Rapid propagation method for tissue culture of wild Thymus Mongolicus Ronn and application thereof

A technology of tissue culture and thyme, which is applied in the fields of application, horticultural methods, botanical equipment and methods, etc., can solve the problems that there is no method for rapid propagation of wild Mongolian thyme, and achieve the effect of accelerated multiplication

Inactive Publication Date: 2012-03-28
北京市园林学校
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  • Abstract
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Problems solved by technology

[0003] At present, there is no practical method for rapid propagation of wild Mongolian thyme

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment

[0035] Culture conditions: culture temperature 24±2℃, illuminance 3000Lux, light quality is fluorescent lamp, light 12h.d -1 .

[0036] Acquisition of sterile materials: select plants that grow robustly and are free from diseases and insect pests, cut off the stems with axillary buds, rinse the surface, soak in washing spirit solution for 10 minutes, rinse with running water for 30 minutes, and soak in 75% alcohol for 30 seconds on the ultra-clean workbench Disinfect, rinse with sterile water 3 times, then soak in 0.1% mercuric chloride for 8 minutes, rinse with sterile water 5 times, cut off the parts in contact with the liquid medicine, and set aside.

[0037] Differentiation of adventitious buds: inoculate sterile stem segments on cluster bud induction medium, the formula of the medium is: MS+sucrose 30g / L+agar 7g / L+6-BA 0.5mg / L+IAA0.1mg / L, pH5 .8-6.0, after 14 days of culture, small buds grow from the axillary buds, and more clustered buds differentiate on 21 days, and ea...

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Abstract

The invention discloses a rapid propagation method for tissue culture of wild Thymus Mongolicus Ronn and the application thereof. The method comprises five technical links including obtaining of material, differentiation of adventive buds, rapid proliferation of cluster buds, rooting culture, and seeding exercising and transplant. Based on strict multiple comparative experiment researches, and through the unique innovation of culture conditions, components and proportions of transplant substrates, formulation of culture mediums for different culture stages, and the like, the survival rate can reach about 80%. The invention can be applied to establish a rapid propagation technique system of wild Thymus Mongolicus Ronn for artificial propagation, so that propagation of the wild Thymus Mongolicus Ronn can be accelerated, and the wild Thymus Mongolicus Ronn can be used as an excellent water-saving type landscaping ground cover plant.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation technology of wild ground cover plants, in particular to a tissue culture rapid propagation method and application of wild Mongolian thyme. Background technique [0002] China is rich in wild thyme resources. In China, it is mainly distributed in dry and sunny environments such as rocky mountains and sandy land at an altitude of about 1100-3600m in the "Three Norths" region. Mongolian thyme (Thymus mongolicus Ronn.) is a plant of the genus Thymus in the Labiatae family. It is a perennial dwarf shrub. It is often distributed in the form of a carpet in the wild. The branches, leaves and flowers have a strong fragrance. It has strong drought resistance and can be used as an excellent water-saving landscaping material in urban gardens and green spaces. [0003] At present, there is no practical method for rapid propagation of wild Mongolian thyme. Contents of the invention [0004] The object ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G1/00
Inventor 徐荣马玉吴宗凯刘广亮贾建中唐进群吴岩于涵刘冬梅陈战是王玉圳
Owner 北京市园林学校
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