Application of fumaric acid in inhibiting growth of blue-green algae

A technology of fumaric acid and cyanobacteria, applied in the direction of application, plant growth regulator, and chemicals for biological control, etc., to achieve the effect of wide sources, low price, and environmental friendliness

Inactive Publication Date: 2021-06-25
NANJING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to the above applications, fumaric acid is also an effective fungicide, but so far there has been no report on its application as an algicide

Method used

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  • Application of fumaric acid in inhibiting growth of blue-green algae
  • Application of fumaric acid in inhibiting growth of blue-green algae
  • Application of fumaric acid in inhibiting growth of blue-green algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Inhibitory effect of fumaric acid on stable stage Microcystis aeruginosa FACHB-315.

[0061] Add 75mL of BG11 medium to a sterilized 250mL Erlenmeyer flask in a sterile operating bench, add an equal volume of FA stock solution (experimental group) or BG11 medium (control group) with different concentrations, and incubate until stable Stage Microcystis aeruginosa FACHB-315, so that the initial optical density of the final algae liquid OD 680 is 0.08 and the dosage of FA is 0mg / L (control group), 1mg / L, 15mg / L, 30mg / L and 40mg / L respectively, wherein each control group and experimental group set up 3 parallel samples, and the algae The solution was cultured in a light incubator with a light intensity of 2800Lx, a temperature of 25±1°C, and a light-to-dark ratio of 12:12. It was shaken regularly 3 times a day and placed randomly. Samples were taken at 0d at the beginning of the culture and at 2d, 4d, 6d and 8d during the culture and tested for their optical density OD 68...

Embodiment 2

[0066] Inhibitory effect of fumaric acid on log-phase Microcystis aeruginosa FACHB-315.

[0067] The operation steps are the same as those in Example 1, except that the algae cells inserted are Microcystis aeruginosa FACHB-315 cultured to the logarithmic phase.

[0068] Figure 4 It is the change of optical density and corresponding inhibition rate of Microcystis aeruginosa FACHB-315 within 8 days (including 8 days). The growth trend of algae species in the stationary phase was basically consistent, but the inhibition rate of the 15mg / L and 30mg / L FA experimental groups on the algae species in the logarithmic phase was significantly higher than that of the inoculated algae in the stable phase. Similarly, the 1mg / L FA experimental group still had no significant difference with the control group, and the 40mg / L FA experimental group was significantly inhibited and continued to die. This shows that the same concentration of FA has a better inhibitory effect on inoculated algae ...

Embodiment 3

[0070] Inhibitory effects of different small molecule acid inhibitors on Microcystis aeruginosa FACHB-315 in the stationary phase.

[0071] Add 75mL of BG11 medium to a sterilized 250mL Erlenmeyer flask in a sterile operating bench, add FA, acetic acid, hydrochloric acid stock solution (experimental group) or BG11 medium (control group), and inoculate and culture until stable Stage Microcystis aeruginosa FACHB-315, so that the initial optical density of the final algae liquid OD 680 It is 0.08 and the dosage of inhibitor is 40mg / L. It is worth noting that the pH of the system after adding hydrochloric acid is equal to the pH after adding FA. In each control group and experimental group, 3 parallel samples are set, and the algae The solution was cultured in a light incubator with a light intensity of 2800Lx, a temperature of 25±1°C, and a light-to-dark ratio of 12:12. It was shaken regularly 3 times a day and placed randomly. Samples were taken at 0d at the beginning of the cu...

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PUM

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Abstract

The invention provides application of the fumaric acid in inhibiting the growth of the blue-green algae. In a blue-green algae water area with a certain concentration, the growth of the blue-green algae can be inhibited for a long time by applying 40mg/L fumaric acid. Compared with common small molecule acid inhibitors (acetic acid and hydrochloric acid), the inhibitor has the advantages of good inhibition effect, no secondary pollution and high ecological safety. In addition, fumaric acid not only can inhibit the water bloom dominant species microcystis aeruginosa, but also has an inhibiting effect on oscillatoria, and has general applicability to blue-green algae. Fumaric acid can effectively control blue-green algae for a long time, and is low in cost, environment-friendly and suitable for being popularized to the society.

Description

technical field [0001] The invention belongs to the field of algae inhibitors, in particular to the application of fumaric acid in inhibiting the growth of cyanobacteria in eutrophic water bodies. Background technique [0002] Nowadays, the frequent occurrence of cyanobacteria "bloom" in various water sources has become a global problem that has attracted widespread attention and urgently needs to be solved. The outbreak of "Water Bloom" not only increases the turbidity of the water body and affects the visual appreciation, but also produces toxins and odor substances, which seriously affects the aquatic ecosystem and threatens human health. Therefore, it is of great significance to study how to effectively control the growth of algae, improve the water quality of eutrophic water bodies, and restore a healthy aquatic ecosystem. [0003] Microcystis aeruginosa has pseudovacuoles, which can reproduce in large numbers by adjusting buoyancy to obtain suitable growth conditions....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/06A01P13/00
CPCA01N37/06
Inventor 瞿晓磊魏培云付翯云
Owner NANJING UNIV
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