A Stenotrophomonas maltophilia strain czrst19 for dissolving Oscillatoria in ponds and its application

A technology of Stenotrophomonas and Stenotrophomonas genus, applied in the direction of bacteria, microbial-based methods, water/sludge/sewage treatment, etc., can solve the problem of ignoring interconnections, industrial application limitations, and maltophilic oligosaccharides. Whether the strains of Trotrophomonas can dissolve Oscillator algae in ponds and other issues, to achieve the effect of good algalysis specificity, good dissolution effect, and remarkable ecological benefits

Active Publication Date: 2014-10-08
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Some scholars have explored the microalgae phase regulation technology in shrimp culture ponds (Corre, 2005; Cremen, 2007; Yusoff, 2002), and analyzed the relevant microalgae physiological characteristics and competition characteristics among algae (Kuwata, 2000; Klausmeier ,2004;Smith,1983;Piazzi,2002;Litchman,2003), but most of them ignore the interrelationship among "environmental nutrition-microbes-microalgae", which is quite different from the actual pond environment. The application level is also subject to certain restrictions
And it has not been studied whether the strain of Stenotrophomonas maltophilia can dissolve the pond Oscillatoria

Method used

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  • A Stenotrophomonas maltophilia strain czrst19 for dissolving Oscillatoria in ponds and its application
  • A Stenotrophomonas maltophilia strain czrst19 for dissolving Oscillatoria in ponds and its application
  • A Stenotrophomonas maltophilia strain czrst19 for dissolving Oscillatoria in ponds and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The screening method of the Stenotrophomonas maltophilia bacterial strain CZRST19 of embodiment 1 dissolving Oscillatoria pond:

[0019] 1. Material preparation

[0020] 1. Bacterial source

[0021] Obtained from Maoming Dianbai Farm;

[0022] 2. Medium

[0023] (1) Microalgae BG11 liquid medium;

[0024] (2) Add NH to sterilized pond water 4 Cl1.0~1.5g / L and Na 2 HPO 4 30~40mg / L.

[0025] 2. Isolation and screening of strains

[0026] In a farm in Dianbai, Maoming, the dominant cyanobacteria in the pond - Microcystis blooms, Microcystis aeruginosa, planktonic Oscillatoria, and Oscillatoria microcystis were purely cultivated. Cultured in two kinds of culture medium, one is BG11 liquid medium, the other is sterilized pond water with NH 4 Cl1.0~1.5g / L and Na 2 HPO 4 30~40mg / L. The temperature is 25-28°C, the light intensity is 2500-4000lx, and the light-dark cycle is 12h:12h, and cultured for 5-7 days. The algae liquid was diluted in gradient, spread on the...

Embodiment 2

[0029] Example 2 Identification of Stenotrophomonas maltophilia Strain CZRST19 Dissolving Pond Oscillatoria

[0030] The present invention carries out physiological and biochemical identification and 16S rDNA molecule identification on the interalgal algae-dissolving strain CZRST19 which has the function of dissolving Oscillator algae, and determines the species of the strain from the molecular level, combined with the analysis of bacterial morphological characteristics and physiological and biochemical characteristics. 16S rDNA sequence analysis mainly follows the following steps:

[0031] Extraction of bacterial genomic DNA:

[0032] 1. Use an autoclaved toothpick to pick a single colony and inoculate it in the expansion medium for overnight cultivation;

[0033] 2. Take 1.5 mL of bacterial culture solution, centrifuge at 10,000 rpm (11,500×g) for 1 minute, and suck up the supernatant as much as possible;

[0034] 3. Add 200 μL of buffer GA to the cell pellet, shake until th...

Embodiment 3

[0081] Example 3 Laboratory Small-scale Application of Stenotrophomonas maltophilia Strain CZRST19 Dissolving Pond Oscillatoria

[0082] The CZRST19 strain was cultured at 30°C and 200r / min for 20 hours, and then added to the pure culture algae liquid of fine microalgae—Chlorella pyrenoidosa, Scenedesmus tetracinus, Cyclotella stripes, and Nitzschia crescentus. concentration is 10 7 cfu / mL, the cell density of each algae fluid was 1.6×10 6 cell / mL, 6.0×10 5 cell / mL, 2.3×10 5 cell / mL, 5.3×10 5 cell / mL. The mixture of bacteria and algae was cultured for 8 days at 28°C, 2000lx, and a light-dark cycle of 12h:12h. Results The cell numbers of the four algae were 8.05×10 6 cell / mL, 2.95×10 6 cell / mL, 3.75×10 6 cell / mL, 3.68×10 6 cell / mL. It can be seen that CZRST19 only has a lytic effect on specific Oscillatoria, but has no lytic and killing effect on the above four microalgae, but compared with the control group without bacteria, the growth of the four algae is inhibited ...

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Abstract

The invention discloses a monad strain CZRST19 capable of dissolving pond oscillatoria. The monad strain CZRST19 is preserved in China Center for Type Culture Collection in Wuhan, China on April 9, 2013 as Stenotrophomonas maltophilia strain, and has a preservation number of CCTCC NO:M2013129. The Stenotrophomonas maltophilia strain CZRST19 has obvious dissolving and eliminating effects on a plurality of dominant oscillatoria in culture water. The invention also discloses an application of the Stenotrophomonas maltophilia strain CZRST19 capable of dissolving pond oscillatoria in the dissolving of the pond oscillatoria.

Description

technical field [0001] The invention belongs to the technical field of using microorganisms to control microalgae, and in particular relates to Stenotrophomonas maltophilia strain CZRST19 for dissolving Oscillatoria in ponds and its application. Background technique [0002] Microalgae are indispensable for maintaining the normal function of the water ecosystem, promoting the absorption and transformation of environmental nitrogen and phosphorus nutrients, and stabilizing the water environment (Li Zhuojia, 2005, Wang Chaohui, etc., 1999). In terms of production performance, ponds dominated by cyanobacteria tend to deteriorate in water quality, and prawns grow slowly and are prone to diseases (Peng Congcong, 2010, Liu Xiaozhu et al., 2011); ponds dominated by green algae have stable water quality and fewer shrimp diseases , the prawns grow well, and the water ecosystem is relatively stable (Huang Xianghu et al., 2002, Zha Guangcai et al., 2004, Shen Yuchun et al., 2004); in p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 曹煜成王丽花李卓佳文国樑胡晓娟
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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