Method for domesticating and screening degrading flora for petroleum hydrocarbon from seawater

A technology of petroleum hydrocarbon degrading bacteria and degrading bacteria, which is applied in the direction of using microorganisms, etc., can solve the problems of domestication and screening of petroleum hydrocarbon microorganisms that cannot be applied

Inactive Publication Date: 2012-01-25
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, conventional techniques for domestication and screening of microbial flora cannot be applied to the

Method used

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Embodiment

[0018] The petroleum hydrocarbon degrading flora obtained by using the method of the present invention domesticated and screened has carried out the degradation test of petroleum hydrocarbons in the oil spill polluted seawater, and the results show that the flora can effectively degrade petroleum hydrocarbons: when the initial concentration of petroleum hydrocarbons is 6.9g / At L, it can be degraded to 2.1g / L after 6 days, and the degradation rate to petroleum hydrocarbons can reach 69.56%.

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Abstract

The invention discloses a method for domesticating and screening a degrading flora for petroleum hydrocarbon from sweater, which belongs to the technical field of the biological degradation treatment of the seawater polluted by spilled oil, which comprises the following steps: adding the seawater into an open glass bottle into which crude oil, a basic culture medium, a trace metal liquid and a vitamin c solution are added, carrying out the enrichment and the domestication of the degrading flora for the petroleum hydrocarbon for 10-12 months, and then, introducing an enriched and domesticated seawater sample into a conical flask into which the crude oil, the basic culture medium, the trace metal liquid and the vitamin c solution are added; culturing the seawater sample for 6-8 days in an oscillator at a temperature of 10-15 DEG C and a rotational speed of 100 r/min, and then, carrying out circular transfer, so as to obtain the degrading flora for efficiently removing the petroleum hydrocarbon in the seawater polluted by the spilled oil after circulation times are more than 6 times.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of seawater polluted by oil spills, in particular to a method for acclimating and screening petroleum hydrocarbon degrading bacteria from seawater. Background technique [0002] In recent years, with the increasing demand for oil and its products in various countries around the world, oil spill accidents have occurred frequently in the process of offshore mining, loading and unloading, transportation and utilization of oil, which not only caused a large amount of crude oil loss, but also caused serious pollution to the environment. It is increasingly threatening the ecological environment of the ocean and land, and has long-term harm to the marine environment, natural resources and aquaculture resources. Therefore, marine oil spill pollution has become a major environmental problem that people must face. Among marine oil spill treatment technologies, bioremediation technology has ...

Claims

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Application Information

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IPC IPC(8): C12N1/26
Inventor 豆俊峰马梦娇程莉蓉丁爱中郑蕾李帅冉王鸿婷范福强
Owner BEIJING NORMAL UNIVERSITY
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