Method of domesticating and screening benzene series anaerobic degradation flora

A screening method, a technology for degrading bacteria, applied in anaerobic digestion treatment, bacteria, biological sludge treatment, etc., can solve the problem of refractory biodegradation, domestication and enrichment technology is not applicable to the anaerobic degradation microbial group domestication screening of benzene series And other issues

Inactive Publication Date: 2007-08-15
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Li Xiaoming, Chen Jian, Lun Shiyi, etc. in "Environmental Science and Technology, 2001, 3: 21-24", "Rapid domestication and enrichment technology of anaerobic microorganisms degrading terephthalic acid", which is most relevant to this application However, because benzene series, especially benze...

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Embodiment 1

[0020] Utilize the mixed flora domesticated by the inventive method to carry out the anaerobic degradation test of benzene series compound (BTEX) under the nitrate reduction condition, the result shows that mixed flora can treat benzene, toluene, ethylbenzene, o-xylene, m-xylene and p-xylene for effective anaerobic degradation. When the initial concentration is less than 100mg / L, it can be degraded to below 0.2mg / L after 60 days. The maximum anaerobic degradation rates of benzene, toluene, ethylbenzene, o-xylene, m-xylene and p-xylene are respectively (the cell concentration is 10 8 Individual / mL): 2.44mg·L -1 d -1 ;3.54mg·L -1 d -1 ;3.43mg·L -1 d -1 ; 2.73mg·L -1 d -1 ; 2.84mg·L -1 d -1 and 2.54mg·L -1 d -1 .

Embodiment 2

[0022]Utilize the mixed flora domesticated by the inventive method to carry out the anaerobic degradation test of benzene series compound (BTEX) under sulfate reduction condition, the result shows that mixed flora can be to benzene, toluene, ethylbenzene, o-xylene, m-xylene and p-xylene for effective anaerobic degradation. When the initial concentration is less than 100mg / L, it can be degraded to below 0.2mg / L after 70 days. The anaerobic degradation rates of benzene, toluene, ethylbenzene, o-xylene, m-xylene and p-xylene are respectively (the cell concentration is 10 8 Individual / mL): 2.23mg·L -1 d -1 , 3.31mg·L -1 d -1 , 3.08mg·L -1 d -1 , 2.52mg·L -1 d -1 , 2.71mg·L -1 d -1 and 2.11mg·L -1 d -1 .

Embodiment 3

[0024] Utilize the mixed flora domesticated by the method of the present invention in Fe 3+ The anaerobic degradation test of benzene series compounds (BTEX) was carried out under reducing conditions, and the results showed that the mixed bacterial flora could effectively anaerobically degrade benzene, toluene, ethylbenzene, o-xylene, m-xylene and p-xylene. When the initial concentration is less than 100mg / L, it can be degraded to below 0.2mg / L after 80 days. The anaerobic degradation rates of benzene, toluene, ethylbenzene, o-xylene, m-xylene and p-xylene are respectively (the cell concentration is 10 8 Individual / mL): 1.87mg·L -1 d -1 , 2.68mg·L -1 d -1 , 2.42mg·L -1 d -1 , 2.06mg·L -1 d -1 , 2.21mg·L -1 d -1 and 1.63mg·L -1 d -1 .

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Abstract

The invention discloses a domestication screening method and appliance of benzene series anaerobic degraded flora in polluted soil and water biological degraded disposing technical domain, which comprises the following steps: adding electron acceptor, sodium sulfide and BTEX mixture into polluted soil; sealing and stewing at three months; adding basic culture medium, trace metal liquid and vitamin C solution into anaerobic box filled with nitrogen; domesticating soil; switching domestication soil into anaerobic bottle with mass ratio of water and soil in the anaerobic box; adding into BTEX, electron acceptor and sodium sulfide with mass ratio; culturing 7-9 days in rotating oscillator with the temperature at 20+-2 deg.c; circling at least 9 times; getting the degraded flora. This invention can screen good anaerobic degraded property microbe flora, which can make degraded speed range of BTEX at 1.6-3.5mg.L-1.d-1.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, and in particular relates to a domestication screening method and application of benzene series anaerobic degradation bacterial group. Background technique [0002] Benzene series mainly include the aerobic biodegradation of benzene, toluene, ethylbenzene, o-xylene, m-xylene and p-xylene (benzene, toluene, ethylbenzene, m-xylene, o-xylene, p-xylene, BTEX for short) is the fastest. Reported in literature 1 Shinoda Y, Sakai Y, Uenishi H, et al.2004.Aerobic and Anaerobic Toluene Degradation by a NewlyIsolated DenitrifyingBacterium, Thauera sp. Strain DNT-1. Applied and environmental microbiology, 70(3):1385-1392" , benzene series can be completely mineralized to CO under aerobic conditions 2 , however, underground aquifers or polluted soils are usually in an anaerobic state, so that aerobic microorganisms in the polluted zone are replaced by anaerobic mi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/28C02F11/04C09C1/10
Inventor 刘翔豆俊峰邓栋
Owner TSINGHUA UNIV
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