Enrichment, domestication and screening method and application of aerobic degradation microbial community of polycyclic aromatic hydrocarbon

A technology for polycyclic aromatic hydrocarbons and screening methods, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of low water solubility, unsuitable degradation of polycyclic aromatic hydrocarbons microbiota domestication and screening, High toxicity of microorganisms and other issues

Inactive Publication Date: 2010-02-24
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Polycyclic aromatic hydrocarbons, especially fused-ring aromatic hydrocarbons higher than four rings, have very low water solubility and high toxicity to microorganisms, which makes conventional domestication and screening microbial flora techniques unsuitable for degrading polycyclic aromatic hydrocarbons. domestication and screening

Method used

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Embodiment

[0018] The aerobic degradation test of polycyclic aromatic hydrocarbons has been carried out using the flora domesticated by the inventive method, and the results show that the flora can carry out effective aerobic degradation to naphthalene, phenanthrene, fluorene and pyrene; when the initial concentration of naphthalene is 27.4mg / L , after 5 days, it can be degraded to below 0.01mg / L. When the initial concentration of phenanthrene is 1.01mg / L, it can be degraded to below 0.05mg / L after 2 days. Degrade to below 0.01mg / L, when the initial concentration of pyrene is 0.12mg / L, can degrade to below 0.01mg / L after 2 days; The aerobic degradation rates of naphthalene, phenanthrene, fluorene and pyrene are respectively (thalline concentration is 5×10 7 Individual / mL): 5.46mg·L -1 d -1 ;0.48mg·L -1 d -1 ;0.05mg·L -1 d -1 and 0.06mg·L -1 d -1 .

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Abstract

The invention discloses an enrichment, domestication and screening method and application of an aerobic degradation microbial community of polycyclic aromatic hydrocarbon. An open glass bottle added with the polycyclic aromatic hydrocarbon and a basic medium is added with soil polluted by petroleum to enrich and domesticate aerobic degradation microbes of the polycyclic aromatic hydrocarbon; after30 days, a conical flask added with polycyclic aromatic hydrocarbon, a basic medium, trace liquid metal, vitamin c solution and an adsorbent is inoculated with the soil after the enrichment and domestication, and the soil is cultured in an oscillator at a temperature of between 25 and 30DEG C and a speed of 100r / min for 5 to 7 days, and then is subjected to circular transfer inoculation; and theaerobic degradation microbial community capable of highly efficiently removing the polycyclic aromatic hydrocarbon can be obtained after the circulation times is more than 8. The method can enrich, domesticate and screen the microbial community with good aerobic degradation performance on the polycyclic aromatic hydrocarbon, and aerobic degradation rates of the microbial community on naphthaline,phenanthrene, fluorine and pyrene are respectively 5.46mg.L<-1>.d<-1>, 0.48mg.L<-1>.d<-1>, 0.05mg.L<-1>.d<-1> and 0.06mg.L<-1>.d<-1>.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, and in particular relates to a method for enrichment, domestication and screening of polycyclic aromatic hydrocarbon aerobic degradation bacteria and its application. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to hydrocarbon compounds composed of two or more benzene rings linked together. They are a class of organic compounds that are carcinogenic, teratogenic, and mutagenic. Sex has thus attracted widespread attention. Because of the conjugated large π-bond electron system in PAHs molecules, they have high stability and are usually difficult to be degraded by microorganisms. In order to increase the biodegradation rate of PAHs, it is very important to domesticate and screen out efficient degrading bacteria. Aerobic biodegradation of PAHs is many times faster than anaerobic biodegradation, so aerobic biological treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12Q1/04C02F3/34C02F3/02B09C1/10C12R1/01C12R1/645C02F101/32
Inventor 豆俊峰丁爱中
Owner BEIJING NORMAL UNIVERSITY
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