5mg / L of IBA for inducing the cluster buds to sprout, the subcultured bud which is germinated at the base of the breeding bud and is healthy in growth is selected and is cut in a common rooting culture medium, seedlings are exercised and transplanted, and when the height of the seedlings of the fir wood is 15-30 cm, the seedlings are taken out of a garden for forestation. When the method is used, the proliferation rate of the subcultured bud of the fir wood is as high as 4-6, the breeding speed is high, the seedling culturing number is large, the tissue culture subcultured bud with high genetic stability, 100% of the upright crown rate of the tissue culture seedling and more than 96% of the rooting rate can be in batch production in a short period, and the requirements of the construction and the development of a fir wood reserving base of China for good seedlings can be met, so that the method has better economic benefits, social benefits and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of asexual rapid propagation of plants, and in particular relates to a method for cultivating secondary buds of Chinese fir crown tissue culture seedlings. Background technique [0002] Chinese fir (Cunninghamia Lanceolata) belongs to the family Taxodiaceae and belongs to the genus Cunninghamia. It is a unique fast-growing commercial timber tree species in my country, with fast growth and good material quality. The wood texture is straight, the structure is uniform, and it is not warped or cracked. The material is light and tough, with moderate strength and high quality coefficient. It has a fragrance, and the wood contains "Shannao", which can resist insects and rot, and is easy to process. Widely used in construction, furniture, appliances, shipbuilding and other aspects. [0003] my country is not only a large timber production country, but also a large timber consumption country. After the implement...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 吴幼媚蔡玲戴春香黄金使韦颖文王以红
Owner GUANGXI FORESTRY RES INST
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