50 results about "Oscillatoria" patented technology

The invention discloses a separated purification method and the application thereof of a polycyclic aromatic hydrocarbon anaerobic degrading pure strain, which belongs to the polluted soil and aqueous biological degradation handling field. A base bottom layer flat plate is manufactured in the a anaerobic box filled with nitrogen gas by taking polycyclic aromatic hydrocarbon as a sole carbon source and an energy source and taking sodium nitrate or potassium nitrate as an electronic accepted anaerobic agar solid culture, the top layer flat plate of the anaerobic agar solid culture including polycyclic aromatic hydrocarbon anaerobic degrading mixed bacteria is manufactured on the bottom layer flat plate, the manufactured culture medium flat plate is put in a drying container filled with the nitrogen gas to culture one to two months after being sealed. Polycyclic aromatic hydrocarbon, electronic acceptance, basic culture medium, trace metal liquid, vitamin c solution and colony in the culture medium flat plate connected into the anaerobic bottle of resazurin are added in the anaerobic box filled with the nitrogen gas, and are cultured for 20 to 25 days in an oscillator under the temperature of 20 plus or minus 2 DEG C and the rotating speed of 100 r / min. Then, the cyclical switchover is performed, after a cycle time is larger than four times, the pure strain of the anaerobic degrading polycyclic aromatic hydrocarbon can be obtained. The method can obtain good microbe pure strain to the polycyclic aromatic hydrocarbon anaerobic degrading performance by being separated.

The invention discloses a domestication screening method and appliance of benzene series anaerobic degraded flora in polluted soil and water biological degraded disposing technical domain, which comprises the following steps: adding electron acceptor, sodium sulfide and BTEX mixture into polluted soil; sealing and stewing at three months; adding basic culture medium, trace metal liquid and vitamin C solution into anaerobic box filled with nitrogen; domesticating soil; switching domestication soil into anaerobic bottle with mass ratio of water and soil in the anaerobic box; adding into BTEX, electron acceptor and sodium sulfide with mass ratio; culturing 7-9 days in rotating oscillator with the temperature at 20+-2 deg.c; circling at least 9 times; getting the degraded flora. This invention can screen good anaerobic degraded property microbe flora, which can make degraded speed range of BTEX at 1.6-3.5mg.L-1.d-1.

The invention discloses a separation and purification method and application of aerobic degradation pure culture of polycyclic aromatic hydrocarbon, which belongs to the technical field of biodegradation treatment on polluted soil and water. A bottom flat for an agar solid culture medium taking the polycyclic aromatic hydrocarbon as unique carbon source and energy is prepared in a bechtop, and isprovided with a top flat for the agar solid culture medium containing an aerobic degradation microbial community of the polycyclic aromatic hydrocarbon, and the culture medium flats prepared are cultured in an incubator for 10 to 15 days; in the bechtop, a conical flask added with the polycyclic aromatic hydrocarbon, a basic medium, trace liquid metal, vitamin c solution and an adsorbent is inoculated with the microbial community in the medium flat, and the microbial community is cultured in an oscillator at a temperature of between 25 and 30DEG C and a speed of 100r/min for 5 to 7 days, and then is subjected to circular transfer inoculation; and the aerobic degradation pure culture of the polycyclic aromatic hydrocarbon can be obtained after the circulation times is more than 8. The method can separate and purify to obtain the microbial pure culture with good aerobic degradation performance on the polycyclic aromatic hydrocarbon.

The invention discloses a Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria. The strain is Bacillus cereus, is preserved in China Center for Type Culture Collection in Wuhan, China on April 9, 2013 as Bacillus cereus CZBC1, and has a preservation number of CCTCC NO:M2013130. The Bacillus cereus strain CZBC1 has obvious dissolving and eliminating effects on a plurality of dominant oscillatoria in culture water. The invention also discloses an application of the Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria in the dissolving of the pond oscillatoria.

The invention discloses a monad strain CZRST19 capable of dissolving pond oscillatoria. The monad strain CZRST19 is preserved in China Center for Type Culture Collection in Wuhan, China on April 9, 2013 as Stenotrophomonas maltophilia strain, and has a preservation number of CCTCC NO:M2013129. The Stenotrophomonas maltophilia strain CZRST19 has obvious dissolving and eliminating effects on a plurality of dominant oscillatoria in culture water. The invention also discloses an application of the Stenotrophomonas maltophilia strain CZRST19 capable of dissolving pond oscillatoria in the dissolving of the pond oscillatoria.

The invention discloses a preparation method of black fungus apple vinegar, which comprises the following steps: 1. inoculating a black fungus strain in a basal culture medium, and culturing at 28 DEG C for 7 days; 2. inoculating the activated strain into a sterilized liquid culture medium, standing at room temperature for 24 hours, putting into an oscillator, and culturing at 30 DEG C at the rate of 150 rpm for 8 days; 3. carrying out wall-breaking homogenization on the strain subjected to amplification culture twice, blending with apple juice subjected to heating sterilization, inoculating yeast to perform alcohol fermentation, and inoculating vinegar bacteria to perform acetic fermentation; and 4. after the fermentation finishes, filtering to obtain the black fungus apple vinegar. By utilizing the submerged fermentation technique, the method has the advantages of high productive capacity, small occupied area, short period and high controllability. The obtained product ingeniously combines the health care functions of the black fungus and fruit vinegar, has abundant nutrients and good taste, has the functions of nourishing and strengthening the body, enhancing the immunity and the like, and has higher nutritive value.

The invention discloses a preparation method of an ammonia nitrogen adsorbent by utilizing southern magnolia and the application for removing ammonia nitrogen in water body; southern magnolia leaves are used as raw material, and then 30-100 meshes of the adsorbent is obtained through collection, washing, airing, drying, crushing and sieving of the raw material; the adsorbent is mixed with ammonia nitrogen solution according to a certain proportion, the mixture is oscillated for 20 hours on an oscillator, so as to effectively remove the ammonia nitrogen in the water body. In the invention, the waste is reutilized, the raw material cost is low, the preparation and adsorption process are simple, and the method is suitable for sewage treatment of ammonia nitrogen with middle-low concentration.

It was observed that a highly purified preparation of polar glycolipids extracted from cells of the cyanobacterium Oscillatoria Planktothrix is characterized by the presence of at least one species of high molecular weight glycolipid comprising one or more units of rhamnose. In such mixture, having a level of nucleic acid contamination lower than (or equal to) 3%, an inhibitory activity toward ATP synthase (ATP-SX) was identified which is capable of decreasing the level of extracellular ATP and thereby the extent of the inflammatory response. The glycolipid mixture is especially useful in inflammation induced by ischemic stimuli, cancer or autoimmune diseases (such as multiple sclerosis, inflammatory bowel disease (Crohn's disease, ulcerative colitis), psoriasis, rheumatoid arthritis, diabetes, autoimmune thyroiditis, systemic lupus erythematosus), in addition to systemic inflammatory states such as systemic inflammatory syndrome (SIRS), sepsis, vasculitis, or in localized inflammatory states, such as neurodegenerative diseases or asthma.

The invention discloses a method for preparing ammonia nitrogen sorbent by utilizing abandoned strawberry leaves and the application of the sorbent. The abandoned strawberry leaves are used as raw materials, and the raw materials are washed, aired, dried and crashed and then are sieved through a standard sieve to obtain the sorbent with the mesh number of 30 to 200. The sorbent is added to a water solution containing ammonia nitrogen and is surged for 20 hours on an oscillator, and the ammonia nitrogen in the water can be effectively removed. The raw materials of the sorbent recycles agricultural wastes, the cost is low, the preparation process is simple, and the raw materials have highly specific adsorption performance to the ammonia nitrogen in the water.

The invention discloses an industrial liquid-solid combined fermentation method for bacillus cereus preparation for dissolving oscillatoria in an aquaculture pond. According to the invention, industrial liquid fermentation and solid fermentation systems of a microbial inoculum are taken as the platform, a production technology process including seed solution culturing, liquid-solid combined fermentation of thalli, drying and smashing of the fermented product, and packaging to obtain the finished product is established, a system solution for the industrial liquid-solid combined fermentation production of the bacillus cereus preparation for dissolving oscillatoria is provided, and the relevant microbial inoculum product is formed. According to the method, the characteristics of high efficiency and high purity and the liquid fermentation method are reserved, the low-cost effect of the solid fermentation method is also considered, and thus the production cost of the microbial inoculum is reduced; meanwhile, the influences on the algae-lytic characteristic and other ecological characteristics of the bacillus cereus preparation caused by the industrial scale are avoided, and the microbial inoculum product can be applied to the aquaculture pond for preventing and controlling cyanobacterial bloom.

The present invention relates to the preparation and use of a glycolipid fraction from Oscillatoria Planktothrix sp., for the treatment and/or prevention of bacterial gum diseases primarily caused by: Actinobacillum actinomycetemconcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and even more preferably by Porphyromonas gingivalis. Said gum diseases, in particular gingivitis and periodontitis (pyorrhea), are primarily caused by a pro-inflammatory response to components of P. gingivalis, leading to destruction of periodontal tissue, and are often accompanied by osteoclastogenesis (increased number of osteoclasts responsible for destruction of bone tissue), and by chronic infection.

The invention discloses a Fermentation method of alternaria tenuissima. The fermentation method comprises the following steps: taking out an alternaria tenuissima culture HZ-1 stored at 4 DEG C; picking up part of bacterial colony and inoculating the same to a fresh PDA culture medium to be cultured, then picking up part of the bacterial colony from the culture medium and inoculating the same to afresh PDA culture medium, and repeating the step 2-3 times to obtain an activated HZ-1 culture which is vigorous in growing power; taking the HZ-1 culture cultured for 7d in a petri dish, perforating8mm bacterial cakes by a perforator, and inoculating the bacterial cakes 10/L into the PDA liquid culture medium; and culturing the cakes in an oscillator, and filtering the mixture to obtain a HZ-1fermenting liquid. According to the fermentation method, the culture HZ-1 is reproduced massively, thereby laying a theoretical foundation for industrial production and further popularization and application of a biological fungicide.

The invention discloses a photosynthetic type microbial preparation for aquaculture. The photosynthetic type microbial preparation for aquaculture is prepared from the following components in parts by weight: 8-10 parts of chroococcoid, 6-8 parts of oscillatoria, 6-8 parts of nostoc, 5-7 parts of anabaena, 4-5 parts of spiral seaweeds, 1-2 parts of rhodospirillum, 1-2 parts of rhodop seudanonas palustris and 1-2 parts of red micro bacteria. In this way, the photosynthetic type microbial preparation for aquaculture has the advantages of being complete in disinfection, cutting off viral infection, purifying water quality, achieving harmless standards, being natural and free of toxicity, increasing an aquaculture survival rate, and the like, and has a wide market prospect on popularization of the photosynthetic type microbial preparation for aquaculture.

The invention belongs to the field of animal husbandry and veterinary and in particular relates to a method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of the method. The method comprises the following steps: feeding breeder flocks in single cages and encoding; collecting primary hatching eggs of the breeder flocks; enabling the primary hatching eggs to correspond to numbers of the hatching eggs one by one; disinfecting the surfaces of the hatching eggs and opening egg shells from hatching egg air chamber ends;sucking egg white and yolks and putting into PE (Polyethylene) tubes respectively and marking; putting egg white and yolk samples into a refrigerator and freezing and thawing for a plurality of times; taking the frozen and thawed egg white; detecting an ALV virus antigen through an avian leukosis ELISA (Enzyme Linked Immunosorbent Assay) detection method; taking the frozen and thawed yolks and adding normal saline and chloroform; after uniformly mixing on an oscillator, centrifuging and layering a solution, wherein a lower layer is an organic solvent, a middle layer is denatured lipid proteinand an upper layer is an extracted antibody; and sucking the antibody of the upper layer and detecting a salmonellosis antibody through a plate agglutination method; and eliminating chicken flock individuals with positive detection results of the ALV virus antigen and the salmonellosis antibody.

The invention provides a method for extracting streptomyces alga dissolution active substances, and belongs to the field of eutrophic water body bioremediation. An isometric absolute ethyl alcohol isused for repeatedly extracting streptomyces fermentation liquor filtrate, after centrifugalization is completed, a rotary evaporator is used for concentrating an organic phase to be dry, crude substances are obtained, then methyl alcohol is used for dissolving the crude substances, and silica gel column chromatography separation is carried out; the components with efficient alga dissolution activity are subjected to high-performance liquid chromatographic separation, and the alga dissolution active substances are obtained. Adopted streptomyces comes from an eutrophic water body, the alga dissolution active substances secreted by the streptomyces are secondary metabolites, high safety is achieved, and no secondary pollution is caused to the water body. The alga dissolution active substancescan be used for treatment of the eutrophic water body caused by typical harmful algae such as microcystis, anabaena, aphanizomenon and oscillatoria. The extraction method is simple in process, high in operability, low in production cost, good in alga dissolution effect and suitable for large-scale production.

The invention discloses a method for domesticating and screening a degrading flora for petroleum hydrocarbon from sweater, which belongs to the technical field of the biological degradation treatment of the seawater polluted by spilled oil, which comprises the following steps: adding the seawater into an open glass bottle into which crude oil, a basic culture medium, a trace metal liquid and a vitamin c solution are added, carrying out the enrichment and the domestication of the degrading flora for the petroleum hydrocarbon for 10-12 months, and then, introducing an enriched and domesticated seawater sample into a conical flask into which the crude oil, the basic culture medium, the trace metal liquid and the vitamin c solution are added; culturing the seawater sample for 6-8 days in an oscillator at a temperature of 10-15 DEG C and a rotational speed of 100 r/min, and then, carrying out circular transfer, so as to obtain the degrading flora for efficiently removing the petroleum hydrocarbon in the seawater polluted by the spilled oil after circulation times are more than 6 times.

The invention provides application of the fumaric acid in inhibiting the growth of the blue-green algae. In a blue-green algae water area with a certain concentration, the growth of the blue-green algae can be inhibited for a long time by applying 40mg/L fumaric acid. Compared with common small molecule acid inhibitors (acetic acid and hydrochloric acid), the inhibitor has the advantages of good inhibition effect, no secondary pollution and high ecological safety. In addition, fumaric acid not only can inhibit the water bloom dominant species microcystis aeruginosa, but also has an inhibiting effect on oscillatoria, and has general applicability to blue-green algae. Fumaric acid can effectively control blue-green algae for a long time, and is low in cost, environment-friendly and suitable for being popularized to the society.

The invention discloses a method for extracting flavonoid substances in penthorum chinense pursh. The method comprises the steps that (1), the penthorum chinense pursh is smashed into fine powder withthe particle size of being 40 mesh or above; (2), the fine powder is dried for 4-6 hours at the temperature of 60-80 DEG C; (3), a methyl alcohol solution is added, a nanometer metal material is added, micro waves are applied, after uniform mixing, water-bath extraction is conducted for 1.5 hours at the extraction temperature of 60-80 DEG C by using a refluxing device, and then the mixture is taken out and subjected to vacuum filtration to obtain filter liquor; (4), pretreated polyamide resin and macroporous resin are weighed, the filter liquor obtained in the step 3 is added to obtain a mixture, the mixture is placed into an oscillator for oscillation for 12 hours at the temperature of 30 DEG C, elution is conducted by using a solvent, an eluant is collected, concentrated and evaporatedto dryness, and then the extracts are obtained.

The invention relates to a method for increasing the egg laying amount of arma chinensis. The method comprises the following steps that 1) newly laid arma chinensis eggs are placed in a centrifuge tube; 2) after an ethanol solution is added into the centrifuge tube in step 1), and the centrifuge tube is placed in an oscillator for three-time oscillation treatment; 3) after a formaldehyde solutionis added into the centrifuge tube in step 2), the centrifuge tube is placed in the oscillator for oscillation treatment; 4) after the ethanol solution is added into the centrifuge tube in step 3), thecentrifuge tube is placed in the oscillator for oscillation treatment; 5) after the obtained arma chinensis eggs in step 4) are rinsed thoroughly with a sterilized deionized water, the surface moisture is sucked dry with sterilization filter paper. The method for increasing the egg laying amount of arma chinensis has the advantages that after the arma chinensis eggs treated through the experimental steps are hatched into adult arma chinensis, under the conventional breeding conditions of a laboratory, the egg laying amount is effectively increased, thereby being conductive to the expanding propagation of arma chinensis population, and saving the economics, time and labor cost of arma chinensis raising.