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Method for constructing artificial algal crusts by utilizing mixed algae

A technology of algae and crust, applied in the fields of botanical equipment and methods, application, seaweed cultivation, etc., can solve the problems of large investment and long cultivation process cycle, and achieve the effect of huge application potential.

Inactive Publication Date: 2012-08-01
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the restoration of artificial algal crusts, most domestic phycologists firstly purify and separate the algal species, cultivate them on a large scale, and then inoculate several dominant algal species to construct algal crusts (Hu Chunxiang et al. Quicksand fixation method, 2004), this method has also achieved good sand fixation effect in the field, but it has disadvantages in the separation of algae species, long period of cultivation process, and large investment.

Method used

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  • Method for constructing artificial algal crusts by utilizing mixed algae
  • Method for constructing artificial algal crusts by utilizing mixed algae
  • Method for constructing artificial algal crusts by utilizing mixed algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Using mixed algae to construct artificial algae crust

[0020] The present invention specifically provides a method for constructing an artificial algae crust by using mixed algae, and its specific construction method steps are as follows:

[0021] (1) Preparation of inoculation materials: select fully developed algal crusts in the desert, collect them, air-dry them, grind them, and then collect soil samples. The algae contained in the soil samples were dominated by Microcoletheca, Coleopsis, Matella, Oscillatoria, Nostoc, Monofidophyta, and Pseudocladon filamentous cyanobacteria, among which Microcoletheca was the dominant species. , Chlorella terrestris, Chlorella, Chromococcus and other common species of spherical algae.

[0022] (2) Cultivation: Insert the prepared BG into the culture plate 11 For liquid culture medium, pass the bare sand through a 0.2mm sieve and add 100g of bare sand to the medium per liter of medium, add bare sand to cover the bottom...

Embodiment 2

[0027] Embodiment 2: The present invention utilizes mixed algae to construct the effect test of artificial algae crust

[0028] The content of chlorophyll a in the soil is used to represent the algae biomass. For the measurement method, refer to the literature (Xie et al., 2007, Soil Biology & Biochemistry, 567-572). The biomass of the harvested algae is the product of the biomass content of the algae and the soil weight of the harvested algae.

[0029]

[0030] The method for constructing artificial algae crusts provided by the present invention can rapidly proliferate algae species, and the natural algae crusts collected from the field have an average biomass of 0.016 ± 0.005mg chlorophyll a / gram of dry soil, in BG 11 After 2-3 weeks of indoor cultivation in liquid medium, the biomass of algae species and sand grains is 0.663±0.054 mg chlorophyll a / gram of dry soil after collection and drying, and the biomass has increased by as much as 124 times.

Embodiment 3

[0031] Embodiment 3: The present invention utilizes mixed algae to construct the effect test of artificial algae crust

[0032] In this experiment, four 2.5m×2.5m squares were set up in the greenhouse, covered with quicksand with a thickness of 10cm, and then treated with different algae species:

[0033] Microsheatha

[0034] B: The common dominant algae species in several biological crusts are mixed in a regular ratio

[0035] C The mixed algal species formed by natural algal crusts through liquid culture

[0036] D: Natural algal crust alga species.

[0037] After the algae were ground, the chlorophyll content was measured, and the algae biomass was converted to be equal, and the algae whose total biomass was 100 mg chlorophyll a were used for inoculation, and other conditions were the same. After cultivating for 3 months, soil samples were collected at a depth of 0.5 cm, and the content of chlorophyll a was determined after natural drying. The thickness of the formed a...

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Abstract

The invention discloses a method for constructing artificial algal crusts by utilizing mixed algae. The method comprises the following steps of: selecting sufficiently developed algal crusts on the desert, collecting the algal crusts, and grinding the algal crusts after air-drying the algal crusts; sieving bare sand with a 0.2mm sieve, then adding 1 liter of BG11 liquid culture medium to 100g of bare sand, and collecting the algae together with the grains of sand fixed on the algae three weeks later, wherein the inoculum size is 50g dry soil per liter of culture medium, the culture temperature is the room temperature, the illumination intensity is 4000lux, and in the cultured mixed algae, the dominant species are filamentous algae which are mainly microcoleus, lyngbya and phormidium and account for 80% of the total biomass, and species such as oscillatoria, chlorococcum humicola, chlorella, chlamydomonas, Navicula, hantzschia and the like account for 20% of the total biomass; grindingthe collected algae into alga powder after air-drying the collected algae, and putting the alga powder in a refrigerator at minus 20 DEG C; and carrying out inoculation on the bare sand when the soiltemperature is 20 DEG C, wherein the inoculum size is 80g.m<-2>, and the moisture applied daily is 3L.m<-2>. The method successfully avoids the problems of purification and separation of the algae, and has wide applicability.

Description

field of invention [0001] The invention relates to the biotechnical field of constructing artificial biological crusts by using biological materials, in particular, the invention relates to the technical field of rapidly constructing algal crusts by using mixed algae species. Background technique [0002] In recent years, my country is one of the countries with the most severe desertification in the world. The total area of ​​desertified land has reached 3.73 million square kilometers, and the annual expansion rate is 2460 square kilometers. Yuan. In view of this serious situation, it is urgent to find an effective way to prevent the desertification of land resources. [0003] Traditional windbreak and sand fixation measures such as afforestation, grass grid sand barriers and clay sand barriers are effective on the surface, but they cannot fundamentally solve the problem of desertification and desertification land governance. The main reason is that higher plants grow slowl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 张丙昌王敬竹张元明
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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