Separation and purification method and application of aerobic degradation pure culture of polycyclic aromatic hydrocarbon

A technology for separation and purification of polycyclic aromatic hydrocarbons, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc. High toxicity of microorganisms and other issues

Inactive Publication Date: 2010-02-24
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polycyclic aromatic hydrocarbons, especially fused-ring aromatic hydrocarbons higher than four rings, have very low water solubility and high toxicity to microorganisms, which makes the traditional separation and purification methods of microbial strains unsuitable for pure strains of PAH degradation. Isolation and Purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Utilize the bacterial classification that the inventive method separates and purifies has carried out the aerobic degradation test of naphthalene under aerobic condition, the result shows that the pure bacterial classification that isolates can carry out effective aerobic degradation to naphthalene, when the initial concentration of naphthalene is 26.1mg / At L, it can be degraded to below 0.02mg / L after 2 days.

Embodiment 2

[0021] Utilize the strains of separation and purification of the inventive method to carry out the aerobic degradation test of phenanthrene under aerobic condition, the result shows that the pure strain that isolates can carry out effective aerobic degradation to phenanthrene, when the initial concentration of phenanthrene is 1.05mg / At L, it can be degraded to below 0.01mg / L after 2 days.

Embodiment 3

[0023] The bacteria strains separated and purified by the method of the present invention were tested for aerobic degradation of fluorene under aerobic conditions. The results showed that the isolated pure strains could effectively degrade fluorene. When the initial concentration of fluorene was 0.11mg / At L, it can be degraded to below 0.01mg / L after 1 day.

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PUM

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Abstract

The invention discloses a separation and purification method and application of aerobic degradation pure culture of polycyclic aromatic hydrocarbon, which belongs to the technical field of biodegradation treatment on polluted soil and water. A bottom flat for an agar solid culture medium taking the polycyclic aromatic hydrocarbon as unique carbon source and energy is prepared in a bechtop, and isprovided with a top flat for the agar solid culture medium containing an aerobic degradation microbial community of the polycyclic aromatic hydrocarbon, and the culture medium flats prepared are cultured in an incubator for 10 to 15 days; in the bechtop, a conical flask added with the polycyclic aromatic hydrocarbon, a basic medium, trace liquid metal, vitamin c solution and an adsorbent is inoculated with the microbial community in the medium flat, and the microbial community is cultured in an oscillator at a temperature of between 25 and 30DEG C and a speed of 100r/min for 5 to 7 days, and then is subjected to circular transfer inoculation; and the aerobic degradation pure culture of the polycyclic aromatic hydrocarbon can be obtained after the circulation times is more than 8. The method can separate and purify to obtain the microbial pure culture with good aerobic degradation performance on the polycyclic aromatic hydrocarbon.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, and in particular relates to a separation and purification method and application of pure strains for aerobic degradation of polycyclic aromatic hydrocarbons. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to hydrocarbon compounds composed of two or more benzene rings linked together. They are a class of organic compounds that are carcinogenic, teratogenic, and mutagenic. Sex has thus attracted widespread attention. Because of the conjugated large π-bond electron system in PAHs molecules, they have high stability and are usually difficult to be degraded by microorganisms. In order to increase the biodegradation rate of PAHs, it is very important to domesticate and screen out efficient degrading bacteria. Aerobic biodegradation of PAHs is many times faster than anaerobic biodegradation, so aerobic biological treatment techn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C02F3/34C02F3/02B09C1/10C12R1/01C12R1/645C02F101/32
CPCY02W10/10
Inventor 豆俊峰丁爱中
Owner BEIJING NORMAL UNIVERSITY
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