Method for accelerating generation adding of brassica campestrisssp.chinensis makino

A fast technology for heading Chinese cabbage, which is applied in the directions of seed and rhizome treatment, application, leaf crop cultivation, etc. It can solve the problems affecting the reproductive efficiency of non-heading cabbage, the growth period of non-heading cabbage, and the improvement, and achieves The effect of rapid multigenerational breeding and improving breeding efficiency

Active Publication Date: 2019-02-22
WUHAN ACADEMY OF AGRI SCI
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  • Description
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Problems solved by technology

Previous attempts have been made to propagate non-heading cabbage materials by means of off-site shuttle breeding, winter greenhouse seed collection, and tissue culture, but these methods can only achieve a reproductive efficiency of 1.5-2 generations a year
The reason is that non-heading cabbage has a long growth period, and the vegetative growth stage needs to feel the influence of vernalization. In the later stage, it needs to sense higher temperature and long sunshine to bloom normally, and even mature seeds have a strong dormancy phenomenon. , so the superposition of these factors affects the improvement of the reproductive efficiency of non-heading Chinese cabbage

Method used

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  • Method for accelerating generation adding of brassica campestrisssp.chinensis makino
  • Method for accelerating generation adding of brassica campestrisssp.chinensis makino
  • Method for accelerating generation adding of brassica campestrisssp.chinensis makino

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Embodiment Construction

[0023] Below in conjunction with embodiment the present invention is described in further detail.

[0024] There are two non-heading Chinese cabbage materials used in this example: one is the inbred line 'QR2' (Wuhan Academy of Agricultural Sciences Vegetable Selection) with strong winter sex, and the other is the variety 'Han Excellent' (Wuhan Academy of Agricultural Sciences Vegetable Breeding).

[0025] 1. Seed treatment

[0026] 1.1 Seed germination: Select 20 seeds of non-heading Chinese cabbage 'QR2' and 'Hanyou' with full grains, put them in a petri dish respectively, put a layer of filter paper on the bottom of the dish, add a little pure water to make the seeds It is advisable to fully imbibition without affecting its respiration. Put the petri dish in a 25°C incubator to observe the germination state of the seeds.

[0027] 1.2 Vernalization treatment time test: 2 days later, the seeds of 'QR2' and 'Hanyou' showed white buds, but the cotyledons had not yet unfolded...

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Abstract

The invention discloses a method for accelerating generation adding of brassica campestrisssp.chinensis makino. The method comprises the following steps that after seed germination, seeds are vernalized at 2-4 DEG C for 15-25 days, then seedlings are transplanted into a greenhouse to be subjected to all-day illumination cultivation under full-spectrum LED lamp tubes, after plants produce buds, theplant type is controlled by removing peripheral old leaves and rosette leaves, after main stalks blossom, mature flower buds at the outermost periphery of the inflorescence are pollinated, seed coatsof immature seeds after pollination are peeled to break the dormancy, and after sowing, brassica campestrisssp.chinensis makino enters the next generation. By means of the method, the breeding progress of the new variety of brassica campestrisssp.chinensis makino can be greatly accelerated, and it is achieved that brassica campestrisssp.chinensis makino has at least four breeding generations every year.

Description

technical field [0001] The invention belongs to the field of crop breeding, and in particular relates to a new method for realizing fast generation of non-heading Chinese cabbage in a greenhouse. Background technique [0002] Non-heading cabbage is a biennial cruciferous vegetable, which can only reproduce one generation a year under natural conditions. It generally takes 6-8 years to develop an inbred line in the breeding process, and even longer to develop a hybrid. In order to improve the breeding efficiency of non-heading Chinese cabbage, it is urgent to shorten its growth cycle and explore the method of fast generation. The predecessors have tried off-site shuttle breeding, winter greenhouse seed collection and tissue culture to propagate non-heading Chinese cabbage materials, but these methods can only reach the reproductive efficiency of 1.5-2 generations a year. The reason is that non-heading cabbage has a long growth period, and the vegetative growth stage needs t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/15A01C1/00
CPCA01C1/00A01G22/15
Inventor 高长斌汪爱华宋莉萍王斌才
Owner WUHAN ACADEMY OF AGRI SCI
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